Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00475 and CCNA_00476 overlap by 4 nucleotides CCNA_00476 and CCNA_00477 are separated by 380 nucleotides CCNA_00477 and CCNA_00478 are separated by 121 nucleotides
CCNA_00475: CCNA_00475 - bacterial conjugation protein, ATP binding domain trwB, at 491,512 to 493,329
_00475
CCNA_00476: CCNA_00476 - hypothetical protein, at 493,326 to 493,709
_00476
CCNA_00477: CCNA_00477 - excisionase, at 494,090 to 494,284
_00477
CCNA_00478: CCNA_00478 - hypothetical protein, at 494,406 to 495,143
_00478
Position (kb) 493
494 Strain fitness (log2 ratio) -2
-1
0
1
2 at 492.342 kb on + strand, within CCNA_00475 at 492.350 kb on - strand, within CCNA_00475 at 492.364 kb on + strand, within CCNA_00475 at 492.380 kb on + strand, within CCNA_00475 at 492.388 kb on - strand, within CCNA_00475 at 492.388 kb on - strand, within CCNA_00475 at 492.388 kb on - strand, within CCNA_00475 at 492.389 kb on - strand, within CCNA_00475 at 492.421 kb on - strand, within CCNA_00475 at 492.425 kb on + strand, within CCNA_00475 at 492.431 kb on - strand, within CCNA_00475 at 492.431 kb on - strand, within CCNA_00475 at 492.432 kb on + strand, within CCNA_00475 at 492.443 kb on + strand, within CCNA_00475 at 492.465 kb on - strand, within CCNA_00475 at 492.488 kb on - strand, within CCNA_00475 at 492.523 kb on + strand, within CCNA_00475 at 492.532 kb on + strand, within CCNA_00475 at 492.537 kb on + strand, within CCNA_00475 at 492.624 kb on + strand, within CCNA_00475 at 492.626 kb on - strand, within CCNA_00475 at 492.659 kb on + strand, within CCNA_00475 at 492.659 kb on + strand, within CCNA_00475 at 492.746 kb on - strand, within CCNA_00475 at 492.769 kb on - strand, within CCNA_00475 at 492.802 kb on - strand, within CCNA_00475 at 492.814 kb on + strand, within CCNA_00475 at 492.817 kb on - strand, within CCNA_00475 at 492.819 kb on + strand, within CCNA_00475 at 492.819 kb on + strand, within CCNA_00475 at 492.819 kb on + strand, within CCNA_00475 at 492.819 kb on + strand, within CCNA_00475 at 492.819 kb on + strand, within CCNA_00475 at 492.819 kb on + strand, within CCNA_00475 at 492.819 kb on + strand, within CCNA_00475 at 492.827 kb on - strand, within CCNA_00475 at 492.827 kb on - strand, within CCNA_00475 at 492.827 kb on - strand, within CCNA_00475 at 492.827 kb on - strand, within CCNA_00475 at 492.834 kb on + strand, within CCNA_00475 at 492.872 kb on - strand, within CCNA_00475 at 492.907 kb on - strand, within CCNA_00475 at 492.938 kb on + strand, within CCNA_00475 at 492.944 kb on + strand, within CCNA_00475 at 492.946 kb on - strand, within CCNA_00475 at 492.950 kb on + strand, within CCNA_00475 at 492.960 kb on + strand, within CCNA_00475 at 492.960 kb on + strand, within CCNA_00475 at 493.051 kb on - strand, within CCNA_00475 at 493.053 kb on + strand, within CCNA_00475 at 493.054 kb on + strand, within CCNA_00475 at 493.056 kb on + strand, within CCNA_00475 at 493.056 kb