Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00699 and CCNA_00700 are separated by 718 nucleotides CCNA_00700 and CCNA_00701 are separated by 586 nucleotides CCNA_00701 and CCNA_03888 are separated by 144 nucleotides
CCNA_00699: CCNA_00699 - LSU ribosomal protein L28P, at 758,081 to 758,380
_00699
CCNA_00700: CCNA_00700 - TonB-dependent receptor, at 759,099 to 761,024
_00700
CCNA_00701: CCNA_00701 - cold shock protein CspB, at 761,611 to 761,820
_00701
CCNA_03888: CCNA_03888 - hypothetical protein, at 761,965 to 762,324
_03888
Position (kb) 759
760
761
762 Strain fitness (log2 ratio) -2
-1
0
1
2 at 758.353 kb on + strand at 758.375 kb on + strand at 758.495 kb on + strand at 758.495 kb on + strand at 758.503 kb on - strand at 758.526 kb on - strand at 758.533 kb on + strand at 758.541 kb on - strand at 758.541 kb on - strand at 758.572 kb on - strand at 759.065 kb on + strand at 759.067 kb on + strand at 759.075 kb on + strand at 759.077 kb on + strand at 759.077 kb on + strand at 759.077 kb on + strand at 759.085 kb on - strand at 759.085 kb on - strand at 759.112 kb on + strand at 759.112 kb on + strand at 759.112 kb on + strand at 759.197 kb on - strand at 759.213 kb on + strand at 759.213 kb on + strand at 759.218 kb on + strand at 759.226 kb on + strand at 759.226 kb on + strand at 759.283 kb on + strand at 759.289 kb on - strand at 759.292 kb on + strand, within CCNA_00700 at 759.300 kb on - strand, within CCNA_00700 at 759.300 kb on - strand, within CCNA_00700 at 759.339 kb on + strand, within CCNA_00700 at 759.339 kb on + strand, within CCNA_00700 at 759.347 kb on - strand, within CCNA_00700 at 759.347 kb on - strand, within CCNA_00700 at 759.347 kb on - strand, within CCNA_00700 at 759.347 kb on - strand, within CCNA_00700 at 759.347 kb on - strand, within CCNA_00700 at 759.352 kb on + strand, within CCNA_00700 at 759.360 kb on - strand, within CCNA_00700 at 759.360 kb on - strand, within CCNA_00700 at 759.399 kb on + strand, within CCNA_00700 at 759.424 kb on + strand, within CCNA_00700 at 759.465 kb on + strand, within CCNA_00700 at 759.465 kb on + strand, within CCNA_00700 at 759.489 kb on + strand, within CCNA_00700 at 759.489 kb on + strand, within CCNA_00700 at 759.494 kb on + strand, within CCNA_00700 at 759.494 kb on + strand, within CCNA_00700 at 759.522 kb on + strand, within CCNA_00700 at 759.525 kb on - strand, within CCNA_00700 at 759.573 kb on - strand, within CCNA_00700 at 759.638 kb on + strand, within CCNA_00700 at 759.646 kb on - strand, within CCNA_00700 at 759.646 kb on - strand, within CCNA_00700 at 759.671 kb on - strand, within CCNA_00700 at 759.702 kb on + strand, within CCNA_00700 at 759.734 kb on + strand, within CCNA_00700 at 759.752 kb on + strand, within CCNA_00700 at 759.754 kb on + strand, within CCNA_00700 at 759.756 kb on + strand, within CCNA_00700 at 759.764 kb on - strand, within CCNA_00700 at 759.764 kb on - strand, within CCNA_00700 at 759.784 kb on + strand, within CCNA_00700 at 759.787 kb on - strand, within CCNA_00700 at 759.792 kb on - strand, within CCNA_00700 at 759.792 kb on - strand, within CCNA_00700 at 759.807 kb on - strand, within CCNA_00700 at 759.827 kb on - strand, within CCNA_00700 at 759.867 kb on - strand, within CCNA_00700 at 759.867 kb on - strand, within CCNA_00700 at 759.867 kb on - strand, within CCNA_00700 at 759.872 kb on - strand, within CCNA_00700 at 759.882 kb on - strand, within CCNA_00700 at 759.920 kb on + strand, within CCNA_00700 at 759.920 kb on + strand, within CCNA_00700 at 759.920 kb on + strand, within CCNA_00700 at 759.929 kb on - strand, within CCNA_00700 at 759.997 kb on + strand, within CCNA_00700 at 760.040 kb on - strand, within CCNA_00700 at 760.051 kb on + strand, within CCNA_00700 at 760.059 kb on - strand, within CCNA_00700 at 760.126 kb on + strand, within CCNA_00700 at 760.131 kb on + strand, within CCNA_00700 at 760.167 kb on - strand, within CCNA_00700 at 760.172 kb on + strand, within CCNA_00700 at 760.174 kb on - strand, within CCNA_00700 at 760.236 kb on + strand, within CCNA_00700 at 760.243 kb on + strand, within CCNA_00700 at 760.244 kb on - strand, within CCNA_00700 at 760.244 kb on - strand, within CCNA_00700 at 760.248 kb on + strand, within CCNA_00700 at 760.251 kb on - strand, within CCNA_00700 at 760.258 kb on + strand, within CCNA_00700 at 760.258 kb on + strand, within CCNA_00700 at 760.258 kb on + strand, within CCNA_00700 at 760.276 kb on - strand, within CCNA_00700 at 760.280 kb on + strand, within CCNA_00700 at 760.282 kb on - strand, within CCNA_00700 at 760.421 kb on + strand, within CCNA_00700 at 760.424 kb on - strand, within CCNA_00700 at 760.490 kb on - strand, within CCNA_00700 at 760.505 kb on + strand, within CCNA_00700 at 760.513 kb on - strand, within CCNA_00700 at 760.515 kb on + strand, within CCNA_00700 at 760.515 kb on + strand, within CCNA_00700 at 760.539 kb on + strand, within CCNA_00700 at 760.551 kb on + strand, within CCNA_00700 at 760.575 kb on - strand, within CCNA_00700 at 760.585 kb on - strand, within CCNA_00700 at 760.622 kb on - strand, within CCNA_00700 at 760.715 kb on - strand, within CCNA_00700 at 760.723 kb on + strand, within CCNA_00700 at 760.738 kb on + strand, within CCNA_00700 at 760.803 kb on + strand, within CCNA_00700 at 760.849 kb on - strand at 760.864 kb on + strand at 760.867 kb on + strand at 760.869 kb on + strand at 760.869 kb on + strand at 760.869 kb on + strand at 760.869 kb on + strand at 760.869 kb on + strand at 760.877 kb on - strand at 760.877 kb on - strand at 760.877 kb on - strand at 760.877 kb on - strand at 760.877 kb on - strand at 760.877 kb on - strand at 760.877 kb on - strand at 760.877 kb on - strand at 760.877 kb on - strand at 760.877 kb on - strand at 760.877 kb on - strand at 760.919 kb on - strand at 760.950 kb on + strand at 760.953 kb on - strand at 760.953 kb on - strand at 760.958 kb on - strand at 761.028 kb on - strand at 761.033 kb on - strand at 761.042 kb on + strand at 761.043 kb on + strand at 761.050 kb on - strand at 761.050 kb on - strand at 761.068 kb on + strand at 761.076 kb on - strand at 761.076 kb on - strand at 761.076 kb on - strand at 761.076 kb on - strand at 761.076 kb on - strand at 761.081 kb on + strand at 761.143 kb on - strand at 761.146 kb on + strand at 761.159 kb on - strand at 761.188 kb on + strand at 761.231 kb on + strand at 761.231 kb on - strand at 761.231 kb on - strand at 761.253 kb on - strand at 761.267 kb on + strand at 761.536 kb on + strand at 761.572 kb on + strand at 761.572 kb on + strand at 761.580 kb on - strand at 761.580 kb on - strand at 761.624 kb on + strand at 761.652 kb on - strand, within CCNA_00701 at 761.662 kb on + strand, within CCNA_00701 at 761.717 kb on + strand, within CCNA_00701 at 761.718 kb on - strand, within CCNA_00701 at 761.722 kb on + strand, within CCNA_00701 at 761.722 kb on + strand, within CCNA_00701 at 761.751 kb on - strand, within CCNA_00701 at 761.805 kb on + strand at 761.839 kb on - strand at 761.844 kb on - strand at 761.934 kb on - strand at 761.958 kb on - strand at 761.967 kb on - strand at 761.967 kb on - strand at 761.968 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 758,353 + +0.5 758,375 + +0.4 758,495 + +0.2 758,495 + +0.2 758,503 - -0.5 758,526 - +0.4 758,533 + +0.3 758,541 - +0.2 758,541 - +0.1 758,572 - +1.0 759,065 + +0.0 759,067 + +0.3 759,075 + +0.8 759,077 + -0.6 759,077 + -0.4 759,077 + -0.1 759,085 - +0.2 759,085 - -0.9 759,112 + -0.2 759,112 + +0.2 759,112 + +0.1 759,197 - +0.4 759,213 + -0.5 759,213 + +0.8 759,218 + -0.5 759,226 + +0.4 759,226 + +0.3 759,283 + -0.4 759,289 - -1.3 759,292 + CCNA_00700 0.10 +0.5 759,300 - CCNA_00700 0.10 +0.3 759,300 - CCNA_00700 0.10 -0.1 759,339 + CCNA_00700 0.12 +0.6 759,339 + CCNA_00700 0.12 +0.2 759,347 - CCNA_00700 0.13 +0.1 759,347 - CCNA_00700 0.13 -0.3 759,347 - CCNA_00700 0.13 -0.4 759,347 - CCNA_00700 0.13 -0.7 759,347 - CCNA_00700 0.13 -0.9 759,352 + CCNA_00700 0.13 +0.4 759,360 - CCNA_00700 0.14 +0.8 759,360 - CCNA_00700 0.14 -0.2 759,399 + CCNA_00700 0.16 +0.8 759,424 + CCNA_00700 0.17 -0.1 759,465 + CCNA_00700 0.19 +0.2 759,465 + CCNA_00700 0.19 -1.0 759,489 + CCNA_00700 0.20 -1.6 759,489 + CCNA_00700 0.20 -0.2 759,494 + CCNA_00700 0.21 +0.4 759,494 + CCNA_00700 0.21 -0.3 759,522 + CCNA_00700 0.22 -0.0 759,525 - CCNA_00700 0.22 +0.1 759,573 - CCNA_00700 0.25 -0.5 759,638 + CCNA_00700 0.28 +0.9 759,646 - CCNA_00700 0.28 +0.2 759,646 - CCNA_00700 0.28 +0.8 759,671 - CCNA_00700 0.30 +0.2 759,702 + CCNA_00700 0.31 -0.4 759,734 + CCNA_00700 0.33 +1.8 759,752 + CCNA_00700 0.34 +0.3 759,754 + CCNA_00700 