Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23050 and AMB_RS23055 overlap by 4 nucleotides AMB_RS23055 and AMB_RS23060 are separated by 8 nucleotides
AMB_RS23050: AMB_RS23050 - quinone oxidoreductase, at 4,963,015 to 4,963,992
_RS23050
AMB_RS23055: AMB_RS23055 - methylated-DNA--protein-cysteine methyltransferase, at 4,963,989 to 4,964,441
_RS23055
AMB_RS23060: AMB_RS23060 - thioredoxin domain-containing protein, at 4,964,450 to 4,966,465
_RS23060
Position (kb)
4963
4964
4965 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4962.996 kb on + strand at 4962.996 kb on + strand at 4963.094 kb on + strand at 4963.094 kb on + strand at 4963.094 kb on + strand at 4963.094 kb on + strand at 4963.095 kb on - strand at 4963.095 kb on - strand at 4963.095 kb on - strand at 4963.095 kb on - strand at 4963.095 kb on - strand at 4963.095 kb on - strand at 4963.188 kb on - strand, within AMB_RS23050 at 4963.188 kb on - strand, within AMB_RS23050 at 4963.390 kb on + strand, within AMB_RS23050 at 4963.390 kb on + strand, within AMB_RS23050 at 4963.418 kb on + strand, within AMB_RS23050 at 4963.419 kb on - strand, within AMB_RS23050 at 4963.425 kb on + strand, within AMB_RS23050 at 4963.425 kb on + strand, within AMB_RS23050 at 4963.425 kb on + strand, within AMB_RS23050 at 4963.425 kb on + strand, within AMB_RS23050 at 4963.425 kb on + strand, within AMB_RS23050 at 4963.425 kb on + strand, within AMB_RS23050 at 4963.426 kb on - strand, within AMB_RS23050 at 4963.426 kb on - strand, within AMB_RS23050 at 4963.426 kb on - strand, within AMB_RS23050 at 4963.426 kb on - strand, within AMB_RS23050 at 4963.426 kb on - strand, within AMB_RS23050 at 4963.586 kb on + strand, within AMB_RS23050 at 4963.587 kb on - strand, within AMB_RS23050 at 4963.604 kb on + strand, within AMB_RS23050 at 4963.604 kb on + strand, within AMB_RS23050 at 4963.709 kb on + strand, within AMB_RS23050 at 4963.709 kb on + strand, within AMB_RS23050 at 4963.709 kb on + strand, within AMB_RS23050 at 4963.710 kb on - strand, within AMB_RS23050 at 4963.733 kb on + strand, within AMB_RS23050 at 4963.733 kb on + strand, within AMB_RS23050 at 4963.733 kb on + strand, within AMB_RS23050 at 4963.835 kb on + strand, within AMB_RS23050 at 4963.865 kb on + strand, within AMB_RS23050 at 4963.865 kb on + strand, within AMB_RS23050 at 4963.865 kb on + strand, within AMB_RS23050 at 4963.865 kb on + strand, within AMB_RS23050 at 4963.865 kb on + strand, within AMB_RS23050 at 4963.866 kb on - strand, within AMB_RS23050 at 4964.056 kb on + strand, within AMB_RS23055 at 4964.182 kb on + strand, within AMB_RS23055 at 4964.183 kb on - strand, within AMB_RS23055 at 4964.296 kb on + strand, within AMB_RS23055 at 4964.297 kb on - strand, within AMB_RS23055 at 4964.451 kb on + strand at 4964.524 kb on + strand at 4964.524 kb on + strand at 4964.524 kb on + strand at 4964.524 kb on + strand at 4964.525 kb on - strand at 4964.526 kb on + strand at 4964.526 kb on + strand at 4964.526 kb on + strand at 4964.716 kb on + strand, within AMB_RS23060 at 4965.265 kb on + strand, within AMB_RS23060 at 4965.266 kb on - strand, within AMB_RS23060 at 4965.266 kb on - strand, within AMB_RS23060 at 4965.415 kb on - strand, within AMB_RS23060
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 4,962,996 + -1.5 4,962,996 + -1.0 4,963,094 + -0.2 4,963,094 + -0.3 4,963,094 + +0.3 4,963,094 + +0.7 4,963,095 - -0.0 4,963,095 - -0.3 4,963,095 - -0.1 4,963,095 - -0.5 4,963,095 - +0.2 4,963,095 - -0.3 4,963,188 - AMB_RS23050 0.18 -2.0 4,963,188 - AMB_RS23050 0.18 -0.3 4,963,390 + AMB_RS23050 0.38 -0.5 4,963,390 + AMB_RS23050 0.38 -0.5 4,963,418 + AMB_RS23050 0.41 +0.1 4,963,419 - AMB_RS23050 0.41 -0.7 4,963,425 + AMB_RS23050 0.42 -1.3 4,963,425 + AMB_RS23050 0.42 +0.1 4,963,425 + AMB_RS23050 0.42 +0.6 4,963,425 + AMB_RS23050 0.42 -0.4 4,963,425 + AMB_RS23050 0.42 -0.1 4,963,425 + AMB_RS23050 0.42 -0.0 4,963,426 - AMB_RS23050 0.42 -0.3 4,963,426 - AMB_RS23050 0.42 -1.1 4,963,426 - AMB_RS23050 0.42 -0.3 4,963,426 - AMB_RS23050 0.42 +1.0 4,963,426 - AMB_RS23050 0.42 -0.6 4,963,586 + AMB_RS23050 0.58 -0.3 4,963,587 - AMB_RS23050 0.58 -0.5 4,963,604 + AMB_RS23050 0.60 +0.9 4,963,604 + AMB_RS23050 0.60 -0.3 4,963,709 + AMB_RS23050 0.71 +1.2 4,963,709 + AMB_RS23050 0.71 +0.2 4,963,709 + AMB_RS23050 0.71 +0.2 4,963,710 - AMB_RS23050 0.71 +0.4 4,963,733 + AMB_RS23050 0.73 -2.0 4,963,733 + AMB_RS23050 0.73 -0.1 4,963,733 + AMB_RS23050 0.73 -0.2 4,963,835 + AMB_RS23050 0.84 -0.0 4,963,865 + AMB_RS23050 0.87 -0.5 4,963,865 + AMB_RS23050 0.87 +0.1 4,963,865 + AMB_RS23050 0.87 -1.2 4,963,865 + AMB_RS23050 0.87 -0.4 4,963,865 + AMB_RS23050 0.87 -0.1 4,963,866 - AMB_RS23050 0.87 +0.2 4,964,056 + AMB_RS23055 0.15 -1.6 4,964,182 + AMB_RS23055 0.43 -1.2 4,964,183 - AMB_RS23055 0.43 -1.0 4,964,296 + AMB_RS23055 0.68 +0.1 4,964,297 - AMB_RS23055 0.68 -0.5 4,964,451 + +0.0 4,964,524 + -0.6 4,964,524 + +0.8 4,964,524 + +0.0 4,964,524 + -0.0 4,964,525 - -0.6 4,964,526 + -0.3 4,964,526 + -0.1 4,964,526 + -2.8 4,964,716 + AMB_RS23060 0.13 +0.1 4,965,265 + AMB_RS23060 0.40 +0.6 4,965,266 - AMB_RS23060 0.40 -0.0 4,965,266 - AMB_RS23060 0.40 -0.2 4,965,415 - AMB_RS23060 0.48 -0.1
Or see this region's nucleotide sequence