Experiment: 30_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04000 and AMB_RS04005 are separated by 346 nucleotides AMB_RS04005 and AMB_RS04010 are separated by 29 nucleotides AMB_RS04010 and AMB_RS04015 are separated by 244 nucleotides
AMB_RS04000: AMB_RS04000 - XRE family transcriptional regulator, at 838,662 to 839,054
_RS04000
AMB_RS04005: AMB_RS04005 - hypothetical protein, at 839,401 to 839,871
_RS04005
AMB_RS04010: AMB_RS04010 - putative heme iron utilization protein, at 839,901 to 840,788
_RS04010
AMB_RS04015: AMB_RS04015 - phosphoenolpyruvate carboxykinase (GTP), at 841,033 to 842,886
_RS04015
Position (kb) 839
840
841 Strain fitness (log2 ratio) -1
0
1 at 838.948 kb on - strand, within AMB_RS04000 at 839.122 kb on - strand at 839.122 kb on - strand at 839.137 kb on + strand at 839.138 kb on - strand at 839.176 kb on + strand at 839.198 kb on + strand at 839.239 kb on - strand at 839.327 kb on + strand at 839.327 kb on + strand at 839.328 kb on - strand at 839.328 kb on - strand at 839.331 kb on + strand at 839.332 kb on - strand at 839.374 kb on + strand at 839.388 kb on + strand at 839.388 kb on + strand at 839.389 kb on - strand at 839.403 kb on - strand at 839.462 kb on + strand, within AMB_RS04005 at 839.462 kb on + strand, within AMB_RS04005 at 839.462 kb on + strand, within AMB_RS04005 at 839.462 kb on + strand, within AMB_RS04005 at 839.463 kb on - strand, within AMB_RS04005 at 839.463 kb on - strand, within AMB_RS04005 at 839.519 kb on + strand, within AMB_RS04005 at 839.535 kb on - strand, within AMB_RS04005 at 839.535 kb on - strand, within AMB_RS04005 at 839.905 kb on + strand at 839.906 kb on - strand at 839.906 kb on - strand at 839.908 kb on + strand at 839.909 kb on - strand at 839.909 kb on - strand at 839.909 kb on - strand at 839.981 kb on - strand at 840.177 kb on + strand, within AMB_RS04010 at 840.177 kb on + strand, within AMB_RS04010 at 840.178 kb on - strand, within AMB_RS04010 at 840.178 kb on - strand, within AMB_RS04010 at 840.178 kb on - strand, within AMB_RS04010 at 840.178 kb on - strand, within AMB_RS04010 at 840.420 kb on + strand, within AMB_RS04010 at 840.420 kb on + strand, within AMB_RS04010 at 840.421 kb on - strand, within AMB_RS04010 at 840.926 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_3 remove 838,948 - AMB_RS04000 0.73 -0.2 839,122 - +1.2 839,122 - +0.4 839,137 + -0.4 839,138 - -0.3 839,176 + -1.3 839,198 + -0.6 839,239 - -0.6 839,327 + +0.1 839,327 + -1.0 839,328 - -0.5 839,328 - -0.2 839,331 + +1.0 839,332 - -0.6 839,374 + -0.2 839,388 + -0.9 839,388 + -0.1 839,389 - +0.3 839,403 - -1.2 839,462 + AMB_RS04005 0.13 -0.0 839,462 + AMB_RS04005 0.13 -1.5 839,462 + AMB_RS04005 0.13 +0.0 839,462 + AMB_RS04005 0.13 +0.1 839,463 - AMB_RS04005 0.13 +1.7 839,463 - AMB_RS04005 0.13 +0.2 839,519 + AMB_RS04005 0.25 -0.9 839,535 - AMB_RS04005 0.28 +0.7 839,535 - AMB_RS04005 0.28 +0.6 839,905 + -0.5 839,906 - +0.1 839,906 - -0.6 839,908 + -1.6 839,909 - -0.0 839,909 - -0.8 839,909 - -0.0 839,981 - -0.5 840,177 + AMB_RS04010 0.31 -0.5 840,177 + AMB_RS04010 0.31 +0.4 840,178 - AMB_RS04010 0.31 -0.4 840,178 - AMB_RS04010 0.31 -0.2 840,178 - AMB_RS04010 0.31 -0.0 840,178 - AMB_RS04010 0.31 +0.7 840,420 + AMB_RS04010 0.58 +0.9 840,420 + AMB_RS04010 0.58 +0.0 840,421 - AMB_RS04010 0.59 -0.6 840,926 + -1.6
Or see this region's nucleotide sequence