Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01981 and CCNA_03960 overlap by 7 nucleotides CCNA_03960 and CCNA_01982 overlap by 2 nucleotides
CCNA_01981: CCNA_01981 - DNA translocation competence protein ComA, at 2,125,014 to 2,127,191
_01981
CCNA_03960: CCNA_03960 - hypothetical protein, at 2,127,185 to 2,127,355
_03960
CCNA_01982: CCNA_01982 - glutamyl-tRNA synthetase, at 2,127,354 to 2,128,766
_01982
Position (kb)
2127
2128 Strain fitness (log2 ratio)
-2
-1
0
1 at 2126.198 kb on - strand, within CCNA_01981 at 2126.198 kb on - strand, within CCNA_01981 at 2126.198 kb on - strand, within CCNA_01981 at 2126.210 kb on - strand, within CCNA_01981 at 2126.264 kb on + strand, within CCNA_01981 at 2126.264 kb on + strand, within CCNA_01981 at 2126.324 kb on - strand, within CCNA_01981 at 2126.351 kb on - strand, within CCNA_01981 at 2126.351 kb on - strand, within CCNA_01981 at 2126.358 kb on - strand, within CCNA_01981 at 2126.373 kb on - strand, within CCNA_01981 at 2126.385 kb on + strand, within CCNA_01981 at 2126.446 kb on - strand, within CCNA_01981 at 2126.526 kb on - strand, within CCNA_01981 at 2126.535 kb on - strand, within CCNA_01981 at 2126.609 kb on - strand, within CCNA_01981 at 2126.704 kb on - strand, within CCNA_01981 at 2126.724 kb on - strand, within CCNA_01981 at 2126.742 kb on + strand, within CCNA_01981 at 2126.788 kb on - strand, within CCNA_01981 at 2126.874 kb on - strand, within CCNA_01981 at 2126.915 kb on - strand, within CCNA_01981 at 2126.944 kb on + strand, within CCNA_01981 at 2127.026 kb on + strand at 2127.027 kb on - strand at 2127.113 kb on + strand at 2127.238 kb on + strand, within CCNA_03960 at 2127.238 kb on + strand, within CCNA_03960 at 2127.342 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 2,126,198 - CCNA_01981 0.54 +0.1 2,126,198 - CCNA_01981 0.54 -0.4 2,126,198 - CCNA_01981 0.54 -0.2 2,126,210 - CCNA_01981 0.55 -0.3 2,126,264 + CCNA_01981 0.57 -0.2 2,126,264 + CCNA_01981 0.57 +1.3 2,126,324 - CCNA_01981 0.60 -0.9 2,126,351 - CCNA_01981 0.61 -1.0 2,126,351 - CCNA_01981 0.61 +0.6 2,126,358 - CCNA_01981 0.62 +0.3 2,126,373 - CCNA_01981 0.62 -1.0 2,126,385 + CCNA_01981 0.63 -0.5 2,126,446 - CCNA_01981 0.66 +0.1 2,126,526 - CCNA_01981 0.69 +0.5 2,126,535 - CCNA_01981 0.70 +0.4 2,126,609 - CCNA_01981 0.73 -0.1 2,126,704 - CCNA_01981 0.78 +0.4 2,126,724 - CCNA_01981 0.79 +0.4 2,126,742 + CCNA_01981 0.79 +1.7 2,126,788 - CCNA_01981 0.81 +0.1 2,126,874 - CCNA_01981 0.85 -0.1 2,126,915 - CCNA_01981 0.87 +0.9 2,126,944 + CCNA_01981 0.89 -0.7 2,127,026 + -1.8 2,127,027 - -0.5 2,127,113 + -0.1 2,127,238 + CCNA_03960 0.31 -0.5 2,127,238 + CCNA_03960 0.31 -0.9 2,127,342 + +0.8
Or see this region's nucleotide sequence