Experiment: 30_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS17285 and AMB_RS17290 are separated by 245 nucleotides AMB_RS17290 and AMB_RS17295 overlap by 17 nucleotides AMB_RS17295 and AMB_RS17300 are separated by 87 nucleotides
AMB_RS17285: AMB_RS17285 - RNA-binding transcriptional accessory protein, at 3,712,418 to 3,714,781
_RS17285
AMB_RS17290: AMB_RS17290 - hypothetical protein, at 3,715,027 to 3,715,467
_RS17290
AMB_RS17295: AMB_RS17295 - PilZ domain-containing protein, at 3,715,451 to 3,715,951
_RS17295
AMB_RS17300: AMB_RS17300 - hypothetical protein, at 3,716,039 to 3,716,422
_RS17300
Position (kb)
3715
3716 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3714.138 kb on + strand, within AMB_RS17285 at 3714.139 kb on - strand, within AMB_RS17285 at 3714.139 kb on - strand, within AMB_RS17285 at 3714.139 kb on - strand, within AMB_RS17285 at 3714.327 kb on + strand, within AMB_RS17285 at 3714.328 kb on - strand, within AMB_RS17285 at 3714.328 kb on - strand, within AMB_RS17285 at 3714.328 kb on - strand, within AMB_RS17285 at 3714.392 kb on + strand, within AMB_RS17285 at 3714.392 kb on + strand, within AMB_RS17285 at 3714.392 kb on + strand, within AMB_RS17285 at 3714.442 kb on - strand, within AMB_RS17285 at 3714.442 kb on - strand, within AMB_RS17285 at 3714.450 kb on + strand, within AMB_RS17285 at 3714.451 kb on - strand, within AMB_RS17285 at 3714.451 kb on - strand, within AMB_RS17285 at 3714.451 kb on - strand, within AMB_RS17285 at 3714.577 kb on - strand at 3714.820 kb on - strand at 3714.884 kb on + strand at 3715.053 kb on + strand at 3715.053 kb on + strand at 3715.053 kb on + strand at 3715.053 kb on + strand at 3715.053 kb on + strand at 3715.053 kb on + strand at 3715.053 kb on + strand at 3715.054 kb on - strand at 3715.054 kb on - strand at 3715.054 kb on - strand at 3715.372 kb on + strand, within AMB_RS17290 at 3715.373 kb on - strand, within AMB_RS17290 at 3715.833 kb on - strand, within AMB_RS17295 at 3715.965 kb on - strand at 3715.988 kb on + strand at 3715.988 kb on + strand at 3715.988 kb on + strand at 3715.988 kb on + strand at 3715.988 kb on + strand at 3715.988 kb on + strand at 3715.989 kb on - strand at 3715.989 kb on - strand at 3715.989 kb on - strand at 3715.989 kb on - strand at 3715.989 kb on - strand at 3715.989 kb on - strand at 3715.989 kb on - strand at 3715.989 kb on - strand at 3716.148 kb on + strand, within AMB_RS17300 at 3716.149 kb on - strand, within AMB_RS17300 at 3716.149 kb on - strand, within AMB_RS17300 at 3716.149 kb on - strand, within AMB_RS17300 at 3716.149 kb on - strand, within AMB_RS17300 at 3716.208 kb on + strand, within AMB_RS17300 at 3716.316 kb on + strand, within AMB_RS17300 at 3716.316 kb on + strand, within AMB_RS17300 at 3716.317 kb on - strand, within AMB_RS17300 at 3716.317 kb on - strand, within AMB_RS17300 at 3716.317 kb on - strand, within AMB_RS17300 at 3716.317 kb on - strand, within AMB_RS17300 at 3716.317 kb on - strand, within AMB_RS17300 at 3716.317 kb on - strand, within AMB_RS17300 at 3716.410 kb on + strand at 3716.446 kb on - strand at 3716.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_3 remove 3,714,138 + AMB_RS17285 0.73 -0.9 3,714,139 - AMB_RS17285 0.73 -2.0 3,714,139 - AMB_RS17285 0.73 -0.3 3,714,139 - AMB_RS17285 0.73 -1.1 3,714,327 + AMB_RS17285 0.81 -0.1 3,714,328 - AMB_RS17285 0.81 -0.3 3,714,328 - AMB_RS17285 0.81 -0.6 3,714,328 - AMB_RS17285 0.81 +0.5 3,714,392 + AMB_RS17285 0.84 -0.4 3,714,392 + AMB_RS17285 0.84 -0.3 3,714,392 + AMB_RS17285 0.84 -0.4 3,714,442 - AMB_RS17285 0.86 +2.4 3,714,442 - AMB_RS17285 0.86 +0.1 3,714,450 + AMB_RS17285 0.86 -1.6 3,714,451 - AMB_RS17285 0.86 -0.2 3,714,451 - AMB_RS17285 0.86 +1.5 3,714,451 - AMB_RS17285 0.86 -0.6 3,714,577 - -0.1 3,714,820 - -0.0 3,714,884 + +0.2 3,715,053 + -0.7 3,715,053 + +0.2 3,715,053 + -0.9 3,715,053 + -0.2 3,715,053 + +0.1 3,715,053 + -0.3 3,715,053 + -0.3 3,715,054 - +1.1 3,715,054 - -0.7 3,715,054 - -1.4 3,715,372 + AMB_RS17290 0.78 -0.0 3,715,373 - AMB_RS17290 0.78 +0.1 3,715,833 - AMB_RS17295 0.76 -0.2 3,715,965 - -0.5 3,715,988 + -0.2 3,715,988 + +0.4 3,715,988 + -2.8 3,715,988 + -2.2 3,715,988 + -0.4 3,715,988 + -0.2 3,715,989 - +0.9 3,715,989 - -0.1 3,715,989 - -0.4 3,715,989 - -0.1 3,715,989 - -1.3 3,715,989 - -0.1 3,715,989 - -1.2 3,715,989 - -0.3 3,716,148 + AMB_RS17300 0.28 +0.4 3,716,149 - AMB_RS17300 0.29 +0.2 3,716,149 - AMB_RS17300 0.29 -0.4 3,716,149 - AMB_RS17300 0.29 +0.1 3,716,149 - AMB_RS17300 0.29 +0.1 3,716,208 + AMB_RS17300 0.44 -0.3 3,716,316 + AMB_RS17300 0.72 -0.4 3,716,316 + AMB_RS17300 0.72 -0.2 3,716,317 - AMB_RS17300 0.72 -0.3 3,716,317 - AMB_RS17300 0.72 +0.2 3,716,317 - AMB_RS17300 0.72 +0.8 3,716,317 - AMB_RS17300 0.72 -1.1 3,716,317 - AMB_RS17300 0.72 -0.2 3,716,317 - AMB_RS17300 0.72 -0.2 3,716,410 + +0.7 3,716,446 - -0.4 3,716,457 - +0.1
Or see this region's nucleotide sequence