Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01414 and CCNA_01415 are separated by 58 nucleotides CCNA_01415 and CCNA_01416 are separated by 71 nucleotides CCNA_01416 and CCNA_01417 are separated by 175 nucleotides
CCNA_01414: CCNA_01414 - 3-hydroxyisobutyryl-CoA hydrolase, at 1,531,736 to 1,532,788
_01414
CCNA_01415: CCNA_01415 - methyltransferase, at 1,532,847 to 1,533,644
_01415
CCNA_01416: CCNA_01416 - 3-hydroxyisobutyrate dehydrogenase, at 1,533,716 to 1,534,612
_01416
CCNA_01417: CCNA_01417 - 5-aminolevulinic acid synthase, at 1,534,788 to 1,536,014
_01417
Position (kb)
1533
1534
1535 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1532.770 kb on - strand at 1532.795 kb on + strand at 1532.799 kb on + strand at 1532.839 kb on + strand at 1532.847 kb on - strand at 1532.872 kb on + strand at 1532.885 kb on - strand at 1532.885 kb on - strand at 1532.971 kb on - strand, within CCNA_01415 at 1533.029 kb on + strand, within CCNA_01415 at 1533.062 kb on + strand, within CCNA_01415 at 1533.104 kb on + strand, within CCNA_01415 at 1533.125 kb on + strand, within CCNA_01415 at 1533.135 kb on + strand, within CCNA_01415 at 1533.146 kb on + strand, within CCNA_01415 at 1533.147 kb on + strand, within CCNA_01415 at 1533.152 kb on + strand, within CCNA_01415 at 1533.152 kb on + strand, within CCNA_01415 at 1533.152 kb on + strand, within CCNA_01415 at 1533.152 kb on + strand, within CCNA_01415 at 1533.152 kb on + strand, within CCNA_01415 at 1533.152 kb on + strand, within CCNA_01415 at 1533.152 kb on + strand, within CCNA_01415 at 1533.152 kb on + strand, within CCNA_01415 at 1533.152 kb on + strand, within CCNA_01415 at 1533.152 kb on + strand, within CCNA_01415 at 1533.152 kb on + strand, within CCNA_01415 at 1533.152 kb on + strand, within CCNA_01415 at 1533.153 kb on + strand, within CCNA_01415 at 1533.153 kb on + strand, within CCNA_01415 at 1533.153 kb on + strand, within CCNA_01415 at 1533.153 kb on + strand, within CCNA_01415 at 1533.153 kb on + strand, within CCNA_01415 at 1533.153 kb on + strand, within CCNA_01415 at 1533.153 kb on + strand, within CCNA_01415 at 1533.153 kb on + strand, within CCNA_01415 at 1533.153 kb on + strand, within CCNA_01415 at 1533.153 kb on + strand, within CCNA_01415 at 1533.153 kb on + strand, within CCNA_01415 at 1533.160 kb on - strand, within CCNA_01415 at 1533.160 kb on - strand, within CCNA_01415 at 1533.161 kb on - strand, within CCNA_01415 at 1533.161 kb on - strand, within CCNA_01415 at 1533.161 kb on - strand, within CCNA_01415 at 1533.194 kb on + strand, within CCNA_01415 at 1533.235 kb on + strand, within CCNA_01415 at 1533.289 kb on + strand, within CCNA_01415 at 1533.296 kb on - strand, within CCNA_01415 at 1533.332 kb on + strand, within CCNA_01415 at 1533.349 kb on + strand, within CCNA_01415 at 1533.363 kb on + strand, within CCNA_01415 at 1533.446 kb on - strand, within CCNA_01415 at 1533.454 kb on + strand, within CCNA_01415 at 1533.465 kb on - strand, within CCNA_01415 at 1533.475 kb on - strand, within CCNA_01415 at 1533.478 kb on + strand, within CCNA_01415 at 1533.478 kb on + strand, within CCNA_01415 at 1533.488 kb on + strand, within CCNA_01415 at 1533.488 kb on + strand, within CCNA_01415 at 1533.488 kb on + strand, within CCNA_01415 at 