0.34 -0.7 759,756 + CCNA_00700 0.34 +0.9 759,764 - CCNA_00700 0.35 -0.1 759,764 - CCNA_00700 0.35 +0.3 759,784 + CCNA_00700 0.36 -0.2 759,787 - CCNA_00700 0.36 -0.3 759,792 - CCNA_00700 0.36 +2.4 759,792 - CCNA_00700 0.36 -0.5 759,807 - CCNA_00700 0.37 +0.3 759,827 - CCNA_00700 0.38 -0.2 759,867 - CCNA_00700 0.40 -0.5 759,867 - CCNA_00700 0.40 -1.1 759,867 - CCNA_00700 0.40 -0.2 759,872 - CCNA_00700 0.40 +0.4 759,882 - CCNA_00700 0.41 +0.5 759,920 + CCNA_00700 0.43 +0.5 759,920 + CCNA_00700 0.43 +0.1 759,920 + CCNA_00700 0.43 +0.4 759,929 - CCNA_00700 0.43 -0.2 759,997 + CCNA_00700 0.47 +0.2 760,040 - CCNA_00700 0.49 +0.0 760,051 + CCNA_00700 0.49 -0.7 760,059 - CCNA_00700 0.50 -0.0 760,126 + CCNA_00700 0.53 +0.6 760,131 + CCNA_00700 0.54 -0.6 760,167 - CCNA_00700 0.55 +2.0 760,172 + CCNA_00700 0.56 -0.6 760,174 - CCNA_00700 0.56 -1.0 760,236 + CCNA_00700 0.59 +0.2 760,243 + CCNA_00700 0.59 +1.2 760,244 - CCNA_00700 0.59 +0.0 760,244 - CCNA_00700 0.59 +0.3 760,248 + CCNA_00700 0.60 +0.4 760,251 - CCNA_00700 0.60 -1.8 760,258 + CCNA_00700 0.60 +0.5 760,258 + CCNA_00700 0.60 +0.1 760,258 + CCNA_00700 0.60 +0.2 760,276 - CCNA_00700 0.61 -0.3 760,280 + CCNA_00700 0.61 +0.8 760,282 - CCNA_00700 0.61 -0.2 760,421 + CCNA_00700 0.69 +0.4 760,424 - CCNA_00700 0.69 +0.8 760,490 - CCNA_00700 0.72 -0.4 760,505 + CCNA_00700 0.73 -0.2 760,513 - CCNA_00700 0.73 -2.1 760,515 + CCNA_00700 0.74 +0.3 760,515 + CCNA_00700 0.74 -0.2 760,539 + CCNA_00700 0.75 +0.1 760,551 + CCNA_00700 0.75 +0.1 760,575 - CCNA_00700 0.77 +0.1 760,585 - CCNA_00700 0.77 +1.1 760,622 - CCNA_00700 0.79 -0.3 760,715 - CCNA_00700 0.84 +1.0 760,723 + CCNA_00700 0.84 +0.2 760,738 + CCNA_00700 0.85 +0.0 760,803 + CCNA_00700 0.88 +0.2 760,849 - +0.2 760,864 + +0.2 760,867 + +0.1 760,869 + +0.1 760,869 + -0.3 760,869 + +0.1 760,869 + -1.1 760,869 + -0.3 760,877 - -0.2 760,877 - -0.3 760,877 - +0.1 760,877 - -0.9 760,877 - +0.4 760,877 - -0.4 760,877 - +1.0 760,877 - +0.3 760,877 - -0.5 760,877 - +0.3 760,877 - -0.3 760,919 - -0.0 760,950 + +0.4 760,953 - -0.8 760,953 - +0.7 760,958 - +0.5 761,028 - +0.0 761,033 - +0.0 761,042 + -0.2 761,043 + +0.3 761,050 - +1.8 761,050 - +0.0 761,068 + -0.4 761,076 - +0.2 761,076 - -0.2 761,076 - +0.5 761,076 - +0.8 761,076 - +0.0 761,081 + -0.5 761,143 - +0.0 761,146 + -0.2 761,159 - +2.0 761,188 + +1.0 761,231 + +0.2 761,231 - -0.4 761,231 - +1.1 761,253 - -0.1 761,267 + -0.2 761,536 + -0.1 761,572 + +1.4 761,572 + -0.3 761,580 - +0.8 761,580 - +0.7 761,624 + +2.0 761,652 - CCNA_00701 0.20 -0.4 761,662 + CCNA_00701 0.24 -0.8 761,717 + CCNA_00701 0.50 +1.0 761,718 - CCNA_00701 0.51 -0.8 761,722 + CCNA_00701 0.53 -0.9 761,722 + CCNA_00701 0.53 -0.6 761,751 - CCNA_00701 0.67 -0.2 761,805 + +0.6 761,839 - +0.7 761,844 - -0.5 761,934 - +0.5 761,958 - -0.8 761,967 - +1.2 761,967 - -0.6 761,968 + -0.2
Or see this region's nucleotide sequence