Experiment: 30_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23890 and AMB_RS02500 are separated by 57 nucleotides AMB_RS02500 and AMB_RS02505 overlap by 4 nucleotides AMB_RS02505 and AMB_RS02510 are separated by 512 nucleotides
AMB_RS23890: AMB_RS23890 - hypothetical protein, at 517,361 to 518,557
_RS23890
AMB_RS02500: AMB_RS02500 - hypothetical protein, at 518,615 to 518,851
_RS02500
AMB_RS02505: AMB_RS02505 - hypothetical protein, at 518,848 to 519,033
_RS02505
AMB_RS02510: AMB_RS02510 - DUF4102 domain-containing protein, at 519,546 to 520,784
_RS02510
Position (kb) 518
519
520 Strain fitness (log2 ratio) -2
-1
0
1
2 at 517.883 kb on - strand, within AMB_RS23890 at 517.990 kb on + strand, within AMB_RS23890 at 518.081 kb on - strand, within AMB_RS23890 at 518.239 kb on - strand, within AMB_RS23890 at 518.384 kb on + strand, within AMB_RS23890 at 518.385 kb on - strand, within AMB_RS23890 at 518.585 kb on + strand at 518.616 kb on + strand at 518.655 kb on + strand, within AMB_RS02500 at 518.655 kb on + strand, within AMB_RS02500 at 518.655 kb on + strand, within AMB_RS02500 at 518.700 kb on - strand, within AMB_RS02500 at 518.700 kb on - strand, within AMB_RS02500 at 518.700 kb on - strand, within AMB_RS02500 at 518.700 kb on - strand, within AMB_RS02500 at 518.892 kb on + strand, within AMB_RS02505 at 518.892 kb on + strand, within AMB_RS02505 at 518.892 kb on + strand, within AMB_RS02505 at 518.893 kb on - strand, within AMB_RS02505 at 518.895 kb on - strand, within AMB_RS02505 at 518.949 kb on + strand, within AMB_RS02505 at 518.950 kb on - strand, within AMB_RS02505 at 518.957 kb on + strand, within AMB_RS02505 at 519.008 kb on - strand, within AMB_RS02505 at 519.077 kb on + strand at 519.078 kb on - strand at 519.078 kb on - strand at 519.078 kb on - strand at 519.078 kb on - strand at 519.085 kb on + strand at 519.085 kb on + strand at 519.124 kb on - strand at 519.286 kb on + strand at 519.287 kb on - strand at 519.287 kb on - strand at 519.468 kb on - strand at 519.499 kb on + strand at 519.499 kb on + strand at 519.499 kb on + strand at 519.500 kb on - strand at 519.500 kb on - strand at 519.536 kb on - strand at 519.536 kb on - strand at 519.585 kb on + strand at 519.617 kb on - strand at 519.617 kb on - strand at 519.658 kb on + strand at 519.658 kb on + strand at 519.658 kb on + strand at 519.659 kb on - strand at 519.659 kb on - strand at 519.674 kb on - strand, within AMB_RS02510 at 519.674 kb on - strand, within AMB_RS02510 at 519.674 kb on - strand, within AMB_RS02510 at 519.674 kb on - strand, within AMB_RS02510 at 519.674 kb on - strand, within AMB_RS02510 at 519.674 kb on - strand, within AMB_RS02510 at 519.811 kb on + strand, within AMB_RS02510 at 519.837 kb on + strand, within AMB_RS02510 at 519.945 kb on + strand, within AMB_RS02510
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_3 remove 517,883 - AMB_RS23890 0.44 -0.1 517,990 + AMB_RS23890 0.53 +0.7 518,081 - AMB_RS23890 0.60 +0.0 518,239 - AMB_RS23890 0.73 +0.4 518,384 + AMB_RS23890 0.85 +0.2 518,385 - AMB_RS23890 0.86 -1.1 518,585 + -1.0 518,616 + +0.5 518,655 + AMB_RS02500 0.17 +0.1 518,655 + AMB_RS02500 0.17 +1.2 518,655 + AMB_RS02500 0.17 +0.4 518,700 - AMB_RS02500 0.36 -1.6 518,700 - AMB_RS02500 0.36 -0.2 518,700 - AMB_RS02500 0.36 +0.0 518,700 - AMB_RS02500 0.36 +0.4 518,892 + AMB_RS02505 0.24 -0.8 518,892 + AMB_RS02505 0.24 +0.2 518,892 + AMB_RS02505 0.24 +0.0 518,893 - AMB_RS02505 0.24 +0.3 518,895 - AMB_RS02505 0.25 -1.1 518,949 + AMB_RS02505 0.54 -0.6 518,950 - AMB_RS02505 0.55 -0.7 518,957 + AMB_RS02505 0.59 +2.4 519,008 - AMB_RS02505 0.86 -0.7 519,077 + +1.0 519,078 - -1.0 519,078 - -1.3 519,078 - +0.8 519,078 - -2.6 519,085 + +0.4 519,085 + -0.0 519,124 - +0.6 519,286 + -0.3 519,287 - +0.0 519,287 - -0.2 519,468 - -1.2 519,499 + -0.3 519,499 + -0.2 519,499 + -0.8 519,500 - -1.2 519,500 - +0.1 519,536 - -0.5 519,536 - -0.4 519,585 + +0.2 519,617 - -0.1 519,617 - -2.1 519,658 + -0.2 519,658 + -0.5 519,658 + +0.2 519,659 - -0.5 519,659 - +0.9 519,674 - AMB_RS02510 0.10 -0.4 519,674 - AMB_RS02510 0.10 -0.8 519,674 - AMB_RS02510 0.10 +0.3 519,674 - AMB_RS02510 0.10 -0.0 519,674 - AMB_RS02510 0.10 -0.2 519,674 - AMB_RS02510 0.10 -0.1 519,811 + AMB_RS02510 0.21 +0.4 519,837 + AMB_RS02510 0.23 -1.8 519,945 + AMB_RS02510 0.32 -2.3
Or see this region's nucleotide sequence