Experiment: 30_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00075 and AMB_RS00080 are separated by 386 nucleotides AMB_RS00080 and AMB_RS00085 are separated by 312 nucleotides
AMB_RS00075: AMB_RS00075 - P-II family nitrogen regulator, at 11,477 to 11,815
_RS00075
AMB_RS00080: AMB_RS00080 - ammonium transporter, at 12,202 to 13,551
_RS00080
AMB_RS00085: AMB_RS00085 - aspartate aminotransferase, at 13,864 to 15,063
_RS00085
Position (kb) 12
13
14 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 11.275 kb on + strand at 11.275 kb on + strand at 11.275 kb on + strand at 11.612 kb on + strand, within AMB_RS00075 at 11.627 kb on + strand, within AMB_RS00075 at 11.627 kb on + strand, within AMB_RS00075 at 11.951 kb on - strand at 11.974 kb on - strand at 11.974 kb on - strand at 12.081 kb on + strand at 12.237 kb on - strand at 12.255 kb on + strand at 12.255 kb on + strand at 12.407 kb on - strand, within AMB_RS00080 at 12.502 kb on + strand, within AMB_RS00080 at 12.711 kb on + strand, within AMB_RS00080 at 12.712 kb on - strand, within AMB_RS00080 at 13.225 kb on + strand, within AMB_RS00080 at 13.405 kb on + strand, within AMB_RS00080 at 13.429 kb on + strand at 13.429 kb on + strand at 13.549 kb on + strand at 13.628 kb on + strand at 13.628 kb on + strand at 13.628 kb on + strand at 13.629 kb on - strand at 13.629 kb on - strand at 13.629 kb on - strand at 13.629 kb on - strand at 13.811 kb on + strand at 13.816 kb on + strand at 13.816 kb on + strand at 13.816 kb on + strand at 13.819 kb on + strand at 13.819 kb on + strand at 13.819 kb on + strand at 13.819 kb on + strand at 14.053 kb on + strand, within AMB_RS00085 at 14.188 kb on + strand, within AMB_RS00085
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_3 remove 11,275 + -0.2 11,275 + -0.6 11,275 + -0.9 11,612 + AMB_RS00075 0.40 -2.6 11,627 + AMB_RS00075 0.44 +0.4 11,627 + AMB_RS00075 0.44 +0.4 11,951 - -0.4 11,974 - -0.5 11,974 - +0.7 12,081 + -0.5 12,237 - -0.5 12,255 + -3.0 12,255 + -1.0 12,407 - AMB_RS00080 0.15 +0.4 12,502 + AMB_RS00080 0.22 -0.5 12,711 + AMB_RS00080 0.38 -0.2 12,712 - AMB_RS00080 0.38 -0.2 13,225 + AMB_RS00080 0.76 -2.4 13,405 + AMB_RS00080 0.89 -1.3 13,429 + -1.0 13,429 + -1.2 13,549 + +0.6 13,628 + -0.8 13,628 + -0.1 13,628 + -0.5 13,629 - -0.9 13,629 - -0.1 13,629 - +0.0 13,629 - -2.6 13,811 + -0.4 13,816 + -0.1 13,816 + -0.1 13,816 + -0.6 13,819 + +0.3 13,819 + -0.2 13,819 + -0.2 13,819 + -0.3 14,053 + AMB_RS00085 0.16 -0.3 14,188 + AMB_RS00085 0.27 -0.2
Or see this region's nucleotide sequence