Experiment: 30_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS06290 and AMB_RS06295 are separated by 397 nucleotides AMB_RS06295 and AMB_RS06300 overlap by 4 nucleotides
AMB_RS06290: AMB_RS06290 - hypothetical protein, at 1,318,772 to 1,319,632
_RS06290
AMB_RS06295: AMB_RS06295 - LysR family transcriptional regulator, at 1,320,030 to 1,320,440
_RS06295
AMB_RS06300: AMB_RS06300 - phenylacetyl CoA, at 1,320,437 to 1,323,205
_RS06300
Position (kb)
1320
1321 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1319.366 kb on + strand, within AMB_RS06290 at 1319.396 kb on + strand, within AMB_RS06290 at 1319.396 kb on + strand, within AMB_RS06290 at 1319.396 kb on + strand, within AMB_RS06290 at 1319.397 kb on - strand, within AMB_RS06290 at 1319.397 kb on - strand, within AMB_RS06290 at 1319.438 kb on + strand, within AMB_RS06290 at 1319.439 kb on - strand, within AMB_RS06290 at 1319.439 kb on - strand, within AMB_RS06290 at 1319.713 kb on - strand at 1319.769 kb on + strand at 1319.888 kb on + strand at 1319.889 kb on - strand at 1319.924 kb on + strand at 1319.924 kb on + strand at 1319.947 kb on + strand at 1319.948 kb on - strand at 1319.963 kb on + strand at 1320.073 kb on - strand, within AMB_RS06295 at 1320.126 kb on + strand, within AMB_RS06295 at 1320.126 kb on + strand, within AMB_RS06295 at 1320.127 kb on - strand, within AMB_RS06295 at 1320.127 kb on - strand, within AMB_RS06295 at 1320.147 kb on + strand, within AMB_RS06295 at 1320.147 kb on + strand, within AMB_RS06295 at 1320.148 kb on - strand, within AMB_RS06295 at 1320.148 kb on - strand, within AMB_RS06295 at 1320.491 kb on + strand at 1320.491 kb on + strand at 1320.491 kb on + strand at 1320.491 kb on + strand at 1320.491 kb on + strand at 1320.492 kb on - strand at 1320.492 kb on - strand at 1320.492 kb on - strand at 1320.497 kb on + strand at 1320.498 kb on - strand at 1320.498 kb on - strand at 1320.645 kb on - strand at 1320.854 kb on + strand, within AMB_RS06300 at 1320.855 kb on - strand, within AMB_RS06300 at 1320.855 kb on - strand, within AMB_RS06300 at 1320.855 kb on - strand, within AMB_RS06300 at 1320.855 kb on - strand, within AMB_RS06300 at 1320.855 kb on - strand, within AMB_RS06300 at 1320.935 kb on + strand, within AMB_RS06300 at 1320.936 kb on - strand, within AMB_RS06300 at 1320.954 kb on - strand, within AMB_RS06300 at 1320.977 kb on + strand, within AMB_RS06300 at 1320.977 kb on + strand, within AMB_RS06300 at 1320.978 kb on - strand, within AMB_RS06300 at 1320.978 kb on - strand, within AMB_RS06300 at 1321.010 kb on + strand, within AMB_RS06300 at 1321.010 kb on + strand, within AMB_RS06300 at 1321.010 kb on + strand, within AMB_RS06300 at 1321.010 kb on + strand, within