Experiment: L-Methionine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS22650 and ABIE40_RS22655 are separated by 12 nucleotides ABIE40_RS22655 and ABIE40_RS22660 overlap by 31 nucleotides ABIE40_RS22660 and ABIE40_RS22665 are separated by 170 nucleotides
ABIE40_RS22650: ABIE40_RS22650 - glutaredoxin-like protein NrdH, at 678,779 to 679,000
_RS22650
ABIE40_RS22655: ABIE40_RS22655 - class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI, at 679,013 to 679,423
_RS22655
ABIE40_RS22660: ABIE40_RS22660 - class 1b ribonucleoside-diphosphate reductase subunit alpha, at 679,393 to 681,552
_RS22660
ABIE40_RS22665: ABIE40_RS22665 - class 1b ribonucleoside-diphosphate reductase subunit beta, at 681,723 to 682,697
_RS22665
Position (kb)
679
680
681
682 Strain fitness (log2 ratio)
-1
0
1
2 at 678.625 kb on + strand at 678.625 kb on + strand at 678.625 kb on + strand at 678.625 kb on + strand at 678.625 kb on + strand at 678.625 kb on + strand at 678.625 kb on + strand at 678.626 kb on - strand at 679.155 kb on - strand, within ABIE40_RS22655 at 679.277 kb on + strand, within ABIE40_RS22655 at 679.323 kb on - strand, within ABIE40_RS22655 at 679.732 kb on + strand, within ABIE40_RS22660 at 679.732 kb on + strand, within ABIE40_RS22660 at 680.500 kb on + strand, within ABIE40_RS22660 at 680.501 kb on - strand, within ABIE40_RS22660 at 680.757 kb on + strand, within ABIE40_RS22660 at 680.758 kb on - strand, within ABIE40_RS22660 at 680.903 kb on - strand, within ABIE40_RS22660 at 680.914 kb on + strand, within ABIE40_RS22660 at 680.989 kb on + strand, within ABIE40_RS22660 at 680.990 kb on - strand, within ABIE40_RS22660 at 681.016 kb on + strand, within ABIE40_RS22660 at 681.144 kb on + strand, within ABIE40_RS22660 at 681.188 kb on - strand, within ABIE40_RS22660 at 681.481 kb on + strand at 681.481 kb on + strand at 681.481 kb on + strand at 681.564 kb on + strand at 681.564 kb on + strand at 681.564 kb on + strand at 681.564 kb on + strand at 681.565 kb on - strand at 681.723 kb on - strand at 682.102 kb on - strand, within ABIE40_RS22665 at 682.162 kb on - strand, within ABIE40_RS22665 at 682.162 kb on - strand, within ABIE40_RS22665 at 682.236 kb on + strand, within ABIE40_RS22665 at 682.249 kb on - strand, within ABIE40_RS22665 at 682.326 kb on + strand, within ABIE40_RS22665 at 682.327 kb on - strand, within ABIE40_RS22665 at 682.329 kb on + strand, within ABIE40_RS22665 at 682.330 kb on - strand, within ABIE40_RS22665 at 682.330 kb on - strand, within ABIE40_RS22665 at 682.504 kb on - strand, within ABIE40_RS22665
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (C) remove 678,625 + +0.0 678,625 + +0.8 678,625 + +0.2 678,625 + -0.5 678,625 + +0.1 678,625 + +0.8 678,625 + +0.7 678,626 - -0.8 679,155 - ABIE40_RS22655 0.35 +2.0 679,277 + ABIE40_RS22655 0.64 +2.0 679,323 - ABIE40_RS22655 0.75 -0.3 679,732 + ABIE40_RS22660 0.16 -0.3 679,732 + ABIE40_RS22660 0.16 +2.0 680,500 + ABIE40_RS22660 0.51 +0.2 680,501 - ABIE40_RS22660 0.51 -1.3 680,757 + ABIE40_RS22660 0.63 -0.6 680,758 - ABIE40_RS22660 0.63 -0.3 680,903 - ABIE40_RS22660 0.70 -1.0 680,914 + ABIE40_RS22660 0.70 -0.1 680,989 + ABIE40_RS22660 0.74 -0.6 680,990 - ABIE40_RS22660 0.74 -1.0 681,016 + ABIE40_RS22660 0.75 +2.0 681,144 + ABIE40_RS22660 0.81 -1.4 681,188 - ABIE40_RS22660 0.83 -1.7 681,481 + -0.9 681,481 + -0.6 681,481 + -0.8 681,564 + -0.3 681,564 + +2.6 681,564 + -0.5 681,564 + +0.6 681,565 - -0.8 681,723 - -0.3 682,102 - ABIE40_RS22665 0.39 +0.0 682,162 - ABIE40_RS22665 0.45 +1.0 682,162 - ABIE40_RS22665 0.45 -1.4 682,236 + ABIE40_RS22665 0.53 +1.0 682,249 - ABIE40_RS22665 0.54 -1.3 682,326 + ABIE40_RS22665 0.62 -1.0 682,327 - ABIE40_RS22665 0.62 +0.0 682,329 + ABIE40_RS22665 0.62 -0.3 682,330 - ABIE40_RS22665 0.62 +0.7 682,330 - ABIE40_RS22665 0.62 +0.7 682,504 - ABIE40_RS22665 0.80 +0.4
Or see this region's nucleotide sequence