Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02009 and CCNA_02010 are separated by 18 nucleotides CCNA_02010 and CCNA_02011 overlap by 1 nucleotides CCNA_02011 and CCNA_02012 are separated by 108 nucleotides CCNA_02012 and CCNA_02013 overlap by 1 nucleotides CCNA_02013 and CCNA_02014 overlap by 4 nucleotides
CCNA_02009: CCNA_02009 - hypothetical protein, at 2,156,875 to 2,157,291
_02009
CCNA_02010: CCNA_02010 - hypothetical protein, at 2,157,310 to 2,157,570
_02010
CCNA_02011: CCNA_02011 - methylmalonyl CoA epimerase, at 2,157,570 to 2,157,989
_02011
CCNA_02012: CCNA_02012 - metal-dependent hydrolase, at 2,158,098 to 2,159,777
_02012
CCNA_02013: CCNA_02013 - pantothenate kinase/Bvg accessory factor, at 2,159,777 to 2,160,562
_02013
CCNA_02014: CCNA_02014 - Biotin-(acetyl-CoA carboxylase) ligase, at 2,160,559 to 2,161,311
_02014
Position (kb)
2158
2159
2160 Strain fitness (log2 ratio)
-2
-1
0
1 at 2157.170 kb on + strand, within CCNA_02009 at 2157.178 kb on - strand, within CCNA_02009 at 2157.203 kb on + strand, within CCNA_02009 at 2157.204 kb on + strand, within CCNA_02009 at 2157.280 kb on + strand at 2157.295 kb on - strand at 2157.300 kb on - strand at 2157.344 kb on + strand, within CCNA_02010 at 2157.344 kb on + strand, within CCNA_02010 at 2157.352 kb on - strand, within CCNA_02010 at 2157.477 kb on - strand, within CCNA_02010 at 2157.640 kb on + strand, within CCNA_02011 at 2157.642 kb on + strand, within CCNA_02011 at 2157.653 kb on - strand, within CCNA_02011 at 2157.655 kb on - strand, within CCNA_02011 at 2157.676 kb on + strand, within CCNA_02011 at 2157.726 kb on + strand, within CCNA_02011 at 2157.729 kb on + strand, within CCNA_02011 at 2157.763 kb on + strand, within CCNA_02011 at 2157.763 kb on + strand, within CCNA_02011 at 2157.763 kb on + strand, within CCNA_02011 at 2157.763 kb on + strand, within CCNA_02011 at 2157.771 kb on - strand, within CCNA_02011 at 2157.799 kb on - strand, within CCNA_02011 at 2157.808 kb on + strand, within CCNA_02011 at 2157.809 kb on - strand, within CCNA_02011 at 2157.822 kb on - strand, within CCNA_02011 at 2157.866 kb on - strand, within CCNA_02011 at 2157.891 kb on - strand, within CCNA_02011 at 2157.902 kb on + strand, within CCNA_02011 at 2157.961 kb on - strand at 2158.032 kb on - strand at 2158.076 kb on - strand at 2158.176 kb on - strand at 2158.183 kb on - strand at 2158.268 kb on - strand, within CCNA_02012 at 2158.314 kb on + strand, within CCNA_02012 at 2158.314 kb on + strand, within CCNA_02012 at 2158.330 kb on - strand, within CCNA_02012 at 2158.431 kb on - strand, within CCNA_02012 at 2158.438 kb on - strand, within CCNA_02012 at 2158.531 kb on - strand, within CCNA_02012 at 2158.533 kb on + strand, within CCNA_02012 at 2158.557 kb on - strand, within CCNA_02012 at 2158.557 kb on - strand, within CCNA_02012 at 2158.723 kb on + strand, within CCNA_02012 at 2158.790 kb on - strand, within CCNA_02012 at 2158.832 kb on - strand, within CCNA_02012 at 2158.942 kb on + strand, within CCNA_02012 at 2158.988 kb on - strand, within CCNA_02012 at 2159.022 kb on + strand, within CCNA_02012 at 2159.022 kb on + strand, within CCNA_02012 at 2159.022 kb on + strand, within CCNA_02012 at 2159.030 kb on - strand, within CCNA_02012 at 2159.030 kb on - strand, within CCNA_02012 at 2159.030 kb on - strand, within CCNA_02012 at 