on - strand, within CCNA_00475 at 493.062 kb on - strand, within CCNA_00475 at 493.062 kb on - strand, within CCNA_00475 at 493.071 kb on + strand, within CCNA_00475 at 493.071 kb on + strand, within CCNA_00475 at 493.071 kb on + strand, within CCNA_00475 at 493.071 kb on + strand, within CCNA_00475 at 493.071 kb on + strand, within CCNA_00475 at 493.079 kb on - strand, within CCNA_00475 at 493.079 kb on - strand, within CCNA_00475 at 493.079 kb on - strand, within CCNA_00475 at 493.079 kb on - strand, within CCNA_00475 at 493.079 kb on - strand, within CCNA_00475 at 493.079 kb on - strand, within CCNA_00475 at 493.093 kb on + strand, within CCNA_00475 at 493.128 kb on + strand, within CCNA_00475 at 493.139 kb on + strand, within CCNA_00475 at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.156 kb on + strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.164 kb on - strand at 493.173 kb on + strand at 493.173 kb on + strand at 493.173 kb on + strand at 493.173 kb on + strand at 493.191 kb on + strand at 493.191 kb on + strand at 493.234 kb on + strand at 493.234 kb on + strand at 493.242 kb on - strand at 493.265 kb on + strand at 493.270 kb on + strand at 493.270 kb on + strand at 493.270 kb on + strand at 493.270 kb on + strand at 493.277 kb on - strand at 493.278 kb on - strand at 493.278 kb on - strand at 493.278 kb on - strand at 493.278 kb on - strand at 493.282 kb on + strand at 493.290 kb on - strand at 493.314 kb on + strand at 493.356 kb on - strand at 493.362 kb on - strand at 493.429 kb on + strand, within CCNA_00476 at 493.429 kb on + strand, within CCNA_00476 at 493.446 kb on + strand, within CCNA_00476 at 493.446 kb on + strand, within CCNA_00476 at 493.446 kb on + strand, within CCNA_00476 at 493.446 kb on + strand, within CCNA_00476 at 493.454 kb on - strand, within CCNA_00476 at 493.462 kb on + strand, within CCNA_00476 at 493.512 kb on - strand, within CCNA_00476 at 493.520 kb on + strand, within CCNA_00476 at 493.528 kb on - strand, within CCNA_00476 at 493.653 kb on + strand, within CCNA_00476 at 493.661 kb on - strand, within CCNA_00476 at 493.734 kb on - strand at 493.790 kb on + strand at 493.790 kb on + strand at 493.842 kb on + strand at 493.858 kb on + strand at 493.858 kb on + strand at 493.858 kb on + strand at 493.858 kb on + strand at 493.866 kb on - strand at 493.866 kb on - strand at 493.866 kb on - strand at 493.903 kb on + strand at 493.937 kb on + strand at 493.937 kb on + strand at 493.937 kb on + strand at 493.951 kb on + strand at 493.951 kb on + strand at 493.951 kb on + strand at 493.959 kb on - strand at 493.959 kb on - strand at 493.959 kb on - strand at 493.959 kb on - strand at 493.959 kb on - strand at 493.960 kb on + strand at 493.961 kb on - strand at 493.968 kb on - strand at 493.968 kb on - strand at 493.988 kb on + strand at 493.995 kb on + strand at 493.995 kb on + strand at 493.995 kb on + strand at 493.995 kb on + strand at 494.003 kb on - strand