Experiment: 30_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07605 and AMB_RS07610 are separated by 4 nucleotides AMB_RS07610 and AMB_RS07615 are separated by 88 nucleotides AMB_RS07615 and AMB_RS07620 are separated by 7 nucleotides AMB_RS07620 and AMB_RS07625 overlap by 4 nucleotides AMB_RS07625 and AMB_RS07630 are separated by 20 nucleotides AMB_RS07630 and AMB_RS23305 overlap by 4 nucleotides
AMB_RS07605: AMB_RS07605 - hypothetical protein, at 1,642,491 to 1,642,673
_RS07605
AMB_RS07610: AMB_RS07610 - hypothetical protein, at 1,642,678 to 1,642,893
_RS07610
AMB_RS07615: AMB_RS07615 - hypothetical protein, at 1,642,982 to 1,643,251
_RS07615
AMB_RS07620: AMB_RS07620 - hypothetical protein, at 1,643,259 to 1,643,597
_RS07620
AMB_RS07625: AMB_RS07625 - hypothetical protein, at 1,643,594 to 1,644,244
_RS07625
AMB_RS07630: AMB_RS07630 - hypothetical protein, at 1,644,265 to 1,645,008
_RS07630
AMB_RS23305: AMB_RS23305 - hypothetical protein, at 1,645,005 to 1,645,634
_RS23305
Position (kb)
1643
1644
1645 Strain fitness (log2 ratio)
-2
-1
0
1 at 1642.603 kb on + strand, within AMB_RS07605 at 1642.603 kb on + strand, within AMB_RS07605 at 1643.058 kb on - strand, within AMB_RS07615 at 1643.259 kb on - strand at 1643.259 kb on - strand at 1643.259 kb on - strand at 1643.259 kb on - strand at 1643.587 kb on + strand at 1643.588 kb on - strand at 1644.135 kb on - strand, within AMB_RS07625 at 1644.135 kb on - strand, within AMB_RS07625 at 1644.135 kb on - strand, within AMB_RS07625 at 1644.611 kb on + strand, within AMB_RS07630 at 1644.612 kb on - strand, within AMB_RS07630 at 1644.642 kb on - strand, within AMB_RS07630 at 1644.777 kb on + strand, within AMB_RS07630
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_3 remove 1,642,603 + AMB_RS07605 0.61 -0.4 1,642,603 + AMB_RS07605 0.61 +0.2 1,643,058 - AMB_RS07615 0.28 -2.0 1,643,259 - -0.2 1,643,259 - -0.1 1,643,259 - -0.9 1,643,259 - +0.4 1,643,587 + -0.6 1,643,588 - +1.3 1,644,135 - AMB_RS07625 0.83 -0.3 1,644,135 - AMB_RS07625 0.83 -1.4 1,644,135 - AMB_RS07625 0.83 -0.7 1,644,611 + AMB_RS07630 0.47 +0.3 1,644,612 - AMB_RS07630 0.47 +0.4 1,644,642 - AMB_RS07630 0.51 -1.0 1,644,777 + AMB_RS07630 0.69 +0.0
Or see this region's nucleotide sequence