Experiment: 30_mag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04255 and AMB_RS04260 are separated by 4 nucleotides AMB_RS04260 and AMB_RS04265 are separated by 4 nucleotides AMB_RS04265 and AMB_RS04270 are separated by 79 nucleotides
AMB_RS04255: AMB_RS04255 - isoleucine--tRNA ligase, at 892,564 to 895,409
_RS04255
AMB_RS04260: AMB_RS04260 - signal peptidase II, at 895,414 to 895,932
_RS04260
AMB_RS04265: AMB_RS04265 - DUF3035 domain-containing protein, at 895,937 to 896,524
_RS04265
AMB_RS04270: AMB_RS04270 - insulinase family protein, at 896,604 to 898,001
_RS04270
Position (kb) 895
896
897 Strain fitness (log2 ratio) -2
-1
0
1 at 895.923 kb on + strand at 895.930 kb on + strand at 896.523 kb on - strand at 896.600 kb on + strand at 896.601 kb on - strand at 896.769 kb on + strand, within AMB_RS04270 at 897.123 kb on + strand, within AMB_RS04270 at 897.123 kb on + strand, within AMB_RS04270 at 897.124 kb on - strand, within AMB_RS04270 at 897.295 kb on - strand, within AMB_RS04270 at 897.295 kb on - strand, within AMB_RS04270 at 897.298 kb on - strand, within AMB_RS04270 at 897.420 kb on + strand, within AMB_RS04270 at 897.463 kb on - strand, within AMB_RS04270 at 897.516 kb on + strand, within AMB_RS04270 at 897.517 kb on - strand, within AMB_RS04270
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_1 remove 895,923 + -1.3 895,930 + -2.3 896,523 - +0.1 896,600 + +0.4 896,601 - -0.1 896,769 + AMB_RS04270 0.12 +0.5 897,123 + AMB_RS04270 0.37 -0.2 897,123 + AMB_RS04270 0.37 +1.1 897,124 - AMB_RS04270 0.37 -0.1 897,295 - AMB_RS04270 0.49 -1.5 897,295 - AMB_RS04270 0.49 +0.2 897,298 - AMB_RS04270 0.50 -0.5 897,420 + AMB_RS04270 0.58 +1.3 897,463 - AMB_RS04270 0.61 -0.2 897,516 + AMB_RS04270 0.65 -0.3 897,517 - AMB_RS04270 0.65 +0.2
Or see this region's nucleotide sequence