Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03652 and CCNA_03653 are separated by 123 nucleotides CCNA_03653 and CCNA_03910 are separated by 212 nucleotides CCNA_03910 and CCNA_03655 are separated by 85 nucleotides
CCNA_03652: CCNA_03652 - ATP-dependent nuclease subunit A, at 3,811,849 to 3,815,310
_03652
CCNA_03653: CCNA_03653 - thioredoxin, at 3,815,434 to 3,815,766
_03653
CCNA_03910: CCNA_03910 - hypothetical protein, at 3,815,979 to 3,816,086
_03910
CCNA_03655: CCNA_03655 - folylpolyglutamate synthase/dihydrofolate synthase, at 3,816,172 to 3,817,485
_03655
Position (kb)
3815
3816
3817 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3814.991 kb on - strand at 3814.996 kb on - strand at 3815.011 kb on + strand at 3815.027 kb on + strand at 3815.032 kb on + strand at 3815.052 kb on - strand at 3815.181 kb on + strand at 3815.196 kb on - strand at 3815.249 kb on + strand at 3815.257 kb on - strand at 3815.257 kb on - strand at 3815.257 kb on - strand at 3815.305 kb on + strand at 3815.312 kb on - strand at 3815.313 kb on - strand at 3815.336 kb on + strand at 3815.344 kb on - strand at 3815.363 kb on - strand at 3815.422 kb on - strand at 3815.819 kb on - strand at 3815.868 kb on - strand at 3815.873 kb on - strand at 3815.923 kb on + strand at 3815.923 kb on + strand at 3815.924 kb on - strand at 3815.931 kb on - strand at 3815.936 kb on - strand at 3815.975 kb on + strand at 3816.069 kb on + strand, within CCNA_03910 at 3816.070 kb on + strand, within CCNA_03910 at 3816.076 kb on + strand at 3816.084 kb on - strand at 3816.100 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 3,814,991 - +0.0 3,814,996 - -2.0 3,815,011 + -0.6 3,815,027 + +0.5 3,815,032 + +0.4 3,815,052 - -1.4 3,815,181 + -0.6 3,815,196 - -1.1 3,815,249 + +1.0 3,815,257 - -1.8 3,815,257 - -1.6 3,815,257 - -1.0 3,815,305 + -0.5 3,815,312 - -0.4 3,815,313 - -0.1 3,815,336 + -0.2 3,815,344 - -0.3 3,815,363 - -1.2 3,815,422 - +0.0 3,815,819 - +0.5 3,815,868 - +2.3 3,815,873 - +0.6 3,815,923 + +0.3 3,815,923 + +0.1 3,815,924 - +0.3 3,815,931 - +0.2 3,815,936 - +0.3 3,815,975 + -0.0 3,816,069 + CCNA_03910 0.83 -0.9 3,816,070 + CCNA_03910 0.84 -1.7 3,816,076 + -0.3 3,816,084 - +0.8 3,816,100 - +0.3
Or see this region's nucleotide sequence