at 494.003 kb on - strand at 494.003 kb on - strand at 494.008 kb on - strand at 494.038 kb on + strand at 494.052 kb on + strand at 494.131 kb on - strand, within CCNA_00477 at 494.131 kb on - strand, within CCNA_00477 at 494.135 kb on + strand, within CCNA_00477 at 494.136 kb on - strand, within CCNA_00477 at 494.143 kb on - strand, within CCNA_00477 at 494.151 kb on - strand, within CCNA_00477 at 494.181 kb on + strand, within CCNA_00477 at 494.181 kb on + strand, within CCNA_00477 at 494.181 kb on + strand, within CCNA_00477 at 494.183 kb on + strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.189 kb on - strand, within CCNA_00477 at 494.193 kb on + strand, within CCNA_00477 at 494.198 kb on + strand, within CCNA_00477 at 494.201 kb on - strand, within CCNA_00477 at 494.217 kb on + strand, within CCNA_00477 at 494.222 kb on + strand, within CCNA_00477 at 494.223 kb on + strand, within CCNA_00477 at 494.231 kb on - strand, within CCNA_00477 at 494.231 kb on - strand, within CCNA_00477 at 494.233 kb on + strand, within CCNA_00477 at 494.243 kb on + strand, within CCNA_00477 at 494.243 kb on + strand, within CCNA_00477 at 494.251 kb on - strand, within CCNA_00477 at 494.266 kb on - strand at 494.275 kb on - strand at 494.292 kb on + strand at 494.292 kb on + strand at 494.300 kb on - strand at 494.313 kb on + strand at 494.313 kb on + strand at 494.313 kb on + strand at 494.313 kb on + strand at 494.313 kb on + strand at 494.321 kb on - strand at 494.321 kb on - strand at 494.348 kb on + strand at 494.370 kb on + strand at 494.373 kb on - strand at 494.378 kb on - strand at 494.384 kb on + strand at 494.403 kb on + strand at 494.411 kb on - strand at 494.411 kb on - strand at 494.428 kb on + strand at 494.428 kb on + strand at 494.428 kb on + strand at 494.428 kb on + strand at 494.428 kb on + strand at 494.436 kb on - strand at 494.448 kb on + strand at 494.453 kb on - strand at 494.464 kb on - strand at 494.491 kb on - strand, within CCNA_00478 at 494.506 kb on - strand, within CCNA_00478 at 494.540 kb on + strand, within CCNA_00478 at 494.572 kb on + strand, within CCNA_00478 at 494.593 kb on + strand, within CCNA_00478 at 494.594 kb on + strand, within CCNA_00478 at 494.602 kb on - strand, within CCNA_00478 at 494.605 kb on + strand, within CCNA_00478 at 494.616 kb on + strand, within CCNA_00478 at 494.616 kb on + strand, within CCNA_00478 at 494.616 kb on + strand, within CCNA_00478 at 494.624 kb on - strand, within CCNA_00478 at 494.624 kb on - strand, within CCNA_00478 at 494.624 kb on - strand, within CCNA_00478 at 494.639 kb on - strand, within CCNA_00478 at 494.646 kb on - strand, within CCNA_00478 at 494.674 kb on - strand, within CCNA_00478 at 494.676 kb on + strand, within CCNA_00478 at 494.676 kb on + strand, within CCNA_00478 at 494.685 kb on - strand, within CCNA_00478 at 494.703 kb on - strand, within CCNA_00478
