Experiment: CPG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt UW163_RS02370 and UW163_RS02375 are separated by 43 nucleotides UW163_RS02375 and UW163_RS02380 are separated by 10 nucleotides UW163_RS02380 and UW163_RS24360 overlap by 6 nucleotides UW163_RS24360 and UW163_RS02385 overlap by 4 nucleotides
UW163_RS02370: UW163_RS02370 - glutamine amidotransferase, at 499,353 to 499,931
_RS02370
UW163_RS02375: UW163_RS02375 - aryl-sulfate sulfotransferase, at 499,975 to 500,379
_RS02375
UW163_RS02380: UW163_RS02380 - potassium transporter, at 500,390 to 502,369
_RS02380
UW163_RS24360: UW163_RS24360 - hypothetical protein, at 502,364 to 502,675
_RS24360
UW163_RS02385: UW163_RS02385 - KpsF/GutQ family sugar-phosphate isomerase, at 502,672 to 503,655
_RS02385
Position (kb) 500
501
502
503 Strain fitness (log2 ratio) -1
0
1 at 499.524 kb on + strand, within UW163_RS02370 at 499.524 kb on + strand, within UW163_RS02370 at 499.525 kb on - strand at 499.590 kb on + strand, within UW163_RS02370 at 499.925 kb on - strand at 500.377 kb on + strand at 500.664 kb on + strand, within UW163_RS02380 at 500.683 kb on - strand, within UW163_RS02380 at 500.997 kb on + strand, within UW163_RS02380 at 500.997 kb on + strand, within UW163_RS02380 at 500.998 kb on - strand, within UW163_RS02380 at 501.063 kb on + strand, within UW163_RS02380 at 501.064 kb on - strand, within UW163_RS02380 at 502.203 kb on + strand at 502.272 kb on + strand at 502.658 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CPG remove 499,524 + UW163_RS02370 0.30 +0.2 499,524 + UW163_RS02370 0.30 +0.0 499,525 - +0.8 499,590 + UW163_RS02370 0.41 +0.2 499,925 - -0.1 500,377 + +0.1 500,664 + UW163_RS02380 0.14 +0.0 500,683 - UW163_RS02380 0.15 -0.0 500,997 + UW163_RS02380 0.31 +0.5 500,997 + UW163_RS02380 0.31 +0.3 500,998 - UW163_RS02380 0.31 +0.4 501,063 + UW163_RS02380 0.34 -0.0 501,064 - UW163_RS02380 0.34 +1.3 502,203 + +0.0 502,272 + +0.6 502,658 + +0.0
Or see this region's nucleotide sequence