Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02277 and CCNA_02278 are separated by 106 nucleotides CCNA_02278 and CCNA_02279 overlap by 4 nucleotides CCNA_02279 and CCNA_02280 overlap by 4 nucleotides
CCNA_02277: CCNA_02277 - TonB-dependent outer membrane channel, at 2,426,768 to 2,428,969
_02277
CCNA_02278: CCNA_02278 - hypothetical protein, at 2,429,076 to 2,429,684
_02278
CCNA_02279: CCNA_02279 - disulfide bond formation protein B, at 2,429,681 to 2,430,310
_02279
CCNA_02280: CCNA_02280 - 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 2,430,307 to 2,430,867
_02280
Position (kb)
2429
2430 Strain fitness (log2 ratio)
-2
-1
0
1 at 2428.095 kb on + strand, within CCNA_02277 at 2428.275 kb on - strand, within CCNA_02277 at 2428.502 kb on - strand, within CCNA_02277 at 2428.533 kb on + strand, within CCNA_02277 at 2428.597 kb on + strand, within CCNA_02277 at 2428.736 kb on + strand, within CCNA_02277 at 2428.839 kb on + strand at 2428.891 kb on + strand at 2429.623 kb on - strand, within CCNA_02278 at 2429.627 kb on - strand at 2429.695 kb on + strand at 2429.748 kb on - strand, within CCNA_02279 at 2429.748 kb on - strand, within CCNA_02279 at 2429.962 kb on + strand, within CCNA_02279 at 2430.301 kb on - strand at 2430.304 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 2,428,095 + CCNA_02277 0.60 +0.3 2,428,275 - CCNA_02277 0.68 +0.4 2,428,502 - CCNA_02277 0.79 +0.7 2,428,533 + CCNA_02277 0.80 +0.4 2,428,597 + CCNA_02277 0.83 +0.2 2,428,736 + CCNA_02277 0.89 +0.3 2,428,839 + +0.4 2,428,891 + +0.0 2,429,623 - CCNA_02278 0.90 -1.8 2,429,627 - -0.2 2,429,695 + +0.5 2,429,748 - CCNA_02279 0.11 -1.5 2,429,748 - CCNA_02279 0.11 -0.0 2,429,962 + CCNA_02279 0.45 +0.3 2,430,301 - -1.4 2,430,304 + +0.3
Or see this region's nucleotide sequence