Experiment: PYE with CHIR-090 4 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00865 and CCNA_00866 are separated by 16 nucleotides CCNA_00866 and CCNA_00867 are separated by 186 nucleotides CCNA_00867 and CCNA_03889 are separated by 254 nucleotides
CCNA_00865: CCNA_00865 - alpha-ketoglutaric semialdehyde dehydrogenase xylA, at 943,210 to 944,646
_00865
CCNA_00866: CCNA_00866 - fumarylacetoacetate hydrolase family protein, at 944,663 to 945,817
_00866
CCNA_00867: CCNA_00867 - hypothetical protein, at 946,004 to 946,492
_00867
CCNA_03889: CCNA_03889 - hypothetical protein, at 946,747 to 947,268
_03889
Position (kb) 944
945
946 Strain fitness (log2 ratio) -2
-1
0
1
2 at 943.663 kb on - strand, within CCNA_00865 at 943.694 kb on + strand, within CCNA_00865 at 943.695 kb on - strand, within CCNA_00865 at 943.702 kb on + strand, within CCNA_00865 at 943.702 kb on + strand, within CCNA_00865 at 943.749 kb on + strand, within CCNA_00865 at 943.765 kb on - strand, within CCNA_00865 at 943.782 kb on - strand, within CCNA_00865 at 943.788 kb on + strand, within CCNA_00865 at 943.810 kb on - strand, within CCNA_00865 at 943.842 kb on - strand, within CCNA_00865 at 943.866 kb on - strand, within CCNA_00865 at 943.912 kb on - strand, within CCNA_00865 at 943.921 kb on + strand, within CCNA_00865 at 943.921 kb on + strand, within CCNA_00865 at 943.938 kb on - strand, within CCNA_00865 at 943.958 kb on + strand, within CCNA_00865 at 944.047 kb on + strand, within CCNA_00865 at 944.078 kb on + strand, within CCNA_00865 at 944.134 kb on - strand, within CCNA_00865 at 944.145 kb on + strand, within CCNA_00865 at 944.214 kb on + strand, within CCNA_00865 at 944.214 kb on + strand, within CCNA_00865 at 944.214 kb on + strand, within CCNA_00865 at 944.214 kb on + strand, within CCNA_00865 at 944.214 kb on + strand, within CCNA_00865 at 944.222 kb on - strand, within CCNA_00865 at 944.222 kb on - strand, within CCNA_00865 at 944.243 kb on + strand, within CCNA_00865 at 944.278 kb on - strand, within CCNA_00865 at 944.283 kb on + strand, within CCNA_00865 at 944.301 kb on - strand, within CCNA_00865 at 944.301 kb on - strand, within CCNA_00865 at 944.307 kb on - strand, within CCNA_00865 at 944.322 kb on - strand, within CCNA_00865 at 944.362 kb on - strand, within CCNA_00865 at 944.366 kb on + strand, within CCNA_00865 at 944.366 kb on + strand, within CCNA_00865 at 944.366 kb on + strand, within CCNA_00865 at 944.366 kb on + strand, within CCNA_00865 at 944.366 kb on + strand, within CCNA_00865 at 944.366 kb on + strand, within CCNA_00865 at 944.373 kb on - strand, within CCNA_00865 at 944.374 kb on - strand, within CCNA_00865 at 944.374 kb on - strand, within CCNA_00865 at 944.374 kb on - strand, within CCNA_00865 at 944.445 kb on + strand, within CCNA_00865 at 944.445 kb on + strand, within CCNA_00865 at 944.445 kb on + strand, within CCNA_00865 at 944.479 kb on + strand, within CCNA_00865 at 944.487 kb on - strand, within CCNA_00865 at 944.519 kb on + strand at 944.544 kb on - strand