Experiment: m.b. nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0257 and Echvi_0258 are separated by 5 nucleotides Echvi_0258 and Echvi_0259 are separated by 22 nucleotides Echvi_0259 and Echvi_0260 are separated by 99 nucleotides Echvi_0260 and Echvi_0261 are separated by 168 nucleotides
Echvi_0257: Echvi_0257 - translation elongation factor EF-G, at 259,598 to 261,718
_0257
Echvi_0258: Echvi_0258 - ribosomal protein S7, bacterial/organelle, at 261,724 to 262,191
_0258
Echvi_0259: Echvi_0259 - ribosomal protein S12, bacterial/organelle, at 262,214 to 262,594
_0259
Echvi_0260: Echvi_0260 - Protein of unknown function (DUF3467)., at 262,694 to 263,023
_0260
Echvi_0261: Echvi_0261 - DNA-directed RNA polymerase, beta' subunit, predominant form, at 263,192 to 267,505
_0261
Position (kb) 262
263 Strain fitness (log2 ratio) -1
0
1 at 262.757 kb on + strand, within Echvi_0260 at 262.763 kb on - strand, within Echvi_0260 at 262.840 kb on + strand, within Echvi_0260 at 262.840 kb on + strand, within Echvi_0260 at 262.941 kb on + strand, within Echvi_0260 at 262.941 kb on + strand, within Echvi_0260 at 263.011 kb on + strand at 263.012 kb on - strand at 263.012 kb on - strand at 263.012 kb on - strand at 263.014 kb on + strand at 263.057 kb on - strand at 263.057 kb on - strand at 263.110 kb on + strand at 263.111 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 20 mM remove 262,757 + Echvi_0260 0.19 +0.8 262,763 - Echvi_0260 0.21 -1.0 262,840 + Echvi_0260 0.44 -0.0 262,840 + Echvi_0260 0.44 +1.0 262,941 + Echvi_0260 0.75 +0.3 262,941 + Echvi_0260 0.75 +1.0 263,011 + +0.2 263,012 - -0.7 263,012 - +1.0 263,012 - -0.6 263,014 + +0.1 263,057 - -1.0 263,057 - -0.3 263,110 + +0.3 263,111 - -0.2
Or see this region's nucleotide sequence