Experiment: L-Methionine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS20425 and ABIE40_RS20430 are separated by 1 nucleotides ABIE40_RS20430 and ABIE40_RS20435 overlap by 4 nucleotides ABIE40_RS20435 and ABIE40_RS20440 overlap by 2 nucleotides ABIE40_RS20440 and ABIE40_RS20445 overlap by 37 nucleotides
ABIE40_RS20425: ABIE40_RS20425 - TIM barrel protein, at 189,628 to 190,440
_RS20425
ABIE40_RS20430: ABIE40_RS20430 - precorrin-4 C(11)-methyltransferase, at 190,442 to 191,200
_RS20430
ABIE40_RS20435: ABIE40_RS20435 - precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE, at 191,197 to 192,435
_RS20435
ABIE40_RS20440: ABIE40_RS20440 - cobalt-precorrin-6A reductase, at 192,434 to 193,204
_RS20440
ABIE40_RS20445: ABIE40_RS20445 - precorrin-3B C(17)-methyltransferase, at 193,168 to 193,932
_RS20445
Position (kb) 191
192
193 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 190.333 kb on + strand, within ABIE40_RS20425 at 190.333 kb on + strand, within ABIE40_RS20425 at 190.333 kb on + strand, within ABIE40_RS20425 at 190.334 kb on - strand, within ABIE40_RS20425 at 190.420 kb on + strand at 190.420 kb on + strand at 190.421 kb on - strand at 190.421 kb on - strand at 190.421 kb on - strand at 190.421 kb on - strand at 190.443 kb on + strand at 190.444 kb on - strand at 190.470 kb on + strand at 190.579 kb on + strand, within ABIE40_RS20430 at 191.004 kb on + strand, within ABIE40_RS20430 at 191.005 kb on - strand, within ABIE40_RS20430 at 191.005 kb on - strand, within ABIE40_RS20430 at 191.070 kb on + strand, within ABIE40_RS20430 at 191.071 kb on - strand, within ABIE40_RS20430 at 191.076 kb on - strand, within ABIE40_RS20430 at 191.076 kb on - strand, within ABIE40_RS20430 at 191.193 kb on - strand at 191.193 kb on - strand at 191.193 kb on - strand at 191.663 kb on + strand, within ABIE40_RS20435 at 191.733 kb on - strand, within ABIE40_RS20435 at 191.811 kb on + strand, within ABIE40_RS20435 at 191.893 kb on + strand, within ABIE40_RS20435 at 191.894 kb on - strand, within ABIE40_RS20435 at 191.921 kb on + strand, within ABIE40_RS20435 at 192.165 kb on - strand, within ABIE40_RS20435 at 192.456 kb on + strand at 192.456 kb on + strand at 192.456 kb on + strand at 192.521 kb on - strand, within ABIE40_RS20440 at 193.052 kb on + strand, within ABIE40_RS20440 at 193.052 kb on + strand, within ABIE40_RS20440 at 193.053 kb on - strand, within ABIE40_RS20440 at 193.053 kb on - strand, within ABIE40_RS20440 at 193.201 kb on - strand at 193.201 kb on - strand at 193.318 kb on - strand, within ABIE40_RS20445
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (C) remove 190,333 + ABIE40_RS20425 0.87 +0.1 190,333 + ABIE40_RS20425 0.87 +0.1 190,333 + ABIE40_RS20425 0.87 -0.4 190,334 - ABIE40_RS20425 0.87 -0.1 190,420 + +1.1 190,420 + +0.4 190,421 - +1.3 190,421 - +0.1 190,421 - -1.7 190,421 - -0.5 190,443 + -0.3 190,444 - -1.1 190,470 + -1.3 190,579 + ABIE40_RS20430 0.18 -0.9 191,004 + ABIE40_RS20430 0.74 -1.1 191,005 - ABIE40_RS20430 0.74 -2.9 191,005 - ABIE40_RS20430 0.74 -2.6 191,070 + ABIE40_RS20430 0.83 -1.5 191,071 - ABIE40_RS20430 0.83 +1.2 191,076 - ABIE40_RS20430 0.84 -1.6 191,076 - ABIE40_RS20430 0.84 -1.2 191,193 - -1.3 191,193 - -2.9 191,193 - -2.5 191,663 + ABIE40_RS20435 0.38 -1.6 191,733 - ABIE40_RS20435 0.43 -2.5 191,811 + ABIE40_RS20435 0.50 -1.6 191,893 + ABIE40_RS20435 0.56 -1.8 191,894 - ABIE40_RS20435 0.56 -2.1 191,921 + ABIE40_RS20435 0.58 -1.6 192,165 - ABIE40_RS20435 0.78 -1.3 192,456 + -0.6 192,456 + +0.2 192,456 + -0.8 192,521 - ABIE40_RS20440 0.11 -2.3 193,052 + ABIE40_RS20440 0.80 -2.2 193,052 + ABIE40_RS20440 0.80 -1.3 193,053 - ABIE40_RS20440 0.80 -0.3 193,053 - ABIE40_RS20440 0.80 -1.8 193,201 - -1.6 193,201 - -2.4 193,318 - ABIE40_RS20445 0.20 -1.8
Or see this region's nucleotide sequence