at 944.558 kb on + strand at 944.566 kb on - strand at 944.582 kb on - strand at 944.642 kb on - strand at 944.642 kb on - strand at 944.644 kb on - strand at 944.693 kb on + strand at 944.693 kb on + strand at 944.701 kb on - strand at 944.701 kb on - strand at 944.701 kb on - strand at 944.721 kb on + strand at 944.730 kb on - strand at 944.757 kb on + strand at 944.761 kb on + strand at 944.815 kb on - strand, within CCNA_00866 at 944.818 kb on + strand, within CCNA_00866 at 944.834 kb on + strand, within CCNA_00866 at 944.842 kb on - strand, within CCNA_00866 at 944.872 kb on - strand, within CCNA_00866 at 944.877 kb on - strand, within CCNA_00866 at 944.901 kb on + strand, within CCNA_00866 at 944.901 kb on + strand, within CCNA_00866 at 944.909 kb on - strand, within CCNA_00866 at 944.909 kb on - strand, within CCNA_00866 at 944.909 kb on - strand, within CCNA_00866 at 944.909 kb on - strand, within CCNA_00866 at 944.909 kb on - strand, within CCNA_00866 at 944.909 kb on - strand, within CCNA_00866 at 944.914 kb on - strand, within CCNA_00866 at 944.949 kb on + strand, within CCNA_00866 at 944.952 kb on - strand, within CCNA_00866 at 945.018 kb on + strand, within CCNA_00866 at 945.036 kb on - strand, within CCNA_00866 at 945.036 kb on - strand, within CCNA_00866 at 945.086 kb on + strand, within CCNA_00866 at 945.088 kb on + strand, within CCNA_00866 at 945.095 kb on - strand, within CCNA_00866 at 945.096 kb on + strand, within CCNA_00866 at 945.129 kb on - strand, within CCNA_00866 at 945.149 kb on + strand, within CCNA_00866 at 945.162 kb on + strand, within CCNA_00866 at 945.167 kb on + strand, within CCNA_00866 at 945.167 kb on + strand, within CCNA_00866 at 945.170 kb on - strand, within CCNA_00866 at 945.172 kb on + strand, within CCNA_00866 at 945.175 kb on - strand, within CCNA_00866 at 945.175 kb on - strand, within CCNA_00866 at 945.180 kb on - strand, within CCNA_00866 at 945.206 kb on - strand, within CCNA_00866 at 945.218 kb on - strand, within CCNA_00866 at 945.269 kb on - strand, within CCNA_00866 at 945.269 kb on - strand, within CCNA_00866 at 945.274 kb on - strand, within CCNA_00866 at 945.279 kb on - strand, within CCNA_00866 at 945.279 kb on - strand, within CCNA_00866 at 945.279 kb on - strand, within CCNA_00866 at 945.279 kb on - strand, within CCNA_00866 at 945.280 kb on - strand, within CCNA_00866 at 945.318 kb on + strand, within CCNA_00866 at 945.324 kb on - strand, within CCNA_00866 at 945.324 kb on - strand, within CCNA_00866 at 945.339 kb on - strand, within CCNA_00866 at 945.340 kb on - strand, within CCNA_00866 at 945.357 kb on + strand, within CCNA_00866 at 945.487 kb on + strand, within CCNA_00866 at 945.487 kb on + strand, within CCNA_00866 at 945.487 kb on + strand, within CCNA_00866 at 945.495 kb on - strand, within CCNA_00866 at 945.502 kb on - strand, within CCNA_00866 at 945.509 kb on + strand, within CCNA_00866 at 945.517 kb on - strand, within CCNA_00866 at 945.563 kb on - strand, within CCNA_00866 at 945.565 kb on + strand, within CCNA_00866 at 945.573 kb on - strand, within CCNA_00866 at 945.580 kb on + strand, within CCNA_00866 at 945.583 kb on - strand, within CCNA_00866 at 945.585 kb on + strand, within CCNA_00866 at 945.592 kb on - strand, within CCNA_00866 at 945.621 kb on + strand, within CCNA_00866 at 945.629 kb on - strand, within CCNA_00866 at 945.629 kb on - strand, within CCNA_00866 at 945.629 kb on - strand, within CCNA_00866 at 945.629 kb on - strand, within CCNA_00866 at 945.653 kb on + strand, within CCNA_00866 at 945.663 kb on + strand, within CCNA_00866 at 945.668 kb on + strand, within CCNA_00866 at 945.681 kb on - strand, within CCNA_00866 at 945.730 kb on + strand at 945.918 kb on - strand at 945.921 kb on + strand at 945.921 kb on + strand at 945.921 kb on + strand at 945.921 kb on + strand at 945.921 kb on + strand at 945.921 kb on + strand at 945.921 kb on + strand at 945.929 kb on - strand at 945.929 kb on - strand at 945.929 kb on - strand at 945.929 kb on - strand at 945.929 kb on - strand at 945.929 kb on - strand at 945.929 kb on - strand at 945.963 kb on + strand at 945.993 kb on + strand at 945.993 kb on + strand at 945.996 kb on - strand at 946.057 kb on + strand, within CCNA_00867 at 946.057 kb on + strand, within CCNA_00867 at 946.057 kb on + strand, within CCNA_00867 at 946.115 kb on - strand, within CCNA_00867 at 946.134 kb on - strand, within CCNA_00867 at 946.143 kb on + strand, within CCNA_00867 at 946.151 kb on - strand, within CCNA_00867 at 946.172 kb on + strand, within CCNA_00867 at 946.190 kb on + strand, within CCNA_00867 at 946.190 kb on + strand, within CCNA_00867 at 946.200 kb on + strand, within CCNA_00867 at 946.232 kb on + strand, within CCNA_00867 at 946.244 kb on + strand, within CCNA_00867 at 946.245 kb on - strand, within CCNA_00867 at 946.245 kb on - strand, within CCNA_00867 at 946.245 kb on - strand, within CCNA_00867 at 946.245 kb on - strand, within CCNA_00867 at 946.312 kb on - strand, within CCNA_00867 at 946.324 kb on + strand, within CCNA_00867 at 946.324 kb on + strand, within CCNA_00867 at 946.324 kb on + strand, within CCNA_00867 at 946.324 kb on + strand, within CCNA_00867 at 946.352 kb on + strand, within CCNA_00867 at 946.376 kb on + strand, within CCNA_00867 at 946.376 kb on + strand, within CCNA_00867 at 946.376 kb on + strand, within CCNA_00867 at 946.381 kb on + strand, within CCNA_00867 at 946.384 kb on - strand, within CCNA_00867 at 946.413 kb on - strand, within CCNA_00867 at 946.415 kb on + strand, within CCNA_00867 at 946.425 kb on + strand, within CCNA_00867 at 946.430 kb on + strand, within CCNA_00867 at 946.487 kb on + strand at 946.509 kb on + strand at 946.509 kb on + strand at 946.509 kb on + strand at 946.540 kb on + strand at 946.548 kb on - strand at 946.548 kb on - strand at 946.589 kb on + strand at 946.645 kb on + strand at 946.656 kb on + strand at 946.664 kb on - strand at 946.664 kb on - strand at 946.664 kb on - strand at 946.664 kb on - strand at 946.665 kb on - strand at 946.711 kb on - strand at 946.769 kb on - strand at 946.797 kb on - strand at 946.813 kb on - strand, within CCNA_03889 at 946.815 kb on + strand, within CCNA_03889 at 946.815 kb on + strand, within CCNA_03889