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 492,342 + CCNA_00475 0.46 -0.3 492,350 - CCNA_00475 0.46 -0.2 492,364 + CCNA_00475 0.47 -2.5 492,380 + CCNA_00475 0.48 +0.1 492,388 - CCNA_00475 0.48 -0.1 492,388 - CCNA_00475 0.48 -0.1 492,388 - CCNA_00475 0.48 -0.9 492,389 - CCNA_00475 0.48 -0.2 492,421 - CCNA_00475 0.50 -0.4 492,425 + CCNA_00475 0.50 -0.5 492,431 - CCNA_00475 0.51 +0.0 492,431 - CCNA_00475 0.51 -0.4 492,432 + CCNA_00475 0.51 +0.4 492,443 + CCNA_00475 0.51 +0.7 492,465 - CCNA_00475 0.52 +0.3 492,488 - CCNA_00475 0.54 -0.2 492,523 + CCNA_00475 0.56 -0.4 492,532 + CCNA_00475 0.56 -0.9 492,537 + CCNA_00475 0.56 +0.7 492,624 + CCNA_00475 0.61 +0.4 492,626 - CCNA_00475 0.61 -1.6 492,659 + CCNA_00475 0.63 -0.4 492,659 + CCNA_00475 0.63 -2.7 492,746 - CCNA_00475 0.68 -2.3 492,769 - CCNA_00475 0.69 -1.8 492,802 - CCNA_00475 0.71 +0.1 492,814 + CCNA_00475 0.72 -0.7 492,817 - CCNA_00475 0.72 -0.5 492,819 + CCNA_00475 0.72 +1.1 492,819 + CCNA_00475 0.72 -0.2 492,819 + CCNA_00475 0.72 -0.1 492,819 + CCNA_00475 0.72 -0.4 492,819 + CCNA_00475 0.72 +0.7 492,819 + CCNA_00475 0.72 -0.6 492,819 + CCNA_00475 0.72 -1.3 492,827 - CCNA_00475 0.72 -1.7 492,827 - CCNA_00475 0.72 -0.3 492,827 - CCNA_00475 0.72 +0.2 492,827 - CCNA_00475 0.72 -0.6 492,834 + CCNA_00475 0.73 +1.2 492,872 - CCNA_00475 0.75 -0.1 492,907 - CCNA_00475 0.77 -2.2 492,938 + CCNA_00475 0.78 -1.1 492,944 + CCNA_00475 0.79 +0.1 492,946 - CCNA_00475 0.79 +0.5 492,950 + CCNA_00475 0.79 -0.3 492,960 + CCNA_00475 0.80 -0.8 492,960 + CCNA_00475 0.80 -0.5 493,051 - CCNA_00475 0.85 -0.7 493,053 + CCNA_00475 0.85 +1.0 493,054 + CCNA_00475 0.85 -0.5 493,056 + CCNA_00475 0.85 -0.8 493,056 - CCNA_00475 0.85 -1.1 493,062 - CCNA_00475 0.85 +0.3 493,062 - CCNA_00475 0.85 -0.6 493,071 + CCNA_00475 0.86 +0.3 493,071 + CCNA_00475 0.86 +0.5 493,071 + CCNA_00475 0.86 -0.3 493,071 + CCNA_00475 0.86 -0.8 493,071 + CCNA_00475 0.86 +0.2 493,079 - CCNA_00475 0.86 -0.3 493,079 - CCNA_00475 0.86 +0.2 493,079 - CCNA_00475 0.86 -0.3 493,079 - CCNA_00475 0.86 -2.2 493,079 - CCNA_00475 0.86 -0.7 493,079 - CCNA_00475 0.86 -0.7 493,093 + CCNA_00475 0.87 -0.9 493,128 + CCNA_00475 0.89 -0.6 493,139 + CCNA_00475 0.89 -2.1 493,156 + -1.1 493,156 + +0.0 493,156 + +0.6 493,156 + -0.9 493,156 + -0.9 493,156 + -0.3 493,156 + -0.8 493,156 + +0.4 493,156 + +0.1 493,156 + -0.7 493,156 + -1.6 493,156 + +0.3 493,156 + +0.4 493,156 + -0.3 493,156 + -0.7 493,156 + -1.3 493,156 + -0.7 493,164 - -0.7 493,164 - +0.4 493,164 - +0.1 493,164 - +0.1 493,164 - -0.1 493,164 - +1.1 493,164 - -0.5 493,164 - -0.7 493,164 - +0.4 493,164 - -1.5 493,164 - -0.9 493,164 - +0.5 493,164 - -1.0 493,164 - -0.8 493,164 - -0.3 493,164 - -1.2 493,164 - -1.0 493,164 - -0.2 493,164 - +0.4 493,173 + +0.1 493,173 + -0.3 493,173 + -0.9 493,173 + -0.0 493,191 + -0.1 493,191 + -1.8 493,234 + -0.4 493,234 + +0.4 493,242 - -0.6 493,265 + -0.2 493,270 + -0.0 493,270 + -0.3 493,270 + -0.6 493,270 + -0.4 493,277 - +1.1 493,278 - -0.5 493,278 - +0.2 493,278 - -2.1 493,278 - +0.3 493,282 + -0.2 