Experiment: 30_mag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS21475 and AMB_RS21480 overlap by 8 nucleotides AMB_RS21480 and AMB_RS21485 overlap by 4 nucleotides AMB_RS21485 and AMB_RS21490 are separated by 62 nucleotides AMB_RS21490 and AMB_RS21495 are separated by 23 nucleotides
AMB_RS21475: AMB_RS21475 - aminoglycoside phosphotransferase, at 4,657,902 to 4,658,894
_RS21475
AMB_RS21480: AMB_RS21480 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 4,658,887 to 4,659,357
_RS21480
AMB_RS21485: AMB_RS21485 - tyrosine recombinase XerC, at 4,659,354 to 4,660,298
_RS21485
AMB_RS21490: AMB_RS21490 - DUF805 domain-containing protein, at 4,660,361 to 4,660,726
_RS21490
AMB_RS21495: AMB_RS21495 - DUF484 domain-containing protein, at 4,660,750 to 4,661,463
_RS21495
Position (kb) 4659
4660
4661 Strain fitness (log2 ratio) -1
0
1
2 at 4659.815 kb on - strand, within AMB_RS21485 at 4660.363 kb on - strand at 4660.651 kb on - strand, within AMB_RS21490 at 4660.687 kb on - strand, within AMB_RS21490 at 4660.687 kb on - strand, within AMB_RS21490 at 4660.691 kb on - strand at 4660.727 kb on - strand at 4660.727 kb on - strand at 4660.752 kb on - strand at 4661.209 kb on - strand, within AMB_RS21495 at 4661.209 kb on - strand, within AMB_RS21495
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_1 remove 4,659,815 - AMB_RS21485 0.49 -0.7 4,660,363 - -1.6 4,660,651 - AMB_RS21490 0.79 -0.0 4,660,687 - AMB_RS21490 0.89 -0.7 4,660,687 - AMB_RS21490 0.89 -0.1 4,660,691 - -1.4 4,660,727 - +0.2 4,660,727 - +2.2 4,660,752 - +0.1 4,661,209 - AMB_RS21495 0.64 +0.2 4,661,209 - AMB_RS21495 0.64 +0.1
Or see this region's nucleotide sequence