Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS08830 and AMB_RS08835 are separated by 4 nucleotides AMB_RS08835 and AMB_RS08840 are separated by 72 nucleotides AMB_RS08840 and AMB_RS23330 are separated by 54 nucleotides AMB_RS23330 and AMB_RS08850 are separated by 5 nucleotides AMB_RS08850 and AMB_RS08855 overlap by 4 nucleotides AMB_RS08855 and AMB_RS08860 are separated by 2 nucleotides AMB_RS08860 and AMB_RS24340 are separated by 39 nucleotides
AMB_RS08830: AMB_RS08830 - hypothetical protein, at 1,891,574 to 1,891,963
_RS08830
AMB_RS08835: AMB_RS08835 - hypothetical protein, at 1,891,968 to 1,892,171
_RS08835
AMB_RS08840: AMB_RS08840 - transcriptional regulator, at 1,892,244 to 1,892,747
_RS08840
AMB_RS23330: AMB_RS23330 - hypothetical protein, at 1,892,802 to 1,893,638
_RS23330
AMB_RS08850: AMB_RS08850 - hypothetical protein, at 1,893,644 to 1,893,922
_RS08850
AMB_RS08855: AMB_RS08855 - hypothetical protein, at 1,893,919 to 1,894,128
_RS08855
AMB_RS08860: AMB_RS08860 - hypothetical protein, at 1,894,131 to 1,894,460
_RS08860
AMB_RS24340: AMB_RS24340 - DNA cytosine methyltransferase, at 1,894,500 to 1,895,732
_RS24340
Position (kb) 1892
1893
1894 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1891.913 kb on - strand, within AMB_RS08830 at 1891.999 kb on - strand, within AMB_RS08835 at 1892.170 kb on - strand at 1892.170 kb on - strand at 1892.170 kb on - strand at 1892.170 kb on - strand at 1892.249 kb on + strand at 1892.249 kb on + strand at 1892.249 kb on + strand at 1892.249 kb on + strand at 1892.249 kb on + strand at 1892.249 kb on + strand at 1892.249 kb on + strand at 1892.249 kb on + strand at 1892.250 kb on - strand at 1892.250 kb on - strand at 1892.250 kb on - strand at 1892.250 kb on - strand at 1892.250 kb on - strand at 1892.250 kb on - strand at 1892.250 kb on - strand at 1892.250 kb on - strand at 1892.250 kb on - strand at 1892.360 kb on + strand, within AMB_RS08840 at 1892.361 kb on - strand, within AMB_RS08840 at 1892.446 kb on - strand, within AMB_RS08840 at 1892.446 kb on - strand, within AMB_RS08840 at 1892.446 kb on - strand, within AMB_RS08840 at 1892.446 kb on - strand, within AMB_RS08840 at 1892.521 kb on - strand, within AMB_RS08840 at 1892.521 kb on - strand, within AMB_RS08840 at 1892.701 kb on - strand at 1892.701 kb on - strand at 1892.701 kb on - strand at 1892.701 kb on - strand at 1892.701 kb on - strand at 1892.701 kb on - strand at 1892.787 kb on - strand at 1892.889 kb on + strand, within AMB_RS23330 at 1892.890 kb on - strand, within AMB_RS23330 at 1893.150 kb on + strand, within AMB_RS23330 at 1893.150 kb on + strand, within AMB_RS23330 at 1893.150 kb on + strand, within AMB_RS23330 at 1893.150 kb on + strand, within