Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03705 and CCNA_R0089 are separated by 215 nucleotides CCNA_R0089 and CCNA_03706 are separated by 169 nucleotides CCNA_03706 and CCNA_03707 are separated by 85 nucleotides CCNA_03707 and CCNA_03708 are separated by 13 nucleotides CCNA_03708 and CCNA_03709 are separated by 14 nucleotides
CCNA_03705: CCNA_03705 - hypothetical protein, at 3,873,755 to 3,874,225
_03705
CCNA_R0089: CCNA_R0089 - tRNA-Ala, at 3,874,441 to 3,874,516
_R0089
CCNA_03706: CCNA_03706 - small heat shock protein, at 3,874,686 to 3,875,114
_03706
CCNA_03707: CCNA_03707 - hypothetical protein, at 3,875,200 to 3,875,469
_03707
CCNA_03708: CCNA_03708 - transposase, at 3,875,483 to 3,875,851
_03708
CCNA_03709: CCNA_03709 - transposase, at 3,875,866 to 3,876,276
_03709
Position (kb)
3874
3875
3876 Strain fitness (log2 ratio)
-1
0
1
2 at 3873.787 kb on - strand at 3873.851 kb on + strand, within CCNA_03705 at 3873.883 kb on - strand, within CCNA_03705 at 3873.951 kb on + strand, within CCNA_03705 at 3874.109 kb on + strand, within CCNA_03705 at 3874.117 kb on - strand, within CCNA_03705 at 3874.273 kb on - strand at 3874.584 kb on - strand at 3874.584 kb on - strand at 3874.613 kb on - strand at 3874.615 kb on + strand at 3874.623 kb on - strand at 3874.623 kb on - strand at 3874.736 kb on + strand, within CCNA_03706 at 3874.738 kb on + strand, within CCNA_03706 at 3874.738 kb on + strand, within CCNA_03706 at 3874.738 kb on + strand, within CCNA_03706 at 3874.738 kb on + strand, within CCNA_03706 at 3874.738 kb on + strand, within CCNA_03706 at 3874.746 kb on - strand, within CCNA_03706 at 3874.746 kb on - strand, within CCNA_03706 at 3874.805 kb on + strand, within CCNA_03706 at 3874.830 kb on + strand, within CCNA_03706 at 3874.838 kb on - strand, within CCNA_03706 at 3874.838 kb on - strand, within CCNA_03706 at 3874.936 kb on - strand, within CCNA_03706 at 3874.975 kb on + strand, within CCNA_03706 at 3874.995 kb on - strand, within CCNA_03706 at 3875.011 kb on - strand, within CCNA_03706 at 3875.020 kb on - strand, within CCNA_03706 at 3875.020 kb on - strand, within CCNA_03706 at 3875.026 kb on + strand, within CCNA_03706 at 3875.060 kb on - strand, within CCNA_03706 at 3875.060 kb on - strand, within CCNA_03706 at 3875.111 kb on + strand at 3875.119 kb on - strand at 3875.119 kb on - strand at 3875.140 kb on - strand at 3875.190 kb on - strand at 3875.190 kb on - strand at 3875.212 kb on + strand at 3875.227 kb on + strand, within CCNA_03707 at 3875.227 kb on + strand, within CCNA_03707 at 3875.227 kb on + strand, within CCNA_03707 at 3875.227 kb on + strand, within CCNA_03707 at 3875.235 kb on - strand, within CCNA_03707 at 3875.284 kb on - strand, within CCNA_03707 at 3875.286 kb on + strand, within CCNA_03707 at 3875.294 kb on - strand, within CCNA_03707 at 3875.333 kb on + strand, within CCNA_03707 at 3875.455 kb on + strand at 3875.505 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 3,873,787 - -0.2 3,873,851 + CCNA_03705 0.20 +0.3 3,873,883 - CCNA_03705 0.27 +0.0 3,873,951 + CCNA_03705 0.42 -0.1 3,874,109 + CCNA_03705 0.75 -0.3 3,874,117 - CCNA_03705 0.77 +0.2 3,874,273 - +0.1 3,874,584 - -0.8 3,874,584 - -1.2 3,874,613 - +1.9 3,874,615 + +0.3 3,874,623 - -0.6 3,874,623 - -0.6 3,874,736 + CCNA_03706 0.12 -0.1 3,874,738 + CCNA_03706 0.12 -0.4 3,874,738 + CCNA_03706 0.12 +0.5 3,874,738 + CCNA_03706 0.12 -0.7 3,874,738 + CCNA_03706 0.12 -0.3 3,874,738 + CCNA_03706 0.12 +0.0 3,874,746 - CCNA_03706 0.14 +0.7 3,874,746 - CCNA_03706 0.14 +0.1 3,874,805 + CCNA_03706 0.28 -0.1 3,874,830 + CCNA_03706 0.34 +0.0 3,874,838 - CCNA_03706 0.35 -1.1 3,874,838 - CCNA_03706 0.35 -0.8 3,874,936 - CCNA_03706 0.58 -0.0 3,874,975 + CCNA_03706 0.67 +0.2 3,874,995 - CCNA_03706 0.72 -0.3 3,875,011 - CCNA_03706 0.76 -1.1 3,875,020 - CCNA_03706 0.78 +0.3 3,875,020 - CCNA_03706 0.78 -0.3 3,875,026 + CCNA_03706 0.79 -0.2 3,875,060 - CCNA_03706 0.87 -0.5 3,875,060 - CCNA_03706 0.87 -0.3 3,875,111 + -0.0 3,875,119 - -0.7 3,875,119 - +0.0 3,875,140 - -1.6 3,875,190 - +0.9 3,875,190 - -0.2 3,875,212 + +0.3 3,875,227 + CCNA_03707 0.10 +0.6 3,875,227 + CCNA_03707 0.10 -0.4 3,875,227 + CCNA_03707 0.10 +0.1 3,875,227 + CCNA_03707 0.10 -0.1 3,875,235 - CCNA_03707 0.13 +0.1 3,875,284 - CCNA_03707 0.31 -0.5 3,875,286 + CCNA_03707 0.32 -0.1 3,875,294 - CCNA_03707 0.35 +0.1 3,875,333 + CCNA_03707 0.49 -0.7 3,875,455 + -0.2 3,875,505 - -0.2
Or see this region's nucleotide sequence