Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0123 and EX28DRAFT_0124 are separated by 1 nucleotides EX28DRAFT_0124 and EX28DRAFT_0125 are separated by 156 nucleotides EX28DRAFT_0125 and EX28DRAFT_0126 are separated by 169 nucleotides
EX28DRAFT_0123: EX28DRAFT_0123 - Large extracellular alpha-helical protein, at 61,574 to 66,526
_0123
EX28DRAFT_0124: EX28DRAFT_0124 - penicillin-binding protein 1C, at 66,528 to 68,852
_0124
EX28DRAFT_0125: EX28DRAFT_0125 - nucleoside diphosphate kinase (EC 2.7.4.6), at 69,009 to 69,440
_0125
EX28DRAFT_0126: EX28DRAFT_0126 - 23S rRNA (adenine(2503)-C(2))-methyltransferase, at 69,610 to 70,776
_0126
Position (kb) 66
67
68
69 Strain fitness (log2 ratio) -2
-1
0
1
2 at 65.760 kb on - strand, within EX28DRAFT_0123 at 65.760 kb on - strand, within EX28DRAFT_0123 at 65.760 kb on - strand, within EX28DRAFT_0123 at 65.785 kb on + strand, within EX28DRAFT_0123 at 65.785 kb on + strand, within EX28DRAFT_0123 at 65.785 kb on + strand, within EX28DRAFT_0123 at 65.941 kb on + strand, within EX28DRAFT_0123 at 66.118 kb on + strand at 66.119 kb on - strand at 66.119 kb on - strand at 66.404 kb on + strand at 66.434 kb on + strand at 66.463 kb on + strand at 66.463 kb on + strand at 66.463 kb on + strand at 66.463 kb on + strand at 66.464 kb on - strand at 66.464 kb on - strand at 66.464 kb on - strand at 66.464 kb on - strand at 66.464 kb on - strand at 66.464 kb on - strand at 66.464 kb on - strand at 66.464 kb on - strand at 66.464 kb on - strand at 66.467 kb on + strand at 66.468 kb on - strand at 66.809 kb on - strand, within EX28DRAFT_0124 at 67.006 kb on - strand, within EX28DRAFT_0124 at 67.066 kb on - strand, within EX28DRAFT_0124 at 67.090 kb on + strand, within EX28DRAFT_0124 at 67.090 kb on + strand, within EX28DRAFT_0124 at 67.090 kb on + strand, within EX28DRAFT_0124 at 67.090 kb on + strand, within EX28DRAFT_0124 at 67.091 kb on - strand, within EX28DRAFT_0124 at 67.091 kb on - strand, within EX28DRAFT_0124 at 67.091 kb on - strand, within EX28DRAFT_0124 at 67.096 kb on - strand, within EX28DRAFT_0124 at 67.144 kb on + strand, within EX28DRAFT_0124 at 67.240 kb on + strand, within EX28DRAFT_0124 at 67.458 kb on - strand, within EX28DRAFT_0124 at 67.491 kb on - strand, within EX28DRAFT_0124 at 67.493 kb on + strand, within EX28DRAFT_0124 at 67.568 kb on + strand, within EX28DRAFT_0124 at 67.577 kb on + strand, within EX28DRAFT_0124 at 67.647 kb on + strand, within EX28DRAFT_0124 at 68.198 kb on - strand, within EX28DRAFT_0124 at 68.198 kb on - strand, within EX28DRAFT_0124 at 68.949 kb on - strand at 68.950 kb on + strand at 68.975 kb on + strand at 69.327 kb on + strand, within EX28DRAFT_0125 at 69.439 kb on - strand at 69.457 kb on - strand at 69.464 kb on - strand at 69.472 kb on - strand at 69.513 kb on + strand at 69.514 kb on - strand at 69.587 kb on + strand at 69.609 kb on + strand at 69.610 kb on - strand at 69.620 kb on + strand at 69.620 kb on + strand at 69.628 kb on - strand at 69.718 kb on - strand at 69.770 kb on - strand, within EX28DRAFT_0126 at 69.775 kb on + strand, within EX28DRAFT_0126
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 65,760 - EX28DRAFT_0123 0.85 +0.2 65,760 - EX28DRAFT_0123 0.85 +0.3 65,760 - EX28DRAFT_0123 0.85 -0.2 65,785 + EX28DRAFT_0123 0.85 +2.6 65,785 + EX28DRAFT_0123 0.85 -0.0 65,785 + EX28DRAFT_0123 0.85 +0.1 65,941 + EX28DRAFT_0123 0.88 -0.1 66,118 + -0.4 66,119 - +0.1 66,119 - -0.1 66,404 + -0.9 66,434 + +0.0 66,463 + -2.0 66,463 + -1.7 66,463 + +0.8 66,463 + -1.3 66,464 - +0.6 66,464 - -1.0 66,464 - +1.7 66,464 - +1.4 66,464 - -0.3 66,464 - +1.9 66,464 - +1.0 66,464 - +0.1 66,464 - -0.3 66,467 + +0.3 66,468 - -0.0 66,809 - EX28DRAFT_0124 0.12 -0.3 67,006 - EX28DRAFT_0124 0.21 +2.1 67,066 - EX28DRAFT_0124 0.23 +0.3 67,090 + EX28DRAFT_0124 0.24 +0.3 67,090 + EX28DRAFT_0124 0.24 +0.1 67,090 + EX28DRAFT_0124 0.24 +0.3 67,090 + EX28DRAFT_0124 0.24 -0.2 67,091 - EX28DRAFT_0124 0.24 +1.1 67,091 - EX28DRAFT_0124 0.24 +0.1 67,091 - EX28DRAFT_0124 0.24 -0.6 67,096 - EX28DRAFT_0124 0.24 +0.1 67,144 + EX28DRAFT_0124 0.26 -0.3 67,240 + EX28DRAFT_0124 0.31 -1.3 67,458 - EX28DRAFT_0124 0.40 -2.3 67,491 - EX28DRAFT_0124 0.41 -0.3 67,493 + EX28DRAFT_0124 0.42 +1.9 67,568 + EX28DRAFT_0124 0.45 +0.4 67,577 + EX28DRAFT_0124 0.45 +0.3 67,647 + EX28DRAFT_0124 0.48 +0.4 68,198 - EX28DRAFT_0124 0.72 -0.3 68,198 - EX28DRAFT_0124 0.72 +1.7 68,949 - +0.3 68,950 + +0.2 68,975 + -0.3 69,327 + EX28DRAFT_0125 0.74 -2.4 69,439 - -1.3 69,457 - -0.7 69,464 - -1.3 69,472 - -0.3 69,513 + -0.7 69,514 - -0.9 69,587 + +0.4 69,609 + +0.1 69,610 - -0.0 69,620 + +0.3 69,620 + -0.1 69,628 - +1.3 69,718 - +1.0 69,770 - EX28DRAFT_0126 0.14 +0.1 69,775 + EX28DRAFT_0126 0.14 -0.6
Or see this region's nucleotide sequence