Experiment: 30_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS02545 and AMB_RS02550 are separated by 94 nucleotides AMB_RS02550 and AMB_RS02555 are separated by 7 nucleotides AMB_RS02555 and AMB_RS02560 overlap by 4 nucleotides
AMB_RS02545: AMB_RS02545 - hypothetical protein, at 526,279 to 527,778
_RS02545
AMB_RS02550: AMB_RS02550 - DNA polymerase III, at 527,873 to 529,633
_RS02550
AMB_RS02555: AMB_RS02555 - cobyrinic acid a,c-diamide synthase, at 529,641 to 530,438
_RS02555
AMB_RS02560: AMB_RS02560 - GTP-binding protein, at 530,435 to 531,436
_RS02560
Position (kb) 527
528
529
530 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 527.459 kb on + strand, within AMB_RS02545 at 527.459 kb on + strand, within AMB_RS02545 at 527.460 kb on - strand, within AMB_RS02545 at 527.460 kb on - strand, within AMB_RS02545 at 527.460 kb on - strand, within AMB_RS02545 at 527.460 kb on - strand, within AMB_RS02545 at 527.460 kb on - strand, within AMB_RS02545 at 527.468 kb on + strand, within AMB_RS02545 at 527.468 kb on + strand, within AMB_RS02545 at 527.468 kb on + strand, within AMB_RS02545 at 527.468 kb on + strand, within AMB_RS02545 at 527.570 kb on + strand, within AMB_RS02545 at 527.744 kb on + strand at 527.744 kb on + strand at 527.744 kb on + strand at 527.745 kb on - strand at 527.745 kb on - strand at 527.747 kb on + strand at 527.834 kb on + strand at 527.877 kb on - strand at 527.877 kb on - strand at 527.877 kb on - strand at 527.877 kb on - strand at 527.877 kb on - strand at 528.187 kb on - strand, within AMB_RS02550 at 528.187 kb on - strand, within AMB_RS02550 at 528.954 kb on - strand, within AMB_RS02550 at 528.954 kb on - strand, within AMB_RS02550 at 529.246 kb on + strand, within AMB_RS02550 at 529.642 kb on + strand at 529.828 kb on + strand, within AMB_RS02555 at 529.828 kb on + strand, within AMB_RS02555 at 529.828 kb on + strand, within AMB_RS02555 at 529.829 kb on - strand, within AMB_RS02555 at 529.829 kb on - strand, within AMB_RS02555 at 529.829 kb on - strand, within AMB_RS02555 at 529.924 kb on + strand, within AMB_RS02555 at 529.924 kb on + strand, within AMB_RS02555 at 529.924 kb on + strand, within AMB_RS02555 at 529.925 kb on - strand, within AMB_RS02555 at 529.925 kb on - strand, within AMB_RS02555 at 530.044 kb on + strand, within AMB_RS02555 at 530.044 kb on + strand, within AMB_RS02555 at 530.045 kb on - strand, within AMB_RS02555
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_3 remove 527,459 + AMB_RS02545 0.79 -0.1 527,459 + AMB_RS02545 0.79 -0.2 527,460 - AMB_RS02545 0.79 +0.1 527,460 - AMB_RS02545 0.79 -2.1 527,460 - AMB_RS02545 0.79 -0.0 527,460 - AMB_RS02545 0.79 -0.2 527,460 - AMB_RS02545 0.79 -0.1 527,468 + AMB_RS02545 0.79 +0.2 527,468 + AMB_RS02545 0.79 -1.3 527,468 + AMB_RS02545 0.79 +0.4 527,468 + AMB_RS02545 0.79 +0.0 527,570 + AMB_RS02545 0.86 -0.3 527,744 + -1.8 527,744 + +0.7 527,744 + +0.3 527,745 - -0.7 527,745 - -1.5 527,747 + -0.6 527,834 + +0.5 527,877 - -0.1 527,877 - +0.0 527,877 - -0.2 527,877 - -0.5 527,877 - -1.9 528,187 - AMB_RS02550 0.18 +0.1 528,187 - AMB_RS02550 0.18 -0.1 528,954 - AMB_RS02550 0.61 +4.5 528,954 - AMB_RS02550 0.61 +0.7 529,246 + AMB_RS02550 0.78 +0.8 529,642 + -0.7 529,828 + AMB_RS02555 0.23 -1.3 529,828 + AMB_RS02555 0.23 +1.4 529,828 + AMB_RS02555 0.23 -0.2 529,829 - AMB_RS02555 0.24 +0.1 529,829 - AMB_RS02555 0.24 +1.0 529,829 - AMB_RS02555 0.24 -0.1 529,924 + AMB_RS02555 0.35 +0.2 529,924 + AMB_RS02555 0.35 -0.1 529,924 + AMB_RS02555 0.35 -1.2 529,925 - AMB_RS02555 0.36 -1.4 529,925 - AMB_RS02555 0.36 +0.4 530,044 + AMB_RS02555 0.51 -2.9 530,044 + AMB_RS02555 0.51 +0.1 530,045 - AMB_RS02555 0.51 +0.1
Or see this region's nucleotide sequence