Experiment: 30_mag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01230 and AMB_RS01235 overlap by 4 nucleotides AMB_RS01235 and AMB_RS01240 are separated by 101 nucleotides AMB_RS01240 and AMB_RS01245 are separated by 317 nucleotides
AMB_RS01230: AMB_RS01230 - methionyl-tRNA formyltransferase, at 274,510 to 275,427
_RS01230
AMB_RS01235: AMB_RS01235 - peptide deformylase, at 275,424 to 275,936
_RS01235
AMB_RS01240: AMB_RS01240 - 16S rRNA (uracil(1498)-N(3))-methyltransferase, at 276,038 to 276,796
_RS01240
AMB_RS01245: AMB_RS01245 - methyl-accepting chemotaxis protein, at 277,114 to 278,799
_RS01245
Position (kb) 276
277 Strain fitness (log2 ratio) -2
-1
0
1 at 275.973 kb on - strand at 276.134 kb on + strand, within AMB_RS01240 at 276.134 kb on + strand, within AMB_RS01240 at 276.134 kb on + strand, within AMB_RS01240 at 276.134 kb on + strand, within AMB_RS01240 at 276.134 kb on + strand, within AMB_RS01240 at 276.134 kb on + strand, within AMB_RS01240 at 276.135 kb on - strand, within AMB_RS01240 at 276.135 kb on - strand, within AMB_RS01240 at 276.135 kb on - strand, within AMB_RS01240 at 276.135 kb on - strand, within AMB_RS01240 at 276.135 kb on - strand, within AMB_RS01240 at 276.794 kb on + strand at 276.795 kb on - strand at 276.795 kb on - strand at 276.795 kb on - strand at 276.919 kb on - strand at 276.983 kb on - strand at 277.012 kb on + strand at 277.045 kb on + strand at 277.241 kb on - strand at 277.241 kb on - strand at 277.264 kb on + strand at 277.297 kb on + strand, within AMB_RS01245 at 277.297 kb on + strand, within AMB_RS01245 at 277.297 kb on + strand, within AMB_RS01245 at 277.298 kb on - strand, within AMB_RS01245 at 277.298 kb on - strand, within AMB_RS01245 at 277.298 kb on - strand, within AMB_RS01245 at 277.298 kb on - strand, within AMB_RS01245 at 277.313 kb on - strand, within AMB_RS01245 at 277.313 kb on - strand, within AMB_RS01245 at 277.397 kb on - strand, within AMB_RS01245 at 277.397 kb on - strand, within AMB_RS01245 at 277.414 kb on + strand, within AMB_RS01245 at 277.414 kb on + strand, within AMB_RS01245 at 277.415 kb on - strand, within AMB_RS01245 at 277.471 kb on + strand, within AMB_RS01245 at 277.471 kb on + strand, within AMB_RS01245 at 277.471 kb on + strand, within AMB_RS01245 at 277.472 kb on - strand, within AMB_RS01245 at 277.513 kb on + strand, within AMB_RS01245 at 277.513 kb on + strand, within AMB_RS01245 at 277.513 kb on + strand, within AMB_RS01245 at 277.582 kb on + strand, within AMB_RS01245 at 277.583 kb on - strand, within AMB_RS01245 at 277.616 kb on + strand, within AMB_RS01245 at 277.617 kb on - strand, within AMB_RS01245 at 277.678 kb on + strand, within AMB_RS01245 at 277.678 kb on + strand, within AMB_RS01245 at 277.678 kb on + strand, within AMB_RS01245 at 277.679 kb on - strand, within AMB_RS01245
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_1 remove 275,973 - +0.2 276,134 + AMB_RS01240 0.13 -0.6 276,134 + AMB_RS01240 0.13 -0.0 276,134 + AMB_RS01240 0.13 -0.6 276,134 + AMB_RS01240 0.13 +0.4 276,134 + AMB_RS01240 0.13 -0.1 276,134 + AMB_RS01240 0.13 -1.7 276,135 - AMB_RS01240 0.13 -0.0 276,135 - AMB_RS01240 0.13 +0.5 276,135 - AMB_RS01240 0.13 -0.4 276,135 - AMB_RS01240 0.13 -0.2 276,135 - AMB_RS01240 0.13 -1.3 276,794 + -0.0 276,795 - -0.2 276,795 - -0.2 276,795 - +0.4 276,919 - -0.8 276,983 - +0.0 277,012 + +0.6 277,045 + -0.3 277,241 - +0.3 277,241 - +0.4 277,264 + -0.7 277,297 + AMB_RS01245 0.11 -0.2 277,297 + AMB_RS01245 0.11 +0.1 277,297 + AMB_RS01245 0.11 +1.0 277,298 - AMB_RS01245 0.11 -0.8 277,298 - AMB_RS01245 0.11 -1.2 277,298 - AMB_RS01245 0.11 -2.0 277,298 - AMB_RS01245 0.11 +0.4 277,313 - AMB_RS01245 0.12 -0.8 277,313 - AMB_RS01245 0.12 +0.3 277,397 - AMB_RS01245 0.17 +0.3 277,397 - AMB_RS01245 0.17 +0.2 277,414 + AMB_RS01245 0.18 -1.5 277,414 + AMB_RS01245 0.18 -0.3 277,415 - AMB_RS01245 0.18 -0.6 277,471 + AMB_RS01245 0.21 -0.5 277,471 + AMB_RS01245 0.21 +1.0 277,471 + AMB_RS01245 0.21 +0.9 277,472 - AMB_RS01245 0.21 -0.2 277,513 + AMB_RS01245 0.24 -0.3 277,513 + AMB_RS01245 0.24 +0.3 277,513 + AMB_RS01245 0.24 +1.0 277,582 + AMB_RS01245 0.28 -0.5 277,583 - AMB_RS01245 0.28 +0.2 277,616 + AMB_RS01245 0.30 -0.4 277,617 - AMB_RS01245 0.30 -0.1 277,678 + AMB_RS01245 0.33 +0.7 277,678 + AMB_RS01245 0.33 -0.3 277,678 + AMB_RS01245 0.33 -1.5 277,679 - AMB_RS01245 0.34 -0.3
Or see this region's nucleotide sequence