Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11010 and AMB_RS24475 are separated by 491 nucleotides AMB_RS24475 and AMB_RS11015 are separated by 13 nucleotides AMB_RS11015 and AMB_RS24480 overlap by 86 nucleotides
AMB_RS11010: AMB_RS11010 - two-component sensor histidine kinase, at 2,360,324 to 2,361,823
_RS11010
AMB_RS24475: AMB_RS24475 - hypothetical protein, at 2,362,315 to 2,363,655
_RS24475
AMB_RS11015: AMB_RS11015 - XRE family transcriptional regulator, at 2,363,669 to 2,364,274
_RS11015
AMB_RS24480: AMB_RS24480 - transcriptional regulator, at 2,364,189 to 2,364,686
_RS24480
Position (kb)
2362
2363
2364 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2361.387 kb on - strand, within AMB_RS11010 at 2361.387 kb on - strand, within AMB_RS11010 at 2361.396 kb on + strand, within AMB_RS11010 at 2361.397 kb on - strand, within AMB_RS11010 at 2361.397 kb on - strand, within AMB_RS11010 at 2361.522 kb on - strand, within AMB_RS11010 at 2361.522 kb on - strand, within AMB_RS11010 at 2361.522 kb on - strand, within AMB_RS11010 at 2361.752 kb on + strand at 2361.753 kb on - strand at 2361.759 kb on + strand at 2361.759 kb on + strand at 2361.759 kb on + strand at 2361.759 kb on + strand at 2361.760 kb on - strand at 2361.760 kb on - strand at 2361.760 kb on - strand at 2361.760 kb on - strand at 2361.760 kb on - strand at 2361.760 kb on - strand at 2361.760 kb on - strand at 2361.877 kb on + strand at 2361.877 kb on + strand at 2361.878 kb on - strand at 2361.878 kb on - strand at 2361.956 kb on - strand at 2361.999 kb on + strand at 2362.000 kb on - strand at 2362.010 kb on + strand at 2362.011 kb on - strand at 2362.021 kb on - strand at 2362.079 kb on + strand at 2362.080 kb on - strand at 2362.080 kb on - strand at 2362.084 kb on + strand at 2362.085 kb on - strand at 2362.107 kb on - strand at 2362.109 kb on - strand at 2362.115 kb on - strand at 2362.197 kb on + strand at 2362.197 kb on + strand at 2362.197 kb on + strand at 2362.198 kb on - strand at 2362.200 kb on + strand at 2362.201 kb on - strand at 2362.232 kb on - strand at 2362.234 kb on - strand at 2362.248 kb on - strand at 2362.248 kb on - strand at 2362.439 kb on + strand at 2362.444 kb on - strand at 2362.446 kb on + strand at 2362.447 kb on - strand at 2362.503 kb on + strand, within AMB_RS24475 at 2362.504 kb on - strand, within AMB_RS24475 at 2362.504 kb on - strand, within AMB_RS24475 at 2362.504 kb on - strand, within AMB_RS24475 at 2362.597 kb on - strand, within AMB_RS24475 at 2362.859 kb on + strand, within AMB_RS24475 at 2362.860 kb on - strand, within AMB_RS24475 at 2362.954 kb on + strand, within AMB_RS24475 at 2363.107 kb on - strand, within AMB_RS24475 at 2363.141 kb on - strand, within AMB_RS24475 at 2363.153 kb on - strand, within AMB_RS24475 at 2363.421 kb on - strand, within AMB_RS24475 at 2363.478 kb on + strand, within AMB_RS24475 at 2363.478 kb on + strand, within AMB_RS24475 at 2363.479 kb on - strand, within AMB_RS24475 at 2363.667 kb on + strand at 2363.671 kb on - strand at 2363.671 kb on - strand at 2363.910 kb on - strand, within AMB_RS11015 at 2364.163 kb on + strand, within AMB_RS11015 at 2364.163 kb on + strand, within AMB_RS11015 at 2364.163 kb on + strand, within AMB_RS11015 at 