AMB_RS06300 at 1321.010 kb on + strand, within AMB_RS06300 at 1321.011 kb on - strand, within AMB_RS06300 at 1321.011 kb on - strand, within AMB_RS06300 at 1321.011 kb on - strand, within AMB_RS06300 at 1321.011 kb on - strand, within AMB_RS06300 at 1321.011 kb on - strand, within AMB_RS06300 at 1321.034 kb on - strand, within AMB_RS06300 at 1321.065 kb on - strand, within AMB_RS06300 at 1321.065 kb on - strand, within AMB_RS06300 at 1321.274 kb on + strand, within AMB_RS06300 at 1321.274 kb on + strand, within AMB_RS06300 at 1321.274 kb on + strand, within AMB_RS06300 at 1321.299 kb on - strand, within AMB_RS06300
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_3 remove 1,319,366 + AMB_RS06290 0.69 +0.0 1,319,396 + AMB_RS06290 0.72 -0.8 1,319,396 + AMB_RS06290 0.72 -0.8 1,319,396 + AMB_RS06290 0.72 -2.0 1,319,397 - AMB_RS06290 0.73 +0.7 1,319,397 - AMB_RS06290 0.73 -0.4 1,319,438 + AMB_RS06290 0.77 -0.4 1,319,439 - AMB_RS06290 0.77 +0.1 1,319,439 - AMB_RS06290 0.77 -1.1 1,319,713 - -0.5 1,319,769 + -0.6 1,319,888 + +0.1 1,319,889 - -0.4 1,319,924 + -0.1 1,319,924 + -0.6 1,319,947 + -1.2 1,319,948 - -2.6 1,319,963 + +0.9 1,320,073 - AMB_RS06295 0.10 -2.8 1,320,126 + AMB_RS06295 0.23 -0.5 1,320,126 + AMB_RS06295 0.23 -0.4 1,320,127 - AMB_RS06295 0.24 -1.1 1,320,127 - AMB_RS06295 0.24 -1.0 1,320,147 + AMB_RS06295 0.28 -2.6 1,320,147 + AMB_RS06295 0.28 -0.4 1,320,148 - AMB_RS06295 0.29 +0.7 1,320,148 - AMB_RS06295 0.29 -0.6 1,320,491 + +0.2 1,320,491 + -0.4 1,320,491 + -3.2 1,320,491 + -1.1 1,320,491 + -2.3 1,320,492 - -0.1 1,320,492 - -0.7 1,320,492 - -0.1 1,320,497 + -2.4 1,320,498 - -2.4 1,320,498 - -0.5 1,320,645 - +0.4 1,320,854 + AMB_RS06300 0.15 -0.4 1,320,855 - AMB_RS06300 0.15 -0.4 1,320,855 - AMB_RS06300 0.15 -0.4 1,320,855 - AMB_RS06300 0.15 -0.1 1,320,855 - AMB_RS06300 0.15 -0.5 1,320,855 - AMB_RS06300 0.15 -2.4 1,320,935 + AMB_RS06300 0.18 -0.7 1,320,936 - AMB_RS06300 0.18 -0.7 1,320,954 - AMB_RS06300 0.19 -0.7 1,320,977 + AMB_RS06300 0.20 -0.6 1,320,977 + AMB_RS06300 0.20 -0.8 1,320,978 - AMB_RS06300 0.20 -0.4 1,320,978 - AMB_RS06300 0.20 +0.9 1,321,010 + AMB_RS06300 0.21 -0.9 1,321,010 + AMB_RS06300 0.21 -1.9 1,321,010 + AMB_RS06300 0.21 -0.4 1,321,010 + AMB_RS06300 0.21 +0.4 1,321,010 + AMB_RS06300 0.21 +0.2 1,321,011 - AMB_RS06300 0.21 -0.6 1,321,011 - AMB_RS06300 0.21 -1.6 1,321,011 - AMB_RS06300 0.21 -1.0 1,321,011 - AMB_RS06300 0.21 -0.4 1,321,011 - AMB_RS06300 0.21 +2.0 1,321,034 - AMB_RS06300 0.22 -0.4 1,321,065 - AMB_RS06300 0.23 +0.2 1,321,065 - AMB_RS06300 0.23 -1.0 1,321,274 + AMB_RS06300 0.30 +0.7 1,321,274 + AMB_RS06300 0.30 +0.8 1,321,274 + AMB_RS06300 0.30 +0.2 1,321,299 - AMB_RS06300 0.31 +0.2
Or see this region's nucleotide sequence