493,290 - -0.6 493,314 + -0.9 493,356 - -2.4 493,362 - -0.3 493,429 + CCNA_00476 0.27 -1.0 493,429 + CCNA_00476 0.27 -0.3 493,446 + CCNA_00476 0.31 +0.2 493,446 + CCNA_00476 0.31 +0.2 493,446 + CCNA_00476 0.31 -0.3 493,446 + CCNA_00476 0.31 -0.2 493,454 - CCNA_00476 0.33 +0.6 493,462 + CCNA_00476 0.35 +0.1 493,512 - CCNA_00476 0.48 -0.1 493,520 + CCNA_00476 0.51 -0.9 493,528 - CCNA_00476 0.53 -1.2 493,653 + CCNA_00476 0.85 -1.0 493,661 - CCNA_00476 0.87 +0.4 493,734 - -0.1 493,790 + -1.4 493,790 + -0.3 493,842 + -0.1 493,858 + -0.4 493,858 + -1.3 493,858 + +0.7 493,858 + -0.9 493,866 - +0.9 493,866 - +0.4 493,866 - +1.7 493,903 + -1.7 493,937 + -1.9 493,937 + -0.6 493,937 + -1.6 493,951 + -0.5 493,951 + +0.1 493,951 + -0.1 493,959 - -0.5 493,959 - -1.2 493,959 - +1.1 493,959 - -2.1 493,959 - +0.4 493,960 + -0.4 493,961 - -0.7 493,968 - -1.9 493,968 - +0.5 493,988 + +0.6 493,995 + -0.0 493,995 + -0.0 493,995 + +0.3 493,995 + -0.6 494,003 - +0.8 494,003 - -0.9 494,003 - -1.0 494,008 - -2.1 494,038 + -0.0 494,052 + -2.3 494,131 - CCNA_00477 0.21 +1.9 494,131 - CCNA_00477 0.21 -0.6 494,135 + CCNA_00477 0.23 +0.0 494,136 - CCNA_00477 0.24 -0.3 494,143 - CCNA_00477 0.27 +0.3 494,151 - CCNA_00477 0.31 -0.4 494,181 + CCNA_00477 0.47 +0.2 494,181 + CCNA_00477 0.47 -0.9 494,181 + CCNA_00477 0.47 -1.5 494,183 + CCNA_00477 0.48 -0.0 494,189 - CCNA_00477 0.51 +0.1 494,189 - CCNA_00477 0.51 -0.0 494,189 - CCNA_00477 0.51 +0.0 494,189 - CCNA_00477 0.51 -1.6 494,193 + CCNA_00477 0.53 -0.0 494,198 + CCNA_00477 0.55 -2.1 494,201 - CCNA_00477 0.57 -1.2 494,217 + CCNA_00477 0.65 +0.0 494,222 + CCNA_00477 0.68 +1.3 494,223 + CCNA_00477 0.68 +0.2 494,231 - CCNA_00477 0.72 +0.7 494,231 - CCNA_00477 0.72 +0.7 494,233 + CCNA_00477 0.73 +0.5 494,243 + CCNA_00477 0.78 +0.1 494,243 + CCNA_00477 0.78 +0.3 494,251 - CCNA_00477 0.83 +0.1 494,266 - +0.5 494,275 - -0.3 494,292 + -0.2 494,292 + -1.1 494,300 - +0.1 494,313 + -0.3 494,313 + -0.4 494,313 + +0.4 494,313 + +0.1 494,313 + +0.2 494,321 - +0.1 494,321 - -0.5 494,348 + +0.1 494,370 + -0.7 494,373 - +0.6 494,378 - +0.7 494,384 + +0.7 494,403 + -0.0 494,411 - -1.7 494,411 - -0.1 494,428 + -1.3 494,428 + -0.9 494,428 + -0.3 494,428 + -0.3 494,428 + +0.0 494,436 - +0.2 494,448 + -0.2 494,453 - +0.3 494,464 - -0.3 494,491 - CCNA_00478 0.12 +0.2 494,506 - CCNA_00478 0.14 -0.2 494,540 + CCNA_00478 0.18 -0.3 494,572 + CCNA_00478 0.22 -1.2 494,593 + CCNA_00478 0.25 -0.7 494,594 + CCNA_00478 0.25 +0.4 494,602 - CCNA_00478 0.27 -0.0 494,605 + CCNA_00478 0.27 +0.0 494,616 + CCNA_00478 0.28 -0.3 494,616 + CCNA_00478 0.28 +0.6 494,616 + CCNA_00478 0.28 +0.0 494,624 - CCNA_00478 0.30 -0.6 494,624 - CCNA_00478 0.30 +0.1 494,624 - CCNA_00478 0.30 -0.2 494,639 - CCNA_00478 0.32 +0.5 494,646 - CCNA_00478 0.33 -0.1 494,674 - CCNA_00478 0.36 -0.2 494,676 + CCNA_00478 0.37 -1.8 494,676 + CCNA_00478 0.37 -0.4 494,685 - CCNA_00478 0.38 +0.7 494,703 - CCNA_00478 0.40 -0.3
Or see this region's nucleotide sequence