Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01800 and CCNA_01801 are separated by 5 nucleotides CCNA_01801 and CCNA_01802 overlap by 4 nucleotides CCNA_01802 and CCNA_01803 are separated by 12 nucleotides
CCNA_01800: CCNA_01800 - pyruvate dehydrogenase E1 component beta subunit, at 1,924,985 to 1,926,337
_01800
CCNA_01801: CCNA_01801 - hypothetical protein, at 1,926,343 to 1,926,795
_01801
CCNA_01802: CCNA_01802 - hypothetical protein, at 1,926,792 to 1,926,908
_01802
CCNA_01803: CCNA_01803 - pyruvate dehydrogenase complex, dihydrolipoamide acetyltransferase component, at 1,926,921 to 1,928,207
_01803
Position (kb)
1926
1927 Strain fitness (log2 ratio)
-1
0
1 at 1925.912 kb on + strand, within CCNA_01800 at 1925.961 kb on + strand, within CCNA_01800 at 1926.327 kb on + strand at 1926.443 kb on - strand, within CCNA_01801 at 1926.631 kb on + strand, within CCNA_01801 at 1926.631 kb on + strand, within CCNA_01801 at 1926.631 kb on + strand, within CCNA_01801 at 1926.656 kb on + strand, within CCNA_01801 at 1926.702 kb on - strand, within CCNA_01801 at 1926.704 kb on + strand, within CCNA_01801 at 1926.725 kb on + strand, within CCNA_01801 at 1926.733 kb on - strand, within CCNA_01801 at 1926.746 kb on + strand, within CCNA_01801 at 1926.746 kb on - strand, within CCNA_01801 at 1926.784 kb on - strand at 1926.801 kb on + strand at 1926.809 kb on - strand, within CCNA_01802 at 1926.916 kb on + strand at 1927.872 kb on + strand, within CCNA_01803 at 1927.897 kb on + strand, within CCNA_01803
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 1,925,912 + CCNA_01800 0.69 -0.3 1,925,961 + CCNA_01800 0.72 +1.3 1,926,327 + +0.2 1,926,443 - CCNA_01801 0.22 -1.6 1,926,631 + CCNA_01801 0.64 +0.8 1,926,631 + CCNA_01801 0.64 -0.1 1,926,631 + CCNA_01801 0.64 -0.0 1,926,656 + CCNA_01801 0.69 +0.7 1,926,702 - CCNA_01801 0.79 -0.4 1,926,704 + CCNA_01801 0.80 +0.3 1,926,725 + CCNA_01801 0.84 +0.2 1,926,733 - CCNA_01801 0.86 +0.2 1,926,746 + CCNA_01801 0.89 -1.5 1,926,746 - CCNA_01801 0.89 -0.5 1,926,784 - -1.4 1,926,801 + +1.1 1,926,809 - CCNA_01802 0.15 -1.2 1,926,916 + -0.4 1,927,872 + CCNA_01803 0.74 -0.4 1,927,897 + CCNA_01803 0.76 +0.6
Or see this region's nucleotide sequence