Experiment: L-Methionine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS01915 and ABIE40_RS01920 overlap by 23 nucleotides ABIE40_RS01920 and ABIE40_RS01925 overlap by 13 nucleotides ABIE40_RS01925 and ABIE40_RS01930 overlap by 14 nucleotides ABIE40_RS01930 and ABIE40_RS01935 are separated by 110 nucleotides
ABIE40_RS01915: ABIE40_RS01915 - tRNA pseudouridine(38-40) synthase TruA, at 378,089 to 378,895
_RS01915
ABIE40_RS01920: ABIE40_RS01920 - arsenate reductase (glutaredoxin), at 378,873 to 379,289
_RS01920
ABIE40_RS01925: ABIE40_RS01925 - hypothetical protein, at 379,277 to 379,873
_RS01925
ABIE40_RS01930: ABIE40_RS01930 - succinyl-diaminopimelate desuccinylase, at 379,860 to 381,053
_RS01930
ABIE40_RS01935: ABIE40_RS01935 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 381,164 to 382,024
_RS01935
Position (kb) 379
380
381
382 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 378.888 kb on + strand at 378.888 kb on + strand at 378.888 kb on + strand at 378.889 kb on - strand at 378.889 kb on - strand at 378.889 kb on - strand at 378.889 kb on - strand at 378.889 kb on - strand at 378.970 kb on - strand, within ABIE40_RS01920 at 378.970 kb on - strand, within ABIE40_RS01920 at 378.970 kb on - strand, within ABIE40_RS01920 at 379.085 kb on + strand, within ABIE40_RS01920 at 379.086 kb on - strand, within ABIE40_RS01920 at 379.086 kb on - strand, within ABIE40_RS01920 at 379.287 kb on + strand at 379.288 kb on - strand at 379.311 kb on + strand at 379.311 kb on + strand at 379.509 kb on + strand, within ABIE40_RS01925 at 379.509 kb on + strand, within ABIE40_RS01925 at 379.509 kb on + strand, within ABIE40_RS01925 at 379.651 kb on - strand, within ABIE40_RS01925 at 379.671 kb on + strand, within ABIE40_RS01925 at 379.671 kb on + strand, within ABIE40_RS01925 at 379.672 kb on - strand, within ABIE40_RS01925 at 379.672 kb on - strand, within ABIE40_RS01925 at 379.672 kb on - strand, within ABIE40_RS01925 at 379.845 kb on + strand at 379.845 kb on + strand at 379.845 kb on + strand at 379.845 kb on + strand at 379.845 kb on + strand at 379.846 kb on - strand at 379.846 kb on - strand at 379.846 kb on - strand at 379.848 kb on + strand at 379.848 kb on + strand at 379.848 kb on + strand at 379.849 kb on - strand at 379.849 kb on - strand at 379.861 kb on + strand at 379.862 kb on - strand at 379.862 kb on - strand at 379.903 kb on + strand at 379.904 kb on - strand at 379.904 kb on - strand at 379.955 kb on + strand at 379.955 kb on + strand at 379.955 kb on + strand at 379.956 kb on - strand at 379.956 kb on - strand at 380.447 kb on - strand, within ABIE40_RS01930 at 380.835 kb on - strand, within ABIE40_RS01930 at 380.882 kb on - strand, within ABIE40_RS01930 at 381.065 kb on + strand at 381.165 kb on + strand at 381.165 kb on + strand at 381.165 kb on + strand at 381.166 kb on - strand at 381.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (C) remove 378,888 + +0.8 378,888 + -0.2 378,888 + -0.1 378,889 - +0.4 378,889 - -0.2 378,889 - -0.7 378,889 - -1.3 378,889 - -0.4 378,970 - ABIE40_RS01920 0.23 -0.6 378,970 - ABIE40_RS01920 0.23 -0.5 378,970 - ABIE40_RS01920 0.23 -0.7 379,085 + ABIE40_RS01920 0.51 -1.2 379,086 - ABIE40_RS01920 0.51 +0.2 379,086 - ABIE40_RS01920 0.51 -0.5 379,287 + +0.1 379,288 - -0.6 379,311 + -1.0 379,311 + +0.3 379,509 + ABIE40_RS01925 0.39 +0.1 379,509 + ABIE40_RS01925 0.39 -0.6 379,509 + ABIE40_RS01925 0.39 -2.1 379,651 - ABIE40_RS01925 0.63 +1.2 379,671 + ABIE40_RS01925 0.66 -1.6 379,671 + ABIE40_RS01925 0.66 +0.2 379,672 - ABIE40_RS01925 0.66 +1.5 379,672 - ABIE40_RS01925 0.66 +0.2 379,672 - ABIE40_RS01925 0.66 -0.5 379,845 + +0.4 379,845 + +1.2 379,845 + -0.8 379,845 + +2.3 379,845 + -0.9 379,846 - -0.0 379,846 - +1.9 379,846 - +0.5 379,848 + +0.5 379,848 + +1.0 379,848 + -2.0 379,849 - -0.8 379,849 - -0.3 379,861 + +0.5 379,862 - +0.3 379,862 - +0.7 379,903 + -2.3 379,904 - -2.9 379,904 - -2.8 379,955 + -1.5 379,955 + -2.1 379,955 + +0.8 379,956 - -2.5 379,956 - -1.8 380,447 - ABIE40_RS01930 0.49 -0.6 380,835 - ABIE40_RS01930 0.82 -3.2 380,882 - ABIE40_RS01930 0.86 -0.8 381,065 + -1.0 381,165 + -1.5 381,165 + +0.5 381,165 + +1.8 381,166 - -0.2 381,166 - +0.1
Or see this region's nucleotide sequence