Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01637 and CCNA_01638 overlap by 4 nucleotides CCNA_01638 and CCNA_01639 are separated by 111 nucleotides
CCNA_01637: CCNA_01637 - topoisomerase IV subunit A, at 1,755,866 to 1,758,145
_01637
CCNA_01638: CCNA_01638 - beta-lactamase family protein, at 1,758,142 to 1,759,530
_01638
CCNA_01639: CCNA_01639 - beta-lactamase family protein, at 1,759,642 to 1,760,952
_01639
Position (kb)
1758
1759
1760 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1758.234 kb on + strand at 1758.234 kb on + strand at 1758.242 kb on - strand at 1758.242 kb on - strand at 1758.245 kb on + strand at 1758.245 kb on + strand at 1758.245 kb on + strand at 1758.298 kb on + strand, within CCNA_01638 at 1758.367 kb on + strand, within CCNA_01638 at 1758.367 kb on + strand, within CCNA_01638 at 1758.367 kb on + strand, within CCNA_01638 at 1758.367 kb on + strand, within CCNA_01638 at 1758.367 kb on + strand, within CCNA_01638 at 1758.375 kb on - strand, within CCNA_01638 at 1758.375 kb on - strand, within CCNA_01638 at 1758.523 kb on - strand, within CCNA_01638 at 1758.602 kb on + strand, within CCNA_01638 at 1758.625 kb on - strand, within CCNA_01638 at 1758.658 kb on - strand, within CCNA_01638 at 1758.663 kb on - strand, within CCNA_01638 at 1758.699 kb on - strand, within CCNA_01638 at 1758.709 kb on + strand, within CCNA_01638 at 1758.729 kb on + strand, within CCNA_01638 at 1758.748 kb on - strand, within CCNA_01638 at 1758.756 kb on - strand, within CCNA_01638 at 1758.797 kb on - strand, within CCNA_01638 at 1758.816 kb on + strand, within CCNA_01638 at 1758.816 kb on + strand, within CCNA_01638 at 1758.824 kb on - strand, within CCNA_01638 at 1758.824 kb on - strand, within CCNA_01638 at 1758.849 kb on - strand, within CCNA_01638 at 1758.929 kb on + strand, within CCNA_01638 at 1758.937 kb on - strand, within CCNA_01638 at 1758.956 kb on - strand, within CCNA_01638 at 1758.956 kb on - strand, within CCNA_01638 at 1758.996 kb on - strand, within CCNA_01638 at 1759.045 kb on + strand, within CCNA_01638 at 1759.153 kb on + strand, within CCNA_01638 at 1759.456 kb on + strand at 1759.456 kb on + strand at 1759.464 kb on - strand at 1759.464 kb on - strand at 1759.531 kb on + strand at 1759.612 kb on - strand at 1759.709 kb on + strand at 1759.714 kb on + strand at 1759.804 kb on - strand, within CCNA_01639 at 1759.875 kb on - strand, within CCNA_01639 at 1759.875 kb on - strand, within CCNA_01639 at 1759.985 kb on + strand, within CCNA_01639 at 1759.993 kb on - strand, within CCNA_01639 at 1760.028 kb on + strand, within CCNA_01639 at 1760.040 kb on - strand, within CCNA_01639 at 1760.066 kb on - strand, within CCNA_01639 at 1760.181 kb on + strand, within CCNA_01639 at 1760.181 kb on + strand, within CCNA_01639 at 1760.187 kb on + strand, within CCNA_01639 at 1760.195 kb on - strand, within CCNA_01639 at 1760.195 kb on - strand, within CCNA_01639 at 1760.292 kb on - strand, within CCNA_01639 at 1760.385 kb on + strand, within CCNA_01639 at 1760.486 kb on + strand, within CCNA_01639 at 1760.486 kb on + strand, within CCNA_01639 at 1760.494 kb on - strand, within CCNA_01639 at 1760.504 kb on + strand, within CCNA_01639
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 1,758,234 + +1.6 1,758,234 + -2.4 1,758,242 - +0.1 1,758,242 - +0.3 1,758,245 + -3.0 1,758,245 + +1.6 1,758,245 + -1.4 1,758,298 + CCNA_01638 0.11 -0.9 1,758,367 + CCNA_01638 0.16 -1.2 1,758,367 + CCNA_01638 0.16 +0.1 1,758,367 + CCNA_01638 0.16 -0.6 1,758,367 + CCNA_01638 0.16 -0.6 1,758,367 + CCNA_01638 0.16 -1.6 1,758,375 - CCNA_01638 0.17 -0.4 1,758,375 - CCNA_01638 0.17 +0.6 1,758,523 - CCNA_01638 0.27 -2.9 1,758,602 + CCNA_01638 0.33 -0.9 1,758,625 - CCNA_01638 0.35 -1.5 1,758,658 - CCNA_01638 0.37 -0.9 1,758,663 - CCNA_01638 0.38 -0.3 1,758,699 - CCNA_01638 0.40 -0.6 1,758,709 + CCNA_01638 0.41 -0.4 1,758,729 + CCNA_01638 0.42 -1.0 1,758,748 - CCNA_01638 0.44 +0.1 1,758,756 - CCNA_01638 0.44 -1.7 1,758,797 - CCNA_01638 0.47 -0.3 1,758,816 + CCNA_01638 0.49 -1.3 1,758,816 + CCNA_01638 0.49 +0.1 1,758,824 - CCNA_01638 0.49 -0.4 1,758,824 - CCNA_01638 0.49 -0.7 1,758,849 - CCNA_01638 0.51 -0.1 1,758,929 + CCNA_01638 0.57 -0.5 1,758,937 - CCNA_01638 0.57 +0.3 1,758,956 - CCNA_01638 0.59 -0.7 1,758,956 - CCNA_01638 0.59 -1.5 1,758,996 - CCNA_01638 0.61 +0.3 1,759,045 + CCNA_01638 0.65 -1.8 1,759,153 + CCNA_01638 0.73 -1.6 1,759,456 + -0.0 1,759,456 + +0.1 1,759,464 - +0.6 1,759,464 - -0.1 1,759,531 + +0.9 1,759,612 - -0.3 1,759,709 + +0.4 1,759,714 + +0.2 1,759,804 - CCNA_01639 0.12 -0.0 1,759,875 - CCNA_01639 0.18 -0.3 1,759,875 - CCNA_01639 0.18 +1.8 1,759,985 + CCNA_01639 0.26 +0.5 1,759,993 - CCNA_01639 0.27 -0.0 1,760,028 + CCNA_01639 0.29 +0.2 1,760,040 - CCNA_01639 0.30 +0.7 1,760,066 - CCNA_01639 0.32 +0.5 1,760,181 + CCNA_01639 0.41 +1.2 1,760,181 + CCNA_01639 0.41 -0.1 1,760,187 + CCNA_01639 0.42 +0.6 1,760,195 - CCNA_01639 0.42 +0.3 1,760,195 - CCNA_01639 0.42 +0.6 1,760,292 - CCNA_01639 0.50 -0.8 1,760,385 + CCNA_01639 0.57 -0.0 1,760,486 + CCNA_01639 0.64 -0.0 1,760,486 + CCNA_01639 0.64 +1.7 1,760,494 - CCNA_01639 0.65 -1.0 1,760,504 + CCNA_01639 0.66 +0.1
Or see this region's nucleotide sequence