Experiment: CPG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt UW163_RS11160 and UW163_RS11165 are separated by 86 nucleotides UW163_RS11165 and UW163_RS11170 are separated by 72 nucleotides UW163_RS11170 and UW163_RS11175 are separated by 97 nucleotides
UW163_RS11160: UW163_RS11160 - bifunctional tetrahydrofolate synthase/dihydrofolate synthase, at 2,412,344 to 2,413,654
_RS11160
UW163_RS11165: UW163_RS11165 - SPOR domain-containing protein, at 2,413,741 to 2,414,580
_RS11165
UW163_RS11170: UW163_RS11170 - colicin V biosynthesis protein, at 2,414,653 to 2,415,150
_RS11170
UW163_RS11175: UW163_RS11175 - amidophosphoribosyltransferase, at 2,415,248 to 2,416,783
_RS11175
Position (kb)
2414
2415
2416 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2414.674 kb on + strand at 2414.782 kb on + strand, within UW163_RS11170 at 2414.783 kb on - strand, within UW163_RS11170 at 2414.784 kb on + strand, within UW163_RS11170 at 2414.784 kb on + strand, within UW163_RS11170 at 2414.784 kb on + strand, within UW163_RS11170 at 2414.867 kb on + strand, within UW163_RS11170 at 2414.868 kb on - strand, within UW163_RS11170 at 2415.145 kb on + strand at 2415.374 kb on + strand at 2415.375 kb on - strand at 2415.375 kb on - strand at 2415.375 kb on - strand at 2415.513 kb on - strand, within UW163_RS11175 at 2416.013 kb on + strand, within UW163_RS11175
Per-strain Table
Position Strand Gene LocusTag Fraction CPG remove 2,414,674 + +0.2 2,414,782 + UW163_RS11170 0.26 -1.0 2,414,783 - UW163_RS11170 0.26 -0.5 2,414,784 + UW163_RS11170 0.26 -1.4 2,414,784 + UW163_RS11170 0.26 -2.0 2,414,784 + UW163_RS11170 0.26 +0.3 2,414,867 + UW163_RS11170 0.43 -0.3 2,414,868 - UW163_RS11170 0.43 -3.0 2,415,145 + -0.1 2,415,374 + -0.6 2,415,375 - +0.2 2,415,375 - +0.8 2,415,375 - -0.6 2,415,513 - UW163_RS11175 0.17 -2.2 2,416,013 + UW163_RS11175 0.50 -0.6
Or see this region's nucleotide sequence