Experiment: CPG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt UW163_RS03010 and UW163_RS03015 are separated by 58 nucleotides UW163_RS03015 and UW163_RS03020 are separated by 101 nucleotides UW163_RS03020 and UW163_RS03025 are separated by 113 nucleotides UW163_RS03025 and UW163_RS03030 overlap by 1 nucleotides
UW163_RS03010: UW163_RS03010 - arginine--tRNA ligase, at 621,466 to 623,268
_RS03010
UW163_RS03015: UW163_RS03015 - SPOR domain-containing protein, at 623,327 to 624,049
_RS03015
UW163_RS03020: UW163_RS03020 - thiol:disulfide interchange protein DsbA/DsbL, at 624,151 to 624,807
_RS03020
UW163_RS03025: UW163_RS03025 - short-chain dehydrogenase, at 624,921 to 625,721
_RS03025
UW163_RS03030: UW163_RS03030 - vitamin b12 transporter subunit: periplasmic-binding protein of ABC superfamily, at 625,721 to 626,566
_RS03030
Position (kb) 624
625 Strain fitness (log2 ratio) -2
-1
0
1 at 623.267 kb on - strand at 623.300 kb on - strand at 623.302 kb on - strand at 623.438 kb on + strand, within UW163_RS03015 at 623.438 kb on + strand, within UW163_RS03015 at 623.438 kb on + strand, within UW163_RS03015 at 623.439 kb on - strand, within UW163_RS03015 at 624.442 kb on + strand, within UW163_RS03020 at 624.443 kb on - strand, within UW163_RS03020 at 624.443 kb on - strand, within UW163_RS03020 at 624.536 kb on - strand, within UW163_RS03020 at 624.536 kb on - strand, within UW163_RS03020 at 624.616 kb on + strand, within UW163_RS03020 at 624.642 kb on + strand, within UW163_RS03020 at 624.715 kb on + strand, within UW163_RS03020 at 624.715 kb on + strand, within UW163_RS03020 at 624.715 kb on + strand, within UW163_RS03020 at 624.715 kb on + strand, within UW163_RS03020 at 624.772 kb on + strand at 625.387 kb on - strand, within UW163_RS03025 at 625.492 kb on - strand, within UW163_RS03025 at 625.665 kb on + strand at 625.665 kb on + strand at 625.665 kb on + strand at 625.666 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CPG remove 623,267 - -0.4 623,300 - +0.3 623,302 - -1.9 623,438 + UW163_RS03015 0.15 -2.6 623,438 + UW163_RS03015 0.15 -1.3 623,438 + UW163_RS03015 0.15 -1.4 623,439 - UW163_RS03015 0.15 +0.2 624,442 + UW163_RS03020 0.44 -2.7 624,443 - UW163_RS03020 0.44 -2.1 624,443 - UW163_RS03020 0.44 -0.4 624,536 - UW163_RS03020 0.59 -1.1 624,536 - UW163_RS03020 0.59 +0.5 624,616 + UW163_RS03020 0.71 -0.1 624,642 + UW163_RS03020 0.75 -1.0 624,715 + UW163_RS03020 0.86 -0.0 624,715 + UW163_RS03020 0.86 +1.0 624,715 + UW163_RS03020 0.86 -0.4 624,715 + UW163_RS03020 0.86 -2.5 624,772 + +0.3 625,387 - UW163_RS03025 0.58 +0.0 625,492 - UW163_RS03025 0.71 -0.4 625,665 + -0.6 625,665 + -0.3 625,665 + +0.5 625,666 - -0.3
Or see this region's nucleotide sequence