2159.052 kb on - strand, within CCNA_02012 at 2159.052 kb on - strand, within CCNA_02012 at 2159.054 kb on - strand, within CCNA_02012 at 2159.054 kb on - strand, within CCNA_02012 at 2159.109 kb on - strand, within CCNA_02012 at 2159.109 kb on - strand, within CCNA_02012 at 2159.225 kb on - strand, within CCNA_02012 at 2159.268 kb on - strand, within CCNA_02012 at 2159.268 kb on - strand, within CCNA_02012 at 2159.283 kb on + strand, within CCNA_02012 at 2159.283 kb on + strand, within CCNA_02012 at 2159.291 kb on - strand, within CCNA_02012 at 2159.291 kb on - strand, within CCNA_02012 at 2159.400 kb on - strand, within CCNA_02012 at 2159.493 kb on + strand, within CCNA_02012 at 2159.504 kb on + strand, within CCNA_02012 at 2159.521 kb on + strand, within CCNA_02012 at 2159.536 kb on - strand, within CCNA_02012 at 2159.547 kb on + strand, within CCNA_02012 at 2159.702 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 2,157,170 + CCNA_02009 0.71 -1.0 2,157,178 - CCNA_02009 0.73 +0.1 2,157,203 + CCNA_02009 0.79 -0.3 2,157,204 + CCNA_02009 0.79 -0.5 2,157,280 + +0.0 2,157,295 - -0.8 2,157,300 - +0.3 2,157,344 + CCNA_02010 0.13 -1.4 2,157,344 + CCNA_02010 0.13 -1.1 2,157,352 - CCNA_02010 0.16 +0.1 2,157,477 - CCNA_02010 0.64 -0.1 2,157,640 + CCNA_02011 0.17 +0.1 2,157,642 + CCNA_02011 0.17 +0.7 2,157,653 - CCNA_02011 0.20 +0.2 2,157,655 - CCNA_02011 0.20 -1.1 2,157,676 + CCNA_02011 0.25 +1.4 2,157,726 + CCNA_02011 0.37 -2.1 2,157,729 + CCNA_02011 0.38 -1.4 2,157,763 + CCNA_02011 0.46 -0.7 2,157,763 + CCNA_02011 0.46 -0.2 2,157,763 + CCNA_02011 0.46 -0.3 2,157,763 + CCNA_02011 0.46 +0.3 2,157,771 - CCNA_02011 0.48 +1.2 2,157,799 - CCNA_02011 0.55 +0.1 2,157,808 + CCNA_02011 0.57 -0.7 2,157,809 - CCNA_02011 0.57 -0.2 2,157,822 - CCNA_02011 0.60 -0.4 2,157,866 - CCNA_02011 0.70 -0.1 2,157,891 - CCNA_02011 0.76 +0.3 2,157,902 + CCNA_02011 0.79 +0.3 2,157,961 - +1.0 2,158,032 - +1.6 2,158,076 - +0.3 2,158,176 - +0.1 2,158,183 - -0.8 2,158,268 - CCNA_02012 0.10 +0.6 2,158,314 + CCNA_02012 0.13 -1.9 2,158,314 + CCNA_02012 0.13 -1.0 2,158,330 - CCNA_02012 0.14 -0.2 2,158,431 - CCNA_02012 0.20 +1.5 2,158,438 - CCNA_02012 0.20 +0.5 2,158,531 - CCNA_02012 0.26 +0.4 2,158,533 + CCNA_02012 0.26 -0.6 2,158,557 - CCNA_02012 0.27 +0.1 2,158,557 - CCNA_02012 0.27 +0.6 2,158,723 + CCNA_02012 0.37 -0.2 2,158,790 - CCNA_02012 0.41 +0.6 2,158,832 - CCNA_02012 0.44 +0.3 2,158,942 + CCNA_02012 0.50 +1.4 2,158,988 - CCNA_02012 0.53 +0.7 2,159,022 + CCNA_02012 0.55 +0.1 2,159,022 + CCNA_02012 0.55 -0.1 2,159,022 + CCNA_02012 0.55 -0.7 2,159,030 - CCNA_02012 0.55 -0.6 2,159,030 - CCNA_02012 0.55 -0.1 2,159,030 - CCNA_02012 0.55 +0.2 2,159,052 - CCNA_02012 0.57 -0.7 2,159,052 - CCNA_02012 0.57 -0.7 2,159,054 - CCNA_02012 0.57 -0.6 2,159,054 - CCNA_02012 0.57 -0.7 2,159,109 - CCNA_02012 0.60 +0.0 2,159,109 - CCNA_02012 0.60 +0.1 2,159,225 - CCNA_02012 0.67 +1.0 2,159,268 - CCNA_02012 0.70 +1.1 2,159,268 - CCNA_02012 0.70 -0.2 2,159,283 + CCNA_02012 0.71 -0.6 2,159,283 + CCNA_02012 0.71 +0.8 2,159,291 - CCNA_02012 0.71 +1.1 2,159,291 - CCNA_02012 0.71 +0.7 2,159,400 - CCNA_02012 0.78 -0.9 2,159,493 + CCNA_02012 0.83 -0.2 2,159,504 + CCNA_02012 0.84 -0.6 2,159,521 + CCNA_02012 0.85 -0.5 2,159,536 - CCNA_02012 0.86 -0.6 2,159,547 + CCNA_02012 0.86 +0.3 2,159,702 - +0.5
Or see this region's nucleotide sequence