Experiment: 30_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS22825 and AMB_RS22830 are separated by 15 nucleotides AMB_RS22830 and AMB_RS22835 are separated by 106 nucleotides AMB_RS22835 and AMB_RS22840 are separated by 142 nucleotides AMB_RS22840 and AMB_RS22845 are separated by 72 nucleotides AMB_RS22845 and AMB_RS22850 are separated by 357 nucleotides
AMB_RS22825: AMB_RS22825 - tape measure domain-containing protein, at 4,925,199 to 4,929,788
_RS22825
AMB_RS22830: AMB_RS22830 - hypothetical protein, at 4,929,804 to 4,930,037
_RS22830
AMB_RS22835: AMB_RS22835 - hypothetical protein, at 4,930,144 to 4,930,437
_RS22835
AMB_RS22840: AMB_RS22840 - resolvase, at 4,930,580 to 4,931,245
_RS22840
AMB_RS22845: AMB_RS22845 - hypothetical protein, at 4,931,318 to 4,931,506
_RS22845
AMB_RS22850: AMB_RS22850 - hypothetical protein, at 4,931,864 to 4,932,235
_RS22850
Position (kb)
4930
4931
4932 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4929.597 kb on - strand at 4929.695 kb on + strand at 4929.695 kb on + strand at 4929.696 kb on - strand at 4929.696 kb on - strand at 4929.696 kb on - strand at 4929.789 kb on + strand at 4929.811 kb on + strand at 4929.811 kb on + strand at 4929.811 kb on + strand at 4929.934 kb on + strand, within AMB_RS22830 at 4929.934 kb on + strand, within AMB_RS22830 at 4929.935 kb on - strand, within AMB_RS22830 at 4929.935 kb on - strand, within AMB_RS22830 at 4929.935 kb on - strand, within AMB_RS22830 at 4929.953 kb on + strand, within AMB_RS22830 at 4929.953 kb on + strand, within AMB_RS22830 at 4929.954 kb on - strand, within AMB_RS22830 at 4929.954 kb on - strand, within AMB_RS22830 at 4929.986 kb on - strand, within AMB_RS22830 at 4930.076 kb on + strand at 4930.076 kb on + strand at 4930.076 kb on + strand at 4930.077 kb on - strand at 4930.077 kb on - strand at 4930.077 kb on - strand at 4930.077 kb on - strand at 4930.077 kb on - strand at 4930.100 kb on - strand at 4930.104 kb on - strand at 4930.104 kb on - strand at 4930.178 kb on + strand, within AMB_RS22835 at 4930.178 kb on + strand, within AMB_RS22835 at 4930.178 kb on + strand, within AMB_RS22835 at 4930.178 kb on + strand, within AMB_RS22835 at 4930.179 kb on - strand, within AMB_RS22835 at 4930.179 kb on - strand, within AMB_RS22835 at 4930.179 kb on - strand, within AMB_RS22835 at 4930.179 kb on - strand, within AMB_RS22835 at 4930.179 kb on - strand, within AMB_RS22835 at 4930.179 kb on - strand, within AMB_RS22835 at 4930.179 kb on - strand, within AMB_RS22835 at 4930.179 kb on - strand, within AMB_RS22835 at 4930.182 kb on + strand, within AMB_RS22835 at 4930.182 kb on + strand, within AMB_RS22835 at 4930.182 kb on + strand, within AMB_RS22835 at 4930.182 kb on + strand, within