1533.488 kb on + strand, within CCNA_01415 at 1533.494 kb on + strand, within CCNA_01415 at 1533.496 kb on - strand, within CCNA_01415 at 1533.496 kb on - strand, within CCNA_01415 at 1533.501 kb on - strand, within CCNA_01415 at 1533.575 kb on + strand at 1533.748 kb on + strand at 1533.775 kb on + strand at 1533.775 kb on + strand at 1533.796 kb on - strand at 1533.827 kb on - strand, within CCNA_01416 at 1533.832 kb on - strand, within CCNA_01416 at 1533.846 kb on + strand, within CCNA_01416 at 1533.895 kb on + strand, within CCNA_01416 at 1533.912 kb on + strand, within CCNA_01416 at 1533.920 kb on - strand, within CCNA_01416 at 1533.942 kb on - strand, within CCNA_01416 at 1533.942 kb on - strand, within CCNA_01416 at 1533.944 kb on + strand, within CCNA_01416 at 1533.952 kb on - strand, within CCNA_01416 at 1533.952 kb on - strand, within CCNA_01416 at 1534.014 kb on + strand, within CCNA_01416 at 1534.019 kb on + strand, within CCNA_01416 at 1534.023 kb on + strand, within CCNA_01416 at 1534.096 kb on + strand, within CCNA_01416 at 1534.096 kb on + strand, within CCNA_01416 at 1534.104 kb on + strand, within CCNA_01416 at 1534.111 kb on - strand, within CCNA_01416 at 1534.111 kb on - strand, within CCNA_01416 at 1534.130 kb on - strand, within CCNA_01416 at 1534.158 kb on + strand, within CCNA_01416 at 1534.217 kb on + strand, within CCNA_01416 at 1534.271 kb on + strand, within CCNA_01416 at 1534.271 kb on - strand, within CCNA_01416 at 1534.279 kb on - strand, within CCNA_01416 at 1534.280 kb on - strand, within CCNA_01416 at 1534.340 kb on + strand, within CCNA_01416 at 1534.340 kb on + strand, within CCNA_01416 at 1534.340 kb on + strand, within CCNA_01416 at 1534.340 kb on + strand, within CCNA_01416 at 1534.340 kb on + strand, within CCNA_01416 at 1534.345 kb on + strand, within CCNA_01416 at 1534.345 kb on + strand, within CCNA_01416 at 1534.348 kb on - strand, within CCNA_01416 at 1534.348 kb on - strand, within CCNA_01416 at 1534.348 kb on - strand, within CCNA_01416 at 1534.348 kb on - strand, within CCNA_01416 at 1534.348 kb on - strand, within CCNA_01416 at 1534.353 kb on - strand, within CCNA_01416 at 1534.353 kb on - strand, within CCNA_01416 at 1534.363 kb on + strand, within CCNA_01416 at 1534.447 kb on - strand, within CCNA_01416 at 1534.449 kb on + strand, within CCNA_01416 at 1534.474 kb on + strand, within CCNA_01416 at 1534.531 kb on + strand at 1534.531 kb on + strand at 1534.539 kb on - strand at 1534.587 kb on + strand at 1534.587 kb on + strand at 1534.595 kb on - strand at 1534.601 kb on - strand at 1534.606 kb on + strand at 1534.699 kb on + strand at 1534.703 kb on - strand at 1534.716 kb on + strand at 1534.744 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 1,532,770 - -0.5 1,532,795 + +1.1 1,532,799 + -0.2 1,532,839 + +0.5 1,532,847 - +0.1 1,532,872 + +0.3 1,532,885 - -0.2 1,532,885 - -0.8 1,532,971 - CCNA_01415 0.16 -0.0 1,533,029 + CCNA_01415 0.23 -0.0 1,533,062 + CCNA_01415 0.27 -0.3 1,533,104 + CCNA_01415 0.32 +0.1 1,533,125 + CCNA_01415 0.35 +1.0 1,533,135 + CCNA_01415 0.36 +0.7 1,533,146 + CCNA_01415 0.37 -1.3 1,533,147 + CCNA_01415 0.38 +0.3 1,533,152 + CCNA_01415 0.38 +0.4 1,533,152 + CCNA_01415 0.38 +0.3 1,533,152 + CCNA_01415 0.38 -0.3 1,533,152 + CCNA_01415 0.38 +0.2 1,533,152 + CCNA_01415 0.38 +0.7 1,533,152 + CCNA_01415 0.38 +1.3 1,533,152 + CCNA_01415 0.38 +1.0 1,533,152 + CCNA_01415 0.38 +1.4 1,533,152 + CCNA_01415 0.38 -0.8 1,533,152 + CCNA_01415 0.38 -0.3 1,533,152 + CCNA_01415 0.38 +1.5 1,533,152 + CCNA_01415 0.38 +0.3 1,533,153 + CCNA_01415 0.38 +0.2 1,533,153 + CCNA_01415 0.38 +0.4 1,533,153 + CCNA_01415 0.38 +1.4 1,533,153 + CCNA_01415 0.38 -0.7 1,533,153 + CCNA_01415 0.38 -0.6 1,533,153 + CCNA_01415 0.38 +0.6 1,533,153 + CCNA_01415 0.38 -0.2 1,533,153 + CCNA_01415 0.38 -0.4 1,533,153 + CCNA_01415 0.38 +0.6 1,533,153 + CCNA_01415 0.38 +2.4 1,533,153 + CCNA_01415 0.38 +1.4 1,533,160 - CCNA_01415 0.39 -2.2 1,533,160 - CCNA_01415 0.39 +1.1 1,533,161 - CCNA_01415 0.39 +0.6 1,533,161 - CCNA_01415 0.39 +1.4 1,533,161 - CCNA_01415 0.39 +2.1 1,533,194 + CCNA_01415 0.43 -1.0 1,533,235 + CCNA_01415 0.49 +0.3 1,533,289 + CCNA_01415 0.55 +0.7 1,533,296 - CCNA_01415 0.56 -0.5 1,533,332 + CCNA_01415 0.61 -0.4 1,533,349 + CCNA_01415 0.63 -0.7 1,533,363 + CCNA_01415 0.65 -0.8 1,533,446 - CCNA_01415 0.75 -1.0 1,533,454 + CCNA_01415 0.76 +0.3 1,533,465 - CCNA_01415 0.77 -0.3 1,533,475 - CCNA_01415 0.79 -0.0 1,533,478 + CCNA_01415 0.79 +0.4 1,533,478 + CCNA_01415 0.79 +0.9 1,533,488 + CCNA_01415 0.80 -0.0 1,533,488 + CCNA_01415 0.80 +0.3 1,533,488 + CCNA_01415 0.80 -0.2 1,533,488 + CCNA_01415 0.80 +0.3 1,533,494 + CCNA_01415 0.81 +0.2 1,533,496 - CCNA_01415 0.81 -2.2 1,533,496 - CCNA_01415 0.81 -0.8 1,533,501 - CCNA_01415 0.82 -2.2 1,533,575 + -0.1 1,533,748 + +0.6 1,533,775 + +1.0 1,533,775 + +0.3 1,533,796 - -0.3 1,533,827 - CCNA_01416 0.12 +0.6 1,533,832 - CCNA_01416 0.13 +0.1 1,533,846 + CCNA_01416 0.14 -0.5 1,533,895 + CCNA_01416 0.20 +0.3 1,533,912 + CCNA_01416 0.22 +0.4 1,533,920 - CCNA_01416 0.23 -0.5 1,533,942 - CCNA_01416 0.25 -0.2 1,533,942 - CCNA_01416 0.25 -0.1 1,533,944 + CCNA_01416 0.25 -0.9 1,533,952 - CCNA_01416 0.26 +0.3 1,533,952 - CCNA_01416 0.26 -1.6 1,534,014 + CCNA_01416 0.33 +0.3 1,534,019 + CCNA_01416 0.34 +0.5 1,534,023 + CCNA_01416 0.34 -0.5 1,534,096 + CCNA_01416 0.42 +0.7 1,534,096 + CCNA_01416 0.42 +0.4 1,534,104 + CCNA_01416 0.43 +0.9 1,534,111 - CCNA_01416 0.44 -1.2 1,534,111 - CCNA_01416 0.44 -0.0 1,534,130 - CCNA_01416 0.46 -1.1 1,534,158 + CCNA_01416 0.49 +0.9 1,534,217 + CCNA_01416 0.56 +0.8 1,534,271 + CCNA_01416 0.62 -0.2 1,534,271 - CCNA_01416 0.62 -1.1 1,534,279 - CCNA_01416 0.63 -1.1 1,534,280 - CCNA_01416 0.63 +0.9 1,534,340 + CCNA_01416 0.70 -0.3 1,534,340 + CCNA_01416 0.70 +0.2 1,534,340 + CCNA_01416 0.70 -0.6 1,534,340 + CCNA_01416 0.70 +1.1 1,534,340 + CCNA_01416 0.70 +1.1 1,534,345 + CCNA_01416 0.70 +0.9 1,534,345 + CCNA_01416 0.70 -0.1 1,534,348 - CCNA_01416 0.70 +0.4 1,534,348 - CCNA_01416 0.70 -0.3 1,534,348 - CCNA_01416 0.70 -1.3 1,534,348 - CCNA_01416 0.70 +0.7 1,534,348 - CCNA_01416 0.70 +0.2 1,534,353 - CCNA_01416 0.71 -0.1 1,534,353 - CCNA_01416 0.71 -0.8 1,534,363 + CCNA_01416 0.72 +0.6 1,534,447 - CCNA_01416 0.81 +0.6 1,534,449 + CCNA_01416 0.82 +0.8 1,534,474 + CCNA_01416 0.85 -0.1 1,534,531 + +1.7 1,534,531 + -0.1 1,534,539 - -1.0 1,534,587 + +0.5 1,534,587 + +0.0 1,534,595 - -1.9 1,534,601 - -2.6 1,534,606 + +0.2 1,534,699 + -1.1 1,534,703 - -1.0 1,534,716 + -0.5 1,534,744 - +0.3
Or see this region's nucleotide sequence