Experiment: D-Galacturonic Acid (C) MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfI and b2490 overlap by 8 nucleotides b2490 and hyfR are separated by 29 nucleotides hyfR and focB are separated by 21 nucleotides focB and yfgO are separated by 37 nucleotides
b2489: hyfI - hydrogenase 4, Fe-S subunit (NCBI), at 2,608,728 to 2,609,486
hyfI
b2490: b2490 - putative protein processing element (VIMSS), at 2,609,479 to 2,609,892
b2490
b2491: hyfR - putative 2-component regulator, interaction with sigma 54 (VIMSS), at 2,609,922 to 2,611,934
hyfR
b2492: focB - predicted formate transporter (NCBI), at 2,611,956 to 2,612,804
focB
b2493: yfgO - predicted inner membrane protein (NCBI), at 2,612,842 to 2,613,903
yfgO
Position (kb)
2609
2610
2611
2612 Strain fitness (log2 ratio)
-1
0
1 at 2609.013 kb on + strand, within hyfI at 2609.013 kb on + strand, within hyfI at 2609.095 kb on - strand, within hyfI at 2609.097 kb on + strand, within hyfI at 2609.290 kb on - strand, within hyfI at 2609.329 kb on + strand, within hyfI at 2609.329 kb on + strand, within hyfI at 2609.393 kb on - strand, within hyfI at 2609.393 kb on - strand, within hyfI at 2609.480 kb on + strand at 2609.482 kb on + strand at 2609.490 kb on - strand at 2609.490 kb on - strand at 2609.576 kb on - strand, within b2490 at 2609.576 kb on - strand, within b2490 at 2609.595 kb on - strand, within b2490 at 2609.671 kb on - strand, within b2490 at 2610.075 kb on - strand at 2610.080 kb on - strand at 2610.149 kb on - strand, within hyfR at 2610.261 kb on + strand, within hyfR at 2610.284 kb on - strand, within hyfR at 2610.286 kb on - strand, within hyfR at 2610.406 kb on + strand, within hyfR at 2610.411 kb on + strand, within hyfR at 2610.555 kb on + strand, within hyfR at 2610.555 kb on + strand, within hyfR at 2610.733 kb on - strand, within hyfR at 2610.768 kb on - strand, within hyfR at 2610.768 kb on - strand, within hyfR at 2610.852 kb on - strand, within hyfR at 2610.852 kb on - strand, within hyfR at 2611.065 kb on + strand, within hyfR at 2611.090 kb on + strand, within hyfR at 2611.139 kb on - strand, within hyfR at 2611.139 kb on - strand, within hyfR at 2611.289 kb on + strand, within hyfR at 2611.392 kb on - strand, within hyfR at 2611.599 kb on - strand, within hyfR at 2611.599 kb on - strand, within hyfR at 2611.829 kb on - strand at 2611.950 kb on + strand at 2612.067 kb on - strand, within focB at 2612.067 kb on - strand, within focB at 2612.133 kb on - strand, within focB at 2612.145 kb on + strand, within focB at 2612.214 kb on + strand, within focB at 2612.214 kb on + strand, within focB at 2612.217 kb on - strand, within focB at 2612.217 kb on - strand, within focB at 2612.397 kb on + strand, within focB at 2612.425 kb on + strand, within focB at 2612.497 kb on - strand, within focB at 2612.579 kb on + strand, within focB at 2612.579 kb on + strand, within focB at 2612.589 kb on - strand, within focB at 2612.690 kb on - strand, within focB at 2612.690 kb on - strand, within focB at 2612.720 kb on - strand at 2612.720 kb on - strand at 2612.749 kb on + strand at 2612.749 kb on + strand at 2612.764 kb on + strand at 2612.764 kb on + strand at 2612.845 kb on - strand at 2612.845 kb on - strand at 2612.875 kb on - strand at 2612.875 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galacturonic Acid (C) MOPS remove 2,609,013 + hyfI b2489 0.38 +0.5 2,609,013 + hyfI b2489 0.38 +0.1 2,609,095 - hyfI b2489 0.48 -0.2 2,609,097 + hyfI b2489 0.49 -0.2 2,609,290 - hyfI b2489 0.74 -0.1 2,609,329 + hyfI b2489 0.79 +0.3 2,609,329 + hyfI b2489 0.79 -0.1 2,609,393 - hyfI b2489 0.88 -0.1 2,609,393 - hyfI b2489 0.88 +0.6 2,609,480 + -0.2 2,609,482 + +0.2 2,609,490 - -0.9 2,609,490 - -0.9 2,609,576 - b2490 b2490 0.23 +0.5 2,609,576 - b2490 b2490 0.23 -0.3 2,609,595 - b2490 b2490 0.28 -0.4 2,609,671 - b2490 b2490 0.46 -0.3 2,610,075 - -0.3 2,610,080 - -0.3 2,610,149 - hyfR b2491 0.11 -1.6 2,610,261 + hyfR b2491 0.17 -0.3 2,610,284 - hyfR b2491 0.18 -0.0 2,610,286 - hyfR b2491 0.18 +0.0 2,610,406 + hyfR b2491 0.24 +0.9 2,610,411 + hyfR b2491 0.24 +1.1 2,610,555 + hyfR b2491 0.31 +0.1 2,610,555 + hyfR b2491 0.31 +0.8 2,610,733 - hyfR b2491 0.40 -0.8 2,610,768 - hyfR b2491 0.42 -0.2 2,610,768 - hyfR b2491 0.42 +0.2 2,610,852 - hyfR b2491 0.46 -0.3 2,610,852 - hyfR b2491 0.46 +0.1 2,611,065 + hyfR b2491 0.57 +0.7 2,611,090 + hyfR b2491 0.58 +0.2 2,611,139 - hyfR b2491 0.60 +0.7 2,611,139 - hyfR b2491 0.60 -0.1 2,611,289 + hyfR b2491 0.68 -0.1 2,611,392 - hyfR b2491 0.73 +0.3 2,611,599 - hyfR b2491 0.83 +0.1 2,611,599 - hyfR b2491 0.83 +1.0 2,611,829 - +0.1 2,611,950 + -0.2 2,612,067 - focB b2492 0.13 +0.3 2,612,067 - focB b2492 0.13 +0.1 2,612,133 - focB b2492 0.21 -0.0 2,612,145 + focB b2492 0.22 -0.5 2,612,214 + focB b2492 0.30 +0.6 2,612,214 + focB b2492 0.30 -0.5 2,612,217 - focB b2492 0.31 -0.9 2,612,217 - focB b2492 0.31 -0.0 2,612,397 + focB b2492 0.52 +0.3 2,612,425 + focB b2492 0.55 +0.3 2,612,497 - focB b2492 0.64 +0.5 2,612,579 + focB b2492 0.73 -0.8 2,612,579 + focB b2492 0.73 +0.8 2,612,589 - focB b2492 0.75 +0.3 2,612,690 - focB b2492 0.86 -0.5 2,612,690 - focB b2492 0.86 +0.3 2,612,720 - -0.2 2,612,720 - -0.9 2,612,749 + +0.1 2,612,749 + -1.1 2,612,764 + -1.1 2,612,764 + -0.1 2,612,845 - +0.5 2,612,845 - -0.1 2,612,875 - -0.1 2,612,875 - +0.9
Or see this region's nucleotide sequence