Experiment: L-Methionine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS05665 and ABIE40_RS05670 are separated by 118 nucleotides ABIE40_RS05670 and ABIE40_RS05675 are separated by 229 nucleotides
ABIE40_RS05665: ABIE40_RS05665 - HAMP domain-containing sensor histidine kinase, at 1,108,426 to 1,109,829
_RS05665
ABIE40_RS05670: ABIE40_RS05670 - membrane protein, at 1,109,948 to 1,110,373
_RS05670
ABIE40_RS05675: ABIE40_RS05675 - c-type cytochrome biogenesis protein CcmI, at 1,110,603 to 1,111,736
_RS05675
Position (kb)
1109
1110
1111 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1109.303 kb on - strand, within ABIE40_RS05665 at 1109.855 kb on + strand at 1109.855 kb on + strand at 1109.856 kb on - strand at 1109.856 kb on - strand at 1109.856 kb on - strand at 1109.856 kb on - strand at 1109.856 kb on - strand at 1109.867 kb on + strand at 1109.867 kb on + strand at 1109.867 kb on + strand at 1109.868 kb on - strand at 1109.952 kb on + strand at 1109.953 kb on - strand at 1110.134 kb on - strand, within ABIE40_RS05670 at 1110.251 kb on + strand, within ABIE40_RS05670 at 1110.252 kb on - strand, within ABIE40_RS05670 at 1110.252 kb on - strand, within ABIE40_RS05670 at 1110.281 kb on + strand, within ABIE40_RS05670 at 1110.281 kb on + strand, within ABIE40_RS05670 at 1110.282 kb on - strand, within ABIE40_RS05670 at 1110.282 kb on - strand, within ABIE40_RS05670 at 1110.282 kb on - strand, within ABIE40_RS05670 at 1110.289 kb on + strand, within ABIE40_RS05670 at 1110.290 kb on - strand, within ABIE40_RS05670 at 1110.290 kb on - strand, within ABIE40_RS05670 at 1110.290 kb on - strand, within ABIE40_RS05670 at 1110.372 kb on - strand at 1110.372 kb on - strand at 1110.410 kb on - strand at 1110.410 kb on - strand at 1110.431 kb on + strand at 1110.431 kb on + strand at 1110.431 kb on + strand at 1110.431 kb on + strand at 1110.431 kb on + strand at 1110.432 kb on - strand at 1110.432 kb on - strand at 1110.432 kb on - strand at 1110.432 kb on - strand at 1110.432 kb on - strand at 1110.432 kb on - strand at 1110.432 kb on - strand at 1110.437 kb on + strand at 1110.438 kb on - strand at 1110.438 kb on - strand at 1110.438 kb on - strand at 1110.438 kb on - strand at 1110.438 kb on - strand at 1110.440 kb on + strand at 1110.440 kb on + strand at 1110.440 kb on + strand at 1110.441 kb on - strand at 1110.441 kb on - strand at 1110.441 kb on - strand at 1110.441 kb on - strand at 1110.507 kb on + strand at 1110.507 kb on + strand at 1110.603 kb on - strand at 1110.661 kb on - strand at 1110.721 kb on - strand, within ABIE40_RS05675 at 1110.721 kb on - strand, within ABIE40_RS05675 at 1110.721 kb on - strand, within ABIE40_RS05675 at 1110.789 kb on + strand, within ABIE40_RS05675 at 1110.789 kb on + strand, within ABIE40_RS05675 at 1110.927 kb on + strand, within ABIE40_RS05675 at 1110.928 kb on - strand, within ABIE40_RS05675 at 1110.928 kb on - strand, within ABIE40_RS05675 at 1111.083 kb on + strand, within ABIE40_RS05675 at 1111.084 kb on - strand, within ABIE40_RS05675 at 1111.084 kb on - strand, within ABIE40_RS05675 at 1111.084 kb on - strand, within ABIE40_RS05675 at 1111.084 kb on - strand, within ABIE40_RS05675 at 1111.282 kb on - strand, within ABIE40_RS05675
Per-strain Table
Position Strand Gene LocusTag Fraction L-Methionine (C) remove 1,109,303 - ABIE40_RS05665 0.62 -2.5 1,109,855 + -0.4 1,109,855 + -1.8 1,109,856 - +0.8 1,109,856 - +0.4 1,109,856 - -0.9 1,109,856 - +0.1 1,109,856 - -0.1 1,109,867 + -0.4 1,109,867 + +0.7 1,109,867 + -0.1 1,109,868 - -1.0 1,109,952 + -2.9 1,109,953 - -1.3 1,110,134 - ABIE40_RS05670 0.44 +1.5 1,110,251 + ABIE40_RS05670 0.71 -1.0 1,110,252 - ABIE40_RS05670 0.71 -1.5 1,110,252 - ABIE40_RS05670 0.71 -3.2 1,110,281 + ABIE40_RS05670 0.78 -2.2 1,110,281 + ABIE40_RS05670 0.78 -0.7 1,110,282 - ABIE40_RS05670 0.78 +0.1 1,110,282 - ABIE40_RS05670 0.78 -1.1 1,110,282 - ABIE40_RS05670 0.78 -1.0 1,110,289 + ABIE40_RS05670 0.80 -0.7 1,110,290 - ABIE40_RS05670 0.80 -1.3 1,110,290 - ABIE40_RS05670 0.80 -0.2 1,110,290 - ABIE40_RS05670 0.80 -1.8 1,110,372 - +0.8 1,110,372 - +0.6 1,110,410 - +0.1 1,110,410 - -0.2 1,110,431 + -0.6 1,110,431 + +1.1 1,110,431 + -0.1 1,110,431 + +0.3 1,110,431 + +0.7 1,110,432 - +0.3 1,110,432 - -0.7 1,110,432 - +1.6 1,110,432 - -1.1 1,110,432 - -0.3 1,110,432 - +0.1 1,110,432 - +0.4 1,110,437 + +0.2 1,110,438 - +3.2 1,110,438 - -0.0 1,110,438 - -0.0 1,110,438 - -1.7 1,110,438 - +0.4 1,110,440 + -0.5 1,110,440 + +0.2 1,110,440 + +0.5 1,110,441 - -1.3 1,110,441 - +1.5 1,110,441 - +0.2 1,110,441 - +0.0 1,110,507 + +1.1 1,110,507 + +1.1 1,110,603 - -1.3 1,110,661 - -0.5 1,110,721 - ABIE40_RS05675 0.10 -2.0 1,110,721 - ABIE40_RS05675 0.10 -1.2 1,110,721 - ABIE40_RS05675 0.10 -2.6 1,110,789 + ABIE40_RS05675 0.16 -0.4 1,110,789 + ABIE40_RS05675 0.16 -2.2 1,110,927 + ABIE40_RS05675 0.29 -0.2 1,110,928 - ABIE40_RS05675 0.29 -1.4 1,110,928 - ABIE40_RS05675 0.29 -2.0 1,111,083 + ABIE40_RS05675 0.42 +0.7 1,111,084 - ABIE40_RS05675 0.42 +0.7 1,111,084 - ABIE40_RS05675 0.42 -2.1 1,111,084 - ABIE40_RS05675 0.42 -0.3 1,111,084 - ABIE40_RS05675 0.42 -0.4 1,111,282 - ABIE40_RS05675 0.60 -1.2
Or see this region's nucleotide sequence