AMB_RS22835 at 4930.182 kb on + strand, within AMB_RS22835 at 4930.183 kb on - strand, within AMB_RS22835 at 4930.183 kb on - strand, within AMB_RS22835 at 4930.262 kb on + strand, within AMB_RS22835 at 4930.263 kb on - strand, within AMB_RS22835 at 4930.271 kb on + strand, within AMB_RS22835 at 4930.271 kb on + strand, within AMB_RS22835 at 4930.271 kb on + strand, within AMB_RS22835 at 4930.271 kb on + strand, within AMB_RS22835 at 4930.272 kb on - strand, within AMB_RS22835 at 4930.272 kb on - strand, within AMB_RS22835 at 4930.272 kb on - strand, within AMB_RS22835 at 4930.272 kb on - strand, within AMB_RS22835 at 4930.428 kb on - strand at 4930.560 kb on + strand at 4930.679 kb on + strand, within AMB_RS22840 at 4930.715 kb on + strand, within AMB_RS22840 at 4930.715 kb on + strand, within AMB_RS22840 at 4930.716 kb on - strand, within AMB_RS22840 at 4930.716 kb on - strand, within AMB_RS22840 at 4930.716 kb on - strand, within AMB_RS22840 at 4931.036 kb on + strand, within AMB_RS22840 at 4931.036 kb on + strand, within AMB_RS22840 at 4931.036 kb on + strand, within AMB_RS22840 at 4931.037 kb on - strand, within AMB_RS22840 at 4931.136 kb on - strand, within AMB_RS22840 at 4931.136 kb on - strand, within AMB_RS22840 at 4931.243 kb on + strand at 4931.244 kb on - strand at 4931.244 kb on - strand at 4931.597 kb on - strand at 4931.812 kb on - strand at 4931.865 kb on + strand at 4931.866 kb on - strand at 4931.866 kb on - strand at 4931.866 kb on - strand at 4931.866 kb on - strand at 4931.866 kb on - strand at 4931.866 kb on - strand at 4931.950 kb on + strand, within AMB_RS22850 at 4931.950 kb on + strand, within AMB_RS22850 at 4931.951 kb on - strand, within AMB_RS22850 at 4931.951 kb on - strand, within AMB_RS22850 at 4931.951 kb on - strand, within AMB_RS22850 at 4931.951 kb on - strand, within AMB_RS22850 at 4931.952 kb on + strand, within AMB_RS22850 at 4931.953 kb on - strand, within AMB_RS22850 at 4931.997 kb on + strand, within AMB_RS22850 at 4931.998 kb on - strand, within AMB_RS22850 at 4932.039 kb on + strand, within AMB_RS22850 at 4932.040 kb on - strand, within AMB_RS22850 at 4932.044 kb on - strand, within AMB_RS22850 at 4932.044 kb on - strand, within AMB_RS22850 at 4932.044 kb on - strand, within AMB_RS22850 at 4932.092 kb on - strand, within AMB_RS22850 at 4932.209 kb on + strand at 4932.210 kb on - strand at 4932.240 kb on + strand at 4932.240 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_3 remove 4,929,597 - -1.0 4,929,695 + -0.2 4,929,695 + +1.0 4,929,696 - -0.6 4,929,696 - -0.7 4,929,696 - -0.7 4,929,789 + -1.0 4,929,811 + -0.2 4,929,811 + -1.2 4,929,811 + -0.8 4,929,934 + AMB_RS22830 0.56 +0.2 4,929,934 + AMB_RS22830 0.56 -0.1 4,929,935 - AMB_RS22830 0.56 -0.0 4,929,935 - AMB_RS22830 0.56 -0.6 4,929,935 - AMB_RS22830 0.56 +0.3 4,929,953 + AMB_RS22830 0.64 -1.8 4,929,953 + AMB_RS22830 0.64 -0.7 4,929,954 - AMB_RS22830 0.64 -1.9 4,929,954 - AMB_RS22830 0.64 -0.4 4,929,986 - AMB_RS22830 0.78 -0.0 4,930,076 + -1.8 4,930,076 + -0.2 4,930,076 + -0.3 4,930,077 - +3.4 4,930,077 - -0.8 4,930,077 - -0.8 4,930,077 - -0.0 4,930,077 - -0.2 4,930,100 - +0.3 4,930,104 - -1.3 4,930,104 - +0.2 4,930,178 + AMB_RS22835 0.12 -0.2 4,930,178 + AMB_RS22835 0.12 -0.8 4,930,178 + AMB_RS22835 0.12 +0.1 4,930,178 + AMB_RS22835 0.12 -0.4 4,930,179 - AMB_RS22835 0.12 -1.1 4,930,179 - AMB_RS22835 0.12 +0.6 4,930,179 - AMB_RS22835 0.12 +0.1 4,930,179 - AMB_RS22835 0.12 +0.2 4,930,179 - AMB_RS22835 0.12 -0.6 4,930,179 - AMB_RS22835 0.12 -1.0 4,930,179 - AMB_RS22835 0.12 -0.2 4,930,179 - AMB_RS22835 0.12 -0.6 4,930,182 + AMB_RS22835 0.13 -1.1 4,930,182 + AMB_RS22835 0.13 -0.3 4,930,182 + AMB_RS22835 0.13 -0.3 4,930,182 + AMB_RS22835 0.13 +1.4 4,930,182 + AMB_RS22835 0.13 -0.3 4,930,183 - AMB_RS22835 0.13 -0.3 4,930,183 - AMB_RS22835 0.13 -1.4 4,930,262 + AMB_RS22835 0.40 -0.1 4,930,263 - AMB_RS22835 0.40 +0.5 4,930,271 + AMB_RS22835 0.43 -0.5 4,930,271 + AMB_RS22835 0.43 +1.1 4,930,271 + AMB_RS22835 0.43 -0.8 4,930,271 + AMB_RS22835 0.43 -0.8 4,930,272 - AMB_RS22835 0.44 -0.3 4,930,272 - AMB_RS22835 0.44 -0.9 4,930,272 - AMB_RS22835 0.44 -0.6 4,930,272 - AMB_RS22835 0.44 -0.9 4,930,428 - -1.4 4,930,560 + -1.1 4,930,679 + AMB_RS22840 0.15 -1.0 4,930,715 + AMB_RS22840 0.20 -0.5 4,930,715 + AMB_RS22840 0.20 -1.1 4,930,716 - AMB_RS22840 0.20 -2.4 4,930,716 - AMB_RS22840 0.20 -0.4 4,930,716 - AMB_RS22840 0.20 -0.3 4,931,036 + AMB_RS22840 0.68 -0.3 4,931,036 + AMB_RS22840 0.68 -0.7 4,931,036 + AMB_RS22840 0.68 -0.3 4,931,037 - AMB_RS22840 0.69 -0.4 4,931,136 - AMB_RS22840 0.83 -1.2 4,931,136 - AMB_RS22840 0.83 -0.6 4,931,243 + +1.2 4,931,244 - -0.2 4,931,244 - +0.5 4,931,597 - +0.1 4,931,812 - -2.9 4,931,865 + +0.5 4,931,866 - -0.9 4,931,866 - -1.3 4,931,866 - +0.0 4,931,866 - -0.0 4,931,866 - -0.1 4,931,866 - -0.7 4,931,950 + AMB_RS22850 0.23 -0.8 4,931,950 + AMB_RS22850 0.23 -0.1 4,931,951 - AMB_RS22850 0.23 -0.5 4,931,951 - AMB_RS22850 0.23 +0.1 4,931,951 - AMB_RS22850 0.23 +0.1 4,931,951 - AMB_RS22850 0.23 -0.3 4,931,952 + AMB_RS22850 0.24 -0.5 4,931,953 - AMB_RS22850 0.24 +0.4 4,931,997 + AMB_RS22850 0.36 -1.1 4,931,998 - AMB_RS22850 0.36 +0.4 4,932,039 + AMB_RS22850 0.47 -0.3 4,932,040 - AMB_RS22850 0.47 +0.0 4,932,044 - AMB_RS22850 0.48 +1.4 4,932,044 - AMB_RS22850 0.48 -0.8 4,932,044 - AMB_RS22850 0.48 -1.2 4,932,092 - AMB_RS22850 0.61 -0.4 4,932,209 + +0.2 4,932,210 - +1.0 4,932,240 + -0.3 4,932,240 + -0.2
Or see this region's nucleotide sequence