Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02012 and CCNA_02013 overlap by 1 nucleotides CCNA_02013 and CCNA_02014 overlap by 4 nucleotides CCNA_02014 and CCNA_02015 are separated by 1 nucleotides
CCNA_02012: CCNA_02012 - metal-dependent hydrolase, at 2,158,098 to 2,159,777
_02012
CCNA_02013: CCNA_02013 - pantothenate kinase/Bvg accessory factor, at 2,159,777 to 2,160,562
_02013
CCNA_02014: CCNA_02014 - Biotin-(acetyl-CoA carboxylase) ligase, at 2,160,559 to 2,161,311
_02014
CCNA_02015: CCNA_02015 - NADH-quinone oxidoreductase chain N, at 2,161,313 to 2,162,773
_02015
Position (kb)
2159
2160
2161 Strain fitness (log2 ratio)
-1
0
1 at 2158.790 kb on - strand, within CCNA_02012 at 2158.832 kb on - strand, within CCNA_02012 at 2158.942 kb on + strand, within CCNA_02012 at 2158.988 kb on - strand, within CCNA_02012 at 2159.022 kb on + strand, within CCNA_02012 at 2159.022 kb on + strand, within CCNA_02012 at 2159.022 kb on + strand, within CCNA_02012 at 2159.030 kb on - strand, within CCNA_02012 at 2159.030 kb on - strand, within CCNA_02012 at 2159.030 kb on - strand, within CCNA_02012 at 2159.052 kb on - strand, within CCNA_02012 at 2159.052 kb on - strand, within CCNA_02012 at 2159.054 kb on - strand, within CCNA_02012 at 2159.054 kb on - strand, within CCNA_02012 at 2159.109 kb on - strand, within CCNA_02012 at 2159.109 kb on - strand, within CCNA_02012 at 2159.225 kb on - strand, within CCNA_02012 at 2159.268 kb on - strand, within CCNA_02012 at 2159.268 kb on - strand, within CCNA_02012 at 2159.283 kb on + strand, within CCNA_02012 at 2159.283 kb on + strand, within CCNA_02012 at 2159.291 kb on - strand, within CCNA_02012 at 2159.291 kb on - strand, within CCNA_02012 at 2159.400 kb on - strand, within CCNA_02012 at 2159.493 kb on + strand, within CCNA_02012 at 2159.504 kb on + strand, within CCNA_02012 at 2159.521 kb on + strand, within CCNA_02012 at 2159.536 kb on - strand, within CCNA_02012 at 2159.547 kb on + strand, within CCNA_02012 at 2159.702 kb on - strand at 2161.317 kb on - strand at 2161.321 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,158,790 - CCNA_02012 0.41 +1.2 2,158,832 - CCNA_02012 0.44 +0.8 2,158,942 + CCNA_02012 0.50 +0.8 2,158,988 - CCNA_02012 0.53 -0.4 2,159,022 + CCNA_02012 0.55 -0.9 2,159,022 + CCNA_02012 0.55 -1.1 2,159,022 + CCNA_02012 0.55 +0.2 2,159,030 - CCNA_02012 0.55 +0.3 2,159,030 - CCNA_02012 0.55 +0.7 2,159,030 - CCNA_02012 0.55 +0.4 2,159,052 - CCNA_02012 0.57 +0.4 2,159,052 - CCNA_02012 0.57 -0.8 2,159,054 - CCNA_02012 0.57 +0.3 2,159,054 - CCNA_02012 0.57 -0.4 2,159,109 - CCNA_02012 0.60 -0.0 2,159,109 - CCNA_02012 0.60 -0.6 2,159,225 - CCNA_02012 0.67 +0.7 2,159,268 - CCNA_02012 0.70 +0.8 2,159,268 - CCNA_02012 0.70 +0.0 2,159,283 + CCNA_02012 0.71 -0.4 2,159,283 + CCNA_02012 0.71 +0.8 2,159,291 - CCNA_02012 0.71 +0.7 2,159,291 - CCNA_02012 0.71 +0.6 2,159,400 - CCNA_02012 0.78 +0.1 2,159,493 + CCNA_02012 0.83 -0.2 2,159,504 + CCNA_02012 0.84 -0.7 2,159,521 + CCNA_02012 0.85 -1.6 2,159,536 - CCNA_02012 0.86 +1.1 2,159,547 + CCNA_02012 0.86 -0.8 2,159,702 - -0.1 2,161,317 - +0.6 2,161,321 + -1.1
Or see this region's nucleotide sequence