Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS17075 and AMB_RS17080 are separated by 2 nucleotides AMB_RS17080 and AMB_RS17085 overlap by 4 nucleotides AMB_RS17085 and AMB_RS17090 are separated by 3 nucleotides
AMB_RS17075: AMB_RS17075 - FAD-dependent oxidoreductase, at 3,663,641 to 3,665,596
_RS17075
AMB_RS17080: AMB_RS17080 - Hdr-like menaquinol oxidoreductase cytochrome c subunit, at 3,665,599 to 3,665,973
_RS17080
AMB_RS17085: AMB_RS17085 - 4Fe-4S dicluster domain-containing protein, at 3,665,970 to 3,666,665
_RS17085
AMB_RS17090: AMB_RS17090 - molybdopterin oxidoreductase, at 3,666,669 to 3,667,880
_RS17090
Position (kb)
3665
3666
3667 Strain fitness (log2 ratio)
-1
0
1 at 3665.504 kb on + strand at 3665.520 kb on - strand at 3665.542 kb on + strand at 3665.543 kb on - strand at 3665.573 kb on - strand at 3665.594 kb on + strand at 3665.594 kb on + strand at 3665.595 kb on - strand at 3665.595 kb on - strand at 3665.624 kb on + strand at 3665.624 kb on + strand at 3665.896 kb on + strand, within AMB_RS17080 at 3665.943 kb on + strand at 3665.944 kb on - strand at 3666.186 kb on + strand, within AMB_RS17085 at 3666.186 kb on + strand, within AMB_RS17085 at 3666.186 kb on + strand, within AMB_RS17085 at 3666.186 kb on + strand, within AMB_RS17085 at 3666.187 kb on - strand, within AMB_RS17085 at 3666.187 kb on - strand, within AMB_RS17085 at 3666.187 kb on - strand, within AMB_RS17085 at 3666.187 kb on - strand, within AMB_RS17085 at 3666.360 kb on + strand, within AMB_RS17085 at 3666.381 kb on + strand, within AMB_RS17085 at 3666.381 kb on + strand, within AMB_RS17085 at 3666.381 kb on + strand, within AMB_RS17085 at 3666.382 kb on - strand, within AMB_RS17085 at 3666.652 kb on - strand at 3666.688 kb on - strand at 3666.688 kb on - strand at 3666.688 kb on - strand at 3666.705 kb on + strand at 3666.706 kb on - strand at 3666.924 kb on + strand, within AMB_RS17090 at 3666.924 kb on + strand, within AMB_RS17090 at 3666.924 kb on + strand, within AMB_RS17090 at 3666.924 kb on + strand, within AMB_RS17090 at 3666.925 kb on - strand, within AMB_RS17090 at 3667.080 kb on + strand, within AMB_RS17090 at 3667.080 kb on + strand, within AMB_RS17090 at 3667.081 kb on - strand, within AMB_RS17090 at 3667.110 kb on + strand, within AMB_RS17090 at 3667.111 kb on - strand, within AMB_RS17090 at 3667.150 kb on - strand, within AMB_RS17090 at 3667.150 kb on - strand, within AMB_RS17090 at 3667.242 kb on + strand, within AMB_RS17090 at 3667.242 kb on + strand, within AMB_RS17090 at 3667.243 kb on - strand, within AMB_RS17090 at 3667.291 kb on - strand, within AMB_RS17090 at 3667.414 kb on - strand, within AMB_RS17090 at 3667.446 kb on + strand, within AMB_RS17090 at 3667.447 kb on - strand, within AMB_RS17090 at 3667.564 kb on - strand, within AMB_RS17090
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 3,665,504 + +0.4 3,665,520 - +0.4 3,665,542 + -0.1 3,665,543 - -0.5 3,665,573 - -0.3 3,665,594 + -0.0 3,665,594 + -0.2 3,665,595 - -0.7 3,665,595 - -0.7 3,665,624 + -0.0 3,665,624 + -0.9 3,665,896 + AMB_RS17080 0.79 +0.3 3,665,943 + -0.2 3,665,944 - -1.3 3,666,186 + AMB_RS17085 0.31 -0.4 3,666,186 + AMB_RS17085 0.31 -0.9 3,666,186 + AMB_RS17085 0.31 +0.1 3,666,186 + AMB_RS17085 0.31 +1.0 3,666,187 - AMB_RS17085 0.31 -0.7 3,666,187 - AMB_RS17085 0.31 -0.4 3,666,187 - AMB_RS17085 0.31 +1.1 3,666,187 - AMB_RS17085 0.31 -0.3 3,666,360 + AMB_RS17085 0.56 -0.0 3,666,381 + AMB_RS17085 0.59 +0.4 3,666,381 + AMB_RS17085 0.59 -0.2 3,666,381 + AMB_RS17085 0.59 +0.3 3,666,382 - AMB_RS17085 0.59 -0.4 3,666,652 - -0.7 3,666,688 - -0.0 3,666,688 - -0.2 3,666,688 - -0.7 3,666,705 + +0.4 3,666,706 - -0.5 3,666,924 + AMB_RS17090 0.21 -0.3 3,666,924 + AMB_RS17090 0.21 -0.0 3,666,924 + AMB_RS17090 0.21 -0.1 3,666,924 + AMB_RS17090 0.21 -0.8 3,666,925 - AMB_RS17090 0.21 +0.7 3,667,080 + AMB_RS17090 0.34 +0.0 3,667,080 + AMB_RS17090 0.34 +1.0 3,667,081 - AMB_RS17090 0.34 +0.0 3,667,110 + AMB_RS17090 0.36 +0.5 3,667,111 - AMB_RS17090 0.36 +0.1 3,667,150 - AMB_RS17090 0.40 -0.6 3,667,150 - AMB_RS17090 0.40 +0.2 3,667,242 + AMB_RS17090 0.47 -0.4 3,667,242 + AMB_RS17090 0.47 -0.3 3,667,243 - AMB_RS17090 0.47 +0.7 3,667,291 - AMB_RS17090 0.51 +0.1 3,667,414 - AMB_RS17090 0.61 -0.3 3,667,446 + AMB_RS17090 0.64 +1.0 3,667,447 - AMB_RS17090 0.64 -0.2 3,667,564 - AMB_RS17090 0.74 +0.9
Or see this region's nucleotide sequence