2364.163 kb on + strand, within AMB_RS11015 at 2364.163 kb on + strand, within AMB_RS11015 at 2364.164 kb on - strand, within AMB_RS11015 at 2364.190 kb on + strand at 2364.223 kb on + strand at 2364.355 kb on + strand, within AMB_RS24480 at 2364.355 kb on + strand, within AMB_RS24480 at 2364.356 kb on - strand, within AMB_RS24480 at 2364.356 kb on - strand, within AMB_RS24480 at 2364.356 kb on - strand, within AMB_RS24480 at 2364.414 kb on + strand, within AMB_RS24480 at 2364.414 kb on + strand, within AMB_RS24480 at 2364.415 kb on - strand, within AMB_RS24480 at 2364.421 kb on - strand, within AMB_RS24480 at 2364.421 kb on - strand, within AMB_RS24480 at 2364.513 kb on - strand, within AMB_RS24480 at 2364.584 kb on - strand, within AMB_RS24480 at 2364.640 kb on + strand at 2364.640 kb on + strand at 2364.641 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 2,361,387 - AMB_RS11010 0.71 -0.2 2,361,387 - AMB_RS11010 0.71 +0.8 2,361,396 + AMB_RS11010 0.71 -0.2 2,361,397 - AMB_RS11010 0.72 -0.1 2,361,397 - AMB_RS11010 0.72 +0.3 2,361,522 - AMB_RS11010 0.80 -0.1 2,361,522 - AMB_RS11010 0.80 +0.6 2,361,522 - AMB_RS11010 0.80 -0.2 2,361,752 + -0.4 2,361,753 - +1.8 2,361,759 + +0.2 2,361,759 + -0.4 2,361,759 + +0.1 2,361,759 + -1.4 2,361,760 - -0.7 2,361,760 - -0.0 2,361,760 - -0.6 2,361,760 - -0.1 2,361,760 - -0.1 2,361,760 - -1.9 2,361,760 - -0.2 2,361,877 + -0.2 2,361,877 + +0.5 2,361,878 - -0.2 2,361,878 - +0.3 2,361,956 - -1.0 2,361,999 + +0.3 2,362,000 - +1.1 2,362,010 + -0.2 2,362,011 - -0.3 2,362,021 - -0.5 2,362,079 + -0.3 2,362,080 - -0.1 2,362,080 - -0.7 2,362,084 + -0.4 2,362,085 - +0.3 2,362,107 - -0.5 2,362,109 - +0.1 2,362,115 - -1.1 2,362,197 + -0.7 2,362,197 + +0.5 2,362,197 + -0.4 2,362,198 - -0.2 2,362,200 + +0.2 2,362,201 - -2.4 2,362,232 - +0.2 2,362,234 - +0.3 2,362,248 - +0.1 2,362,248 - -0.7 2,362,439 + -0.1 2,362,444 - -0.8 2,362,446 + -0.9 2,362,447 - -2.6 2,362,503 + AMB_RS24475 0.14 -2.0 2,362,504 - AMB_RS24475 0.14 +0.0 2,362,504 - AMB_RS24475 0.14 -0.6 2,362,504 - AMB_RS24475 0.14 -0.2 2,362,597 - AMB_RS24475 0.21 +0.0 2,362,859 + AMB_RS24475 0.41 -0.5 2,362,860 - AMB_RS24475 0.41 -0.3 2,362,954 + AMB_RS24475 0.48 -1.0 2,363,107 - AMB_RS24475 0.59 +0.0 2,363,141 - AMB_RS24475 0.62 -0.6 2,363,153 - AMB_RS24475 0.62 -0.1 2,363,421 - AMB_RS24475 0.82 -0.6 2,363,478 + AMB_RS24475 0.87 -1.6 2,363,478 + AMB_RS24475 0.87 +0.2 2,363,479 - AMB_RS24475 0.87 -1.9 2,363,667 + -1.1 2,363,671 - -0.7 2,363,671 - -0.3 2,363,910 - AMB_RS11015 0.40 -0.2 2,364,163 + AMB_RS11015 0.82 -1.2 2,364,163 + AMB_RS11015 0.82 -0.1 2,364,163 + AMB_RS11015 0.82 +0.2 2,364,163 + AMB_RS11015 0.82 -0.7 2,364,163 + AMB_RS11015 0.82 -0.8 2,364,164 - AMB_RS11015 0.82 -1.0 2,364,190 + -0.2 2,364,223 + -1.1 2,364,355 + AMB_RS24480 0.33 -0.8 2,364,355 + AMB_RS24480 0.33 -0.3 2,364,356 - AMB_RS24480 0.34 -0.6 2,364,356 - AMB_RS24480 0.34 -0.2 2,364,356 - AMB_RS24480 0.34 -0.4 2,364,414 + AMB_RS24480 0.45 -1.1 2,364,414 + AMB_RS24480 0.45 -0.2 2,364,415 - AMB_RS24480 0.45 -1.1 2,364,421 - AMB_RS24480 0.47 +0.3 2,364,421 - AMB_RS24480 0.47 -0.2 2,364,513 - AMB_RS24480 0.65 -2.6 2,364,584 - AMB_RS24480 0.79 -0.4 2,364,640 + -0.1 2,364,640 + -0.2 2,364,641 - -0.5
Or see this region's nucleotide sequence