Experiment: 30_mag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS22760 and AMB_RS22765 overlap by 4 nucleotides AMB_RS22765 and AMB_RS22770 are separated by 85 nucleotides AMB_RS22770 and AMB_RS22775 are separated by 1 nucleotides
AMB_RS22760: AMB_RS22760 - ribonuclease PH, at 4,913,102 to 4,913,821
_RS22760
AMB_RS22765: AMB_RS22765 - non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family, at 4,913,818 to 4,914,423
_RS22765
AMB_RS22770: AMB_RS22770 - radical SAM protein, at 4,914,509 to 4,915,636
_RS22770
AMB_RS22775: AMB_RS22775 - penicillin-binding protein activator, at 4,915,638 to 4,917,008
_RS22775
Position (kb) 4914
4915
4916 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4913.600 kb on + strand, within AMB_RS22760 at 4913.600 kb on + strand, within AMB_RS22760 at 4914.088 kb on + strand, within AMB_RS22765 at 4914.292 kb on + strand, within AMB_RS22765 at 4914.533 kb on + strand at 4914.533 kb on + strand at 4914.534 kb on - strand at 4914.534 kb on - strand at 4914.534 kb on - strand at 4914.570 kb on - strand at 4915.271 kb on + strand, within AMB_RS22770 at 4915.272 kb on - strand, within AMB_RS22770 at 4915.272 kb on - strand, within AMB_RS22770 at 4915.272 kb on - strand, within AMB_RS22770 at 4915.639 kb on + strand at 4915.846 kb on + strand, within AMB_RS22775 at 4916.048 kb on - strand, within AMB_RS22775 at 4916.048 kb on - strand, within AMB_RS22775 at 4916.204 kb on - strand, within AMB_RS22775 at 4916.204 kb on - strand, within AMB_RS22775
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_1 remove 4,913,600 + AMB_RS22760 0.69 -0.2 4,913,600 + AMB_RS22760 0.69 -0.6 4,914,088 + AMB_RS22765 0.45 -0.5 4,914,292 + AMB_RS22765 0.78 +0.4 4,914,533 + -0.3 4,914,533 + +0.3 4,914,534 - -3.0 4,914,534 - -0.8 4,914,534 - -1.9 4,914,570 - -0.2 4,915,271 + AMB_RS22770 0.68 -0.5 4,915,272 - AMB_RS22770 0.68 -0.5 4,915,272 - AMB_RS22770 0.68 -0.5 4,915,272 - AMB_RS22770 0.68 -0.7 4,915,639 + -0.1 4,915,846 + AMB_RS22775 0.15 +2.5 4,916,048 - AMB_RS22775 0.30 -0.8 4,916,048 - AMB_RS22775 0.30 +3.2 4,916,204 - AMB_RS22775 0.41 -0.1 4,916,204 - AMB_RS22775 0.41 -1.2
Or see this region's nucleotide sequence