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 4 ug/mL remove 943,663 - CCNA_00865 0.32 +0.2 943,694 + CCNA_00865 0.34 +1.6 943,695 - CCNA_00865 0.34 -0.2 943,702 + CCNA_00865 0.34 -1.0 943,702 + CCNA_00865 0.34 +1.5 943,749 + CCNA_00865 0.38 -0.6 943,765 - CCNA_00865 0.39 -0.5 943,782 - CCNA_00865 0.40 -0.5 943,788 + CCNA_00865 0.40 +0.5 943,810 - CCNA_00865 0.42 -0.6 943,842 - CCNA_00865 0.44 -1.1 943,866 - CCNA_00865 0.46 -0.3 943,912 - CCNA_00865 0.49 +0.5 943,921 + CCNA_00865 0.49 +0.9 943,921 + CCNA_00865 0.49 -0.3 943,938 - CCNA_00865 0.51 +0.5 943,958 + CCNA_00865 0.52 +1.0 944,047 + CCNA_00865 0.58 -0.4 944,078 + CCNA_00865 0.60 +0.6 944,134 - CCNA_00865 0.64 -0.3 944,145 + CCNA_00865 0.65 -1.0 944,214 + CCNA_00865 0.70 +0.4 944,214 + CCNA_00865 0.70 +0.3 944,214 + CCNA_00865 0.70 -0.2 944,214 + CCNA_00865 0.70 -0.5 944,214 + CCNA_00865 0.70 -1.8 944,222 - CCNA_00865 0.70 +0.4 944,222 - CCNA_00865 0.70 -0.3 944,243 + CCNA_00865 0.72 -0.2 944,278 - CCNA_00865 0.74 -1.3 944,283 + CCNA_00865 0.75 +1.6 944,301 - CCNA_00865 0.76 -2.4 944,301 - CCNA_00865 0.76 -0.9 944,307 - CCNA_00865 0.76 +0.2 944,322 - CCNA_00865 0.77 -0.9 944,362 - CCNA_00865 0.80 +0.5 944,366 + CCNA_00865 0.80 -0.8 944,366 + CCNA_00865 0.80 +0.5 944,366 + CCNA_00865 0.80 -0.5 944,366 + CCNA_00865 0.80 -0.5 944,366 + CCNA_00865 0.80 +2.6 944,366 + CCNA_00865 0.80 +0.2 944,373 - CCNA_00865 0.81 -1.1 944,374 - CCNA_00865 0.81 +0.3 944,374 - CCNA_00865 0.81 -1.1 944,374 - CCNA_00865 0.81 -1.1 944,445 + CCNA_00865 0.86 -1.3 944,445 + CCNA_00865 0.86 -1.0 944,445 + CCNA_00865 0.86 +0.2 944,479 + CCNA_00865 0.88 -0.5 944,487 - CCNA_00865 0.89 -2.2 944,519 + -1.7 944,544 - +1.7 944,558 + +0.4 944,566 - +1.5 944,582 - -1.0 944,642 - +1.9 944,642 - -0.4 944,644 - -1.7 944,693 + +0.2 944,693 + +0.9 944,701 - +0.5 944,701 - -0.7 944,701 - -0.3 944,721 + -0.5 944,730 - -0.0 944,757 + -1.0 944,761 + +0.0 944,815 - CCNA_00866 0.13 -0.1 944,818 + CCNA_00866 0.13 +0.1 944,834 + CCNA_00866 0.15 -0.2 944,842 - CCNA_00866 0.15 +0.2 944,872 - CCNA_00866 0.18 -0.1 944,877 - CCNA_00866 0.19 -0.4 944,901 + CCNA_00866 0.21 +0.3 944,901 + CCNA_00866 0.21 +0.1 944,909 - CCNA_00866 0.21 -0.1 944,909 - CCNA_00866 0.21 -0.2 944,909 - CCNA_00866 0.21 -0.8 944,909 - CCNA_00866 0.21 -0.4 944,909 - CCNA_00866 0.21 +0.6 944,909 - CCNA_00866 0.21 +0.1 944,914 - CCNA_00866 0.22 +0.3 944,949 + CCNA_00866 0.25 -2.6 944,952 - CCNA_00866 0.25 -0.4 945,018 + CCNA_00866 0.31 -0.4 945,036 - CCNA_00866 0.32 +1.2 945,036 - CCNA_00866 0.32 -0.9 945,086 + CCNA_00866 0.37 -0.1 945,088 + CCNA_00866 0.37 +0.2 945,095 - CCNA_00866 0.37 -0.3 945,096 + CCNA_00866 0.37 -1.3 945,129 - CCNA_00866 0.40 +0.8 945,149 + CCNA_00866 0.42 +0.2 945,162 + CCNA_00866 0.43 -0.2 945,167 + CCNA_00866 0.44 -0.1 945,167 + CCNA_00866 0.44 -1.1 945,170 - CCNA_00866 0.44 -0.5 