Experiment: 30_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03895 and AMB_RS03900 are separated by 128 nucleotides AMB_RS03900 and AMB_RS03905 are separated by 102 nucleotides AMB_RS03905 and AMB_RS03910 overlap by 4 nucleotides
AMB_RS03895: AMB_RS03895 - response regulator, at 817,405 to 818,133
_RS03895
AMB_RS03900: AMB_RS03900 - hypothetical protein, at 818,262 to 818,972
_RS03900
AMB_RS03905: AMB_RS03905 - ribonuclease H, at 819,075 to 819,539
_RS03905
AMB_RS03910: AMB_RS03910 - homoserine kinase, at 819,536 to 820,516
_RS03910
Position (kb)
819
820 Strain fitness (log2 ratio)
-2
-1
0
1 at 818.131 kb on + strand at 818.218 kb on - strand at 818.218 kb on - strand at 818.278 kb on - strand at 818.496 kb on + strand, within AMB_RS03900 at 818.496 kb on + strand, within AMB_RS03900 at 818.496 kb on + strand, within AMB_RS03900 at 818.496 kb on + strand, within AMB_RS03900 at 818.497 kb on - strand, within AMB_RS03900 at 818.497 kb on - strand, within AMB_RS03900 at 818.673 kb on + strand, within AMB_RS03900 at 818.673 kb on + strand, within AMB_RS03900 at 818.674 kb on - strand, within AMB_RS03900 at 818.674 kb on - strand, within AMB_RS03900 at 818.674 kb on - strand, within AMB_RS03900 at 818.895 kb on + strand, within AMB_RS03900 at 818.895 kb on + strand, within AMB_RS03900 at 819.076 kb on + strand at 819.076 kb on + strand at 819.076 kb on + strand at 819.076 kb on + strand at 819.077 kb on - strand at 819.077 kb on - strand at 819.077 kb on - strand at 819.077 kb on - strand at 819.077 kb on - strand at 819.077 kb on - strand at 819.304 kb on + strand, within AMB_RS03905 at 819.304 kb on + strand, within AMB_RS03905 at 819.305 kb on - strand, within AMB_RS03905 at 819.305 kb on - strand, within AMB_RS03905 at 819.305 kb on - strand, within AMB_RS03905 at 819.319 kb on + strand, within AMB_RS03905 at 819.319 kb on + strand, within AMB_RS03905 at 819.499 kb on + strand at 819.499 kb on + strand at 819.499 kb on + strand at 819.500 kb on - strand at 819.500 kb on - strand at 819.500 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_3 remove 818,131 + -0.7 818,218 - -0.5 818,218 - -0.4 818,278 - +0.1 818,496 + AMB_RS03900 0.33 +1.4 818,496 + AMB_RS03900 0.33 +0.4 818,496 + AMB_RS03900 0.33 +0.2 818,496 + AMB_RS03900 0.33 -0.2 818,497 - AMB_RS03900 0.33 -0.8 818,497 - AMB_RS03900 0.33 -0.4 818,673 + AMB_RS03900 0.58 -0.4 818,673 + AMB_RS03900 0.58 +0.0 818,674 - AMB_RS03900 0.58 -0.8 818,674 - AMB_RS03900 0.58 -2.2 818,674 - AMB_RS03900 0.58 +0.1 818,895 + AMB_RS03900 0.89 -0.9 818,895 + AMB_RS03900 0.89 -0.4 819,076 + -0.0 819,076 + -0.8 819,076 + -0.4 819,076 + +0.4 819,077 - -0.0 819,077 - -0.2 819,077 - -0.9 819,077 - +0.8 819,077 - -0.9 819,077 - -0.5 819,304 + AMB_RS03905 0.49 +0.8 819,304 + AMB_RS03905 0.49 -0.8 819,305 - AMB_RS03905 0.49 +0.4 819,305 - AMB_RS03905 0.49 +0.0 819,305 - AMB_RS03905 0.49 +0.6 819,319 + AMB_RS03905 0.52 +0.1 819,319 + AMB_RS03905 0.52 -0.9 819,499 + -0.3 819,499 + -0.6 819,499 + +0.1 819,500 - +0.5 819,500 - -0.2 819,500 - -0.9
Or see this region's nucleotide sequence