Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03876 and CCNA_03877 are separated by 258 nucleotides CCNA_03877 and CCNA_03878 overlap by 52 nucleotides CCNA_03878 and CCNA_03879 overlap by 4 nucleotides
CCNA_03876: CCNA_03876 - transcription termination factor rho, at 4,038,433 to 4,039,866
_03876
CCNA_03877: CCNA_03877 - conserved hypothetical membrane spanning protein, at 4,040,125 to 4,040,640
_03877
CCNA_03878: CCNA_03878 - ferrochelatase, at 4,040,589 to 4,041,632
_03878
CCNA_03879: CCNA_03879 - uroporphyrinogen decarboxylase, at 4,041,629 to 4,042,684
_03879
Position (kb)
4040
4041
4042 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4039.966 kb on - strand at 4039.981 kb on + strand at 4040.060 kb on + strand at 4040.068 kb on - strand at 4040.102 kb on + strand at 4040.123 kb on + strand at 4040.602 kb on + strand at 4040.698 kb on + strand, within CCNA_03878 at 4040.698 kb on + strand, within CCNA_03878 at 4040.706 kb on - strand, within CCNA_03878 at 4040.706 kb on - strand, within CCNA_03878 at 4040.706 kb on - strand, within CCNA_03878 at 4040.706 kb on - strand, within CCNA_03878 at 4040.706 kb on - strand, within CCNA_03878 at 4041.648 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 4,039,966 - -0.5 4,039,981 + -0.3 4,040,060 + +0.6 4,040,068 - +0.3 4,040,102 + +0.4 4,040,123 + -0.9 4,040,602 + -0.3 4,040,698 + CCNA_03878 0.10 -0.2 4,040,698 + CCNA_03878 0.10 +0.1 4,040,706 - CCNA_03878 0.11 -1.2 4,040,706 - CCNA_03878 0.11 +0.1 4,040,706 - CCNA_03878 0.11 -0.8 4,040,706 - CCNA_03878 0.11 -3.0 4,040,706 - CCNA_03878 0.11 -0.8 4,041,648 - -0.2
Or see this region's nucleotide sequence