Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS21250 and AMB_RS21255 are separated by 13 nucleotides AMB_RS21255 and AMB_RS21260 overlap by 4 nucleotides AMB_RS21260 and AMB_RS21265 overlap by 4 nucleotides AMB_RS21265 and AMB_RS21270 are separated by 69 nucleotides
AMB_RS21250: AMB_RS21250 - heme lyase CcmF/NrfE family subunit, at 4,613,844 to 4,615,823
_RS21250
AMB_RS21255: AMB_RS21255 - DsbE family thiol:disulfide interchange protein, at 4,615,837 to 4,616,379
_RS21255
AMB_RS21260: AMB_RS21260 - cytochrome c-type biogenesis protein CcmH, at 4,616,376 to 4,616,957
_RS21260
AMB_RS21265: AMB_RS21265 - c-type cytochrome biogenesis protein CcmI, at 4,616,954 to 4,617,787
_RS21265
AMB_RS21270: AMB_RS21270 - acetyl-CoA carboxylase carboxyl transferase subunit alpha, at 4,617,857 to 4,618,810
_RS21270
Position (kb) 4616
4617 Strain fitness (log2 ratio) -2
-1
0
1 at 4615.852 kb on + strand at 4615.852 kb on + strand at 4615.852 kb on + strand at 4617.279 kb on - strand, within AMB_RS21265 at 4617.279 kb on - strand, within AMB_RS21265 at 4617.279 kb on - strand, within AMB_RS21265 at 4617.452 kb on + strand, within AMB_RS21265 at 4617.491 kb on + strand, within AMB_RS21265 at 4617.650 kb on + strand, within AMB_RS21265 at 4617.650 kb on + strand, within AMB_RS21265 at 4617.650 kb on + strand, within AMB_RS21265 at 4617.650 kb on + strand, within AMB_RS21265 at 4617.651 kb on - strand, within AMB_RS21265 at 4617.651 kb on - strand, within AMB_RS21265 at 4617.651 kb on - strand, within AMB_RS21265 at 4617.651 kb on - strand, within AMB_RS21265 at 4617.843 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 4,615,852 + +0.0 4,615,852 + -2.1 4,615,852 + +0.7 4,617,279 - AMB_RS21265 0.39 +0.4 4,617,279 - AMB_RS21265 0.39 -1.6 4,617,279 - AMB_RS21265 0.39 -0.6 4,617,452 + AMB_RS21265 0.60 -2.4 4,617,491 + AMB_RS21265 0.64 -0.1 4,617,650 + AMB_RS21265 0.83 -0.5 4,617,650 + AMB_RS21265 0.83 -0.9 4,617,650 + AMB_RS21265 0.83 +1.4 4,617,650 + AMB_RS21265 0.83 -0.9 4,617,651 - AMB_RS21265 0.84 +1.5 4,617,651 - AMB_RS21265 0.84 -0.9 4,617,651 - AMB_RS21265 0.84 -1.4 4,617,651 - AMB_RS21265 0.84 -0.3 4,617,843 + -0.4
Or see this region's nucleotide sequence