AMB_RS23330 at 1893.151 kb on - strand, within AMB_RS23330 at 1893.151 kb on - strand, within AMB_RS23330 at 1893.243 kb on + strand, within AMB_RS23330 at 1893.244 kb on - strand, within AMB_RS23330 at 1893.440 kb on + strand, within AMB_RS23330 at 1893.440 kb on + strand, within AMB_RS23330 at 1893.636 kb on + strand at 1893.637 kb on - strand at 1894.027 kb on + strand, within AMB_RS08855 at 1894.027 kb on + strand, within AMB_RS08855 at 1894.027 kb on + strand, within AMB_RS08855 at 1894.027 kb on + strand, within AMB_RS08855 at 1894.028 kb on - strand, within AMB_RS08855 at 1894.028 kb on - strand, within AMB_RS08855 at 1894.028 kb on - strand, within AMB_RS08855 at 1894.028 kb on - strand, within AMB_RS08855 at 1894.052 kb on - strand, within AMB_RS08855 at 1894.187 kb on + strand, within AMB_RS08860 at 1894.519 kb on + strand at 1894.519 kb on + strand at 1894.519 kb on + strand at 1894.520 kb on - strand at 1894.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 1,891,913 - AMB_RS08830 0.87 -0.5 1,891,999 - AMB_RS08835 0.15 -0.4 1,892,170 - +0.1 1,892,170 - +0.0 1,892,170 - -0.2 1,892,170 - -2.1 1,892,249 + -2.5 1,892,249 + +0.5 1,892,249 + -0.3 1,892,249 + -1.5 1,892,249 + +0.1 1,892,249 + -0.8 1,892,249 + +0.3 1,892,249 + +0.1 1,892,250 - +0.1 1,892,250 - -0.3 1,892,250 - -0.1 1,892,250 - -2.4 1,892,250 - -1.1 1,892,250 - +0.5 1,892,250 - +1.6 1,892,250 - -2.0 1,892,250 - +1.1 1,892,360 + AMB_RS08840 0.23 +0.7 1,892,361 - AMB_RS08840 0.23 -0.7 1,892,446 - AMB_RS08840 0.40 -0.5 1,892,446 - AMB_RS08840 0.40 -3.6 1,892,446 - AMB_RS08840 0.40 +0.0 1,892,446 - AMB_RS08840 0.40 -0.6 1,892,521 - AMB_RS08840 0.55 -0.4 1,892,521 - AMB_RS08840 0.55 +0.9 1,892,701 - -0.8 1,892,701 - -0.2 1,892,701 - -0.3 1,892,701 - -0.5 1,892,701 - -1.2 1,892,701 - +0.4 1,892,787 - -0.3 1,892,889 + AMB_RS23330 0.10 -0.0 1,892,890 - AMB_RS23330 0.11 -1.3 1,893,150 + AMB_RS23330 0.42 -1.1 1,893,150 + AMB_RS23330 0.42 -0.3 1,893,150 + AMB_RS23330 0.42 -0.2 1,893,150 + AMB_RS23330 0.42 -1.1 1,893,151 - AMB_RS23330 0.42 -0.3 1,893,151 - AMB_RS23330 0.42 -0.6 1,893,243 + AMB_RS23330 0.53 -0.2 1,893,244 - AMB_RS23330 0.53 -0.8 1,893,440 + AMB_RS23330 0.76 -0.1 1,893,440 + AMB_RS23330 0.76 -3.5 1,893,636 + -1.4 1,893,637 - -0.4 1,894,027 + AMB_RS08855 0.51 -0.3 1,894,027 + AMB_RS08855 0.51 +1.6 1,894,027 + AMB_RS08855 0.51 -1.6 1,894,027 + AMB_RS08855 0.51 -0.5 1,894,028 - AMB_RS08855 0.52 -0.1 1,894,028 - AMB_RS08855 0.52 -1.8 1,894,028 - AMB_RS08855 0.52 +0.8 1,894,028 - AMB_RS08855 0.52 -0.5 1,894,052 - AMB_RS08855 0.63 -0.1 1,894,187 + AMB_RS08860 0.17 +0.2 1,894,519 + +0.1 1,894,519 + -1.1 1,894,519 + -0.5 1,894,520 - -1.2 1,894,520 - -0.1
Or see this region's nucleotide sequence