945,172 + CCNA_00866 0.44 -1.2 945,175 - CCNA_00866 0.44 -1.7 945,175 - CCNA_00866 0.44 -0.5 945,180 - CCNA_00866 0.45 -0.1 945,206 - CCNA_00866 0.47 +0.1 945,218 - CCNA_00866 0.48 +1.2 945,269 - CCNA_00866 0.52 -1.3 945,269 - CCNA_00866 0.52 -1.3 945,274 - CCNA_00866 0.53 -0.6 945,279 - CCNA_00866 0.53 -1.2 945,279 - CCNA_00866 0.53 -1.1 945,279 - CCNA_00866 0.53 -0.3 945,279 - CCNA_00866 0.53 +0.5 945,280 - CCNA_00866 0.53 -0.0 945,318 + CCNA_00866 0.57 +1.0 945,324 - CCNA_00866 0.57 -0.0 945,324 - CCNA_00866 0.57 -0.1 945,339 - CCNA_00866 0.59 +0.1 945,340 - CCNA_00866 0.59 -1.4 945,357 + CCNA_00866 0.60 +1.2 945,487 + CCNA_00866 0.71 -0.3 945,487 + CCNA_00866 0.71 +0.5 945,487 + CCNA_00866 0.71 -1.6 945,495 - CCNA_00866 0.72 +0.8 945,502 - CCNA_00866 0.73 -0.5 945,509 + CCNA_00866 0.73 -0.5 945,517 - CCNA_00866 0.74 -1.5 945,563 - CCNA_00866 0.78 -0.8 945,565 + CCNA_00866 0.78 +0.1 945,573 - CCNA_00866 0.79 +0.2 945,580 + CCNA_00866 0.79 -0.5 945,583 - CCNA_00866 0.80 -0.3 945,585 + CCNA_00866 0.80 +0.3 945,592 - CCNA_00866 0.80 -0.4 945,621 + CCNA_00866 0.83 +0.7 945,629 - CCNA_00866 0.84 +0.0 945,629 - CCNA_00866 0.84 -0.3 945,629 - CCNA_00866 0.84 -0.5 945,629 - CCNA_00866 0.84 -0.3 945,653 + CCNA_00866 0.86 -2.0 945,663 + CCNA_00866 0.87 +0.1 945,668 + CCNA_00866 0.87 -1.2 945,681 - CCNA_00866 0.88 -0.2 945,730 + -2.0 945,918 - -0.5 945,921 + -1.7 945,921 + +0.3 945,921 + -0.0 945,921 + +0.4 945,921 + +0.1 945,921 + -0.6 945,921 + -0.2 945,929 - -0.3 945,929 - -0.3 945,929 - +0.8 945,929 - -2.1 945,929 - -1.2 945,929 - -0.2 945,929 - -0.4 945,963 + +1.0 945,993 + +1.0 945,993 + +0.1 945,996 - -0.4 946,057 + CCNA_00867 0.11 +0.4 946,057 + CCNA_00867 0.11 +0.6 946,057 + CCNA_00867 0.11 -0.1 946,115 - CCNA_00867 0.23 -0.1 946,134 - CCNA_00867 0.27 -0.4 946,143 + CCNA_00867 0.28 -0.1 946,151 - CCNA_00867 0.30 -1.1 946,172 + CCNA_00867 0.34 -2.6 946,190 + CCNA_00867 0.38 +0.5 946,190 + CCNA_00867 0.38 +1.4 946,200 + CCNA_00867 0.40 +0.0 946,232 + CCNA_00867 0.47 +0.3 946,244 + CCNA_00867 0.49 -0.2 946,245 - CCNA_00867 0.49 -0.3 946,245 - CCNA_00867 0.49 +0.5 946,245 - CCNA_00867 0.49 -1.6 946,245 - CCNA_00867 0.49 +0.2 946,312 - CCNA_00867 0.63 +0.7 946,324 + CCNA_00867 0.65 +0.7 946,324 + CCNA_00867 0.65 +0.8 946,324 + CCNA_00867 0.65 +0.4 946,324 + CCNA_00867 0.65 -0.0 946,352 + CCNA_00867 0.71 -0.3 946,376 + CCNA_00867 0.76 +0.3 946,376 + CCNA_00867 0.76 -0.2 946,376 + CCNA_00867 0.76 -0.2 946,381 + CCNA_00867 0.77 +2.5 946,384 - CCNA_00867 0.78 +0.6 946,413 - CCNA_00867 0.84 +1.0 946,415 + CCNA_00867 0.84 -0.4 946,425 + CCNA_00867 0.86 +0.4 946,430 + CCNA_00867 0.87 +0.2 946,487 + +0.5 946,509 + +0.0 946,509 + -0.6 946,509 + +2.3 946,540 + -0.0 946,548 - +0.2 946,548 - -0.6 946,589 + -0.6 946,645 + -0.0 946,656 + +0.0 946,664 - -0.3 946,664 - +0.2 946,664 - -0.1 946,664 - +0.2 946,665 - +1.2 946,711 - -0.1 946,769 - -0.3 946,797 - +0.7 946,813 - CCNA_03889 0.13 +0.2 946,815 + CCNA_03889 0.13 -0.2 946,815 + CCNA_03889 0.13 +0.0
Or see this region's nucleotide sequence