Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_0190

Experiment: M9 with sucrose and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_0189 and EX28DRAFT_0190 are separated by 10 nucleotidesEX28DRAFT_0190 and EX28DRAFT_0191 are separated by 556 nucleotides EX28DRAFT_0189: EX28DRAFT_0189 - succinyldiaminopimelate desuccinylase (EC 3.5.1.18), at 138,284 to 139,411 _0189 EX28DRAFT_0190: EX28DRAFT_0190 - transcriptional regulator, Spx/MgsR family, at 139,422 to 139,772 _0190 EX28DRAFT_0191: EX28DRAFT_0191 - The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family, at 140,329 to 143,442 _0191 Position (kb) 139 140Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 139.469 kb on + strand, within EX28DRAFT_0190at 139.470 kb on - strand, within EX28DRAFT_0190at 139.800 kb on + strandat 139.801 kb on - strandat 139.824 kb on - strandat 139.842 kb on + strandat 139.869 kb on - strandat 139.929 kb on - strandat 140.047 kb on + strandat 140.101 kb on + strandat 140.140 kb on + strandat 140.143 kb on + strandat 140.146 kb on + strandat 140.266 kb on - strandat 140.275 kb on + strandat 140.394 kb on - strandat 140.536 kb on + strandat 140.537 kb on - strandat 140.537 kb on - strandat 140.672 kb on + strand, within EX28DRAFT_0191at 140.672 kb on + strand, within EX28DRAFT_0191at 140.673 kb on - strand, within EX28DRAFT_0191

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM
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139,469 + EX28DRAFT_0190 0.13 -3.1
139,470 - EX28DRAFT_0190 0.14 +0.3
139,800 + +1.3
139,801 - +0.0
139,824 - +0.1
139,842 + +0.5
139,869 - -0.9
139,929 - +0.1
140,047 + +1.9
140,101 + +0.3
140,140 + -1.3
140,143 + -0.2
140,146 + +0.1
140,266 - -0.1
140,275 + +0.1
140,394 - -0.2
140,536 + -0.2
140,537 - +1.3
140,537 - -0.1
140,672 + EX28DRAFT_0191 0.11 -0.5
140,672 + EX28DRAFT_0191 0.11 +0.8
140,673 - EX28DRAFT_0191 0.11 -0.7

Or see this region's nucleotide sequence