Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14720 and AMB_RS14725 are separated by 3 nucleotides AMB_RS14725 and AMB_RS14730 are separated by 20 nucleotides AMB_RS14730 and AMB_RS14735 are separated by 3 nucleotides AMB_RS14735 and AMB_RS24560 are separated by 91 nucleotides
AMB_RS14720: AMB_RS14720 - NAD(P)/FAD-dependent oxidoreductase, at 3,119,022 to 3,120,272
_RS14720
AMB_RS14725: AMB_RS14725 - hypothetical protein, at 3,120,276 to 3,120,530
_RS14725
AMB_RS14730: AMB_RS14730 - hypothetical protein, at 3,120,551 to 3,120,796
_RS14730
AMB_RS14735: AMB_RS14735 - hypothetical protein, at 3,120,800 to 3,121,147
_RS14735
AMB_RS24560: AMB_RS24560 - hypothetical protein, at 3,121,239 to 3,122,357
_RS24560
Position (kb)
3120
3121 Strain fitness (log2 ratio)
-2
-1
0
1 at 3119.319 kb on + strand, within AMB_RS14720 at 3119.808 kb on + strand, within AMB_RS14720 at 3119.808 kb on + strand, within AMB_RS14720 at 3119.808 kb on + strand, within AMB_RS14720 at 3119.808 kb on + strand, within AMB_RS14720 at 3119.808 kb on + strand, within AMB_RS14720 at 3119.809 kb on - strand, within AMB_RS14720 at 3119.809 kb on - strand, within AMB_RS14720 at 3119.809 kb on - strand, within AMB_RS14720 at 3119.873 kb on + strand, within AMB_RS14720 at 3120.088 kb on - strand, within AMB_RS14720 at 3120.246 kb on + strand at 3120.246 kb on + strand at 3120.246 kb on + strand at 3120.247 kb on - strand at 3120.247 kb on - strand at 3120.271 kb on - strand at 3120.427 kb on + strand, within AMB_RS14725 at 3120.427 kb on + strand, within AMB_RS14725 at 3120.427 kb on + strand, within AMB_RS14725 at 3120.427 kb on + strand, within AMB_RS14725 at 3120.427 kb on + strand, within AMB_RS14725 at 3120.428 kb on - strand, within AMB_RS14725 at 3120.428 kb on - strand, within AMB_RS14725 at 3120.428 kb on - strand, within AMB_RS14725 at 3120.428 kb on - strand, within AMB_RS14725 at 3120.605 kb on + strand, within AMB_RS14730 at 3120.605 kb on + strand, within AMB_RS14730 at 3120.605 kb on + strand, within AMB_RS14730 at 3120.606 kb on - strand, within AMB_RS14730 at 3120.606 kb on - strand, within AMB_RS14730 at 3120.606 kb on - strand, within AMB_RS14730 at 3120.606 kb on - strand, within AMB_RS14730 at 3120.606 kb on - strand, within AMB_RS14730 at 3120.606 kb on - strand, within AMB_RS14730 at 3120.606 kb on - strand, within AMB_RS14730 at 3120.606 kb on - strand, within AMB_RS14730 at 3120.794 kb on + strand at 3120.795 kb on - strand at 3120.831 kb on + strand at 3120.832 kb on - strand at 3121.039 kb on - strand, within AMB_RS14735 at 3121.039 kb on - strand, within AMB_RS14735 at 3121.191 kb on - strand at 3121.376 kb on + strand, within AMB_RS24560 at 3121.377 kb on - strand, within AMB_RS24560 at 3121.451 kb on - strand, within AMB_RS24560
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 3,119,319 + AMB_RS14720 0.24 +0.0 3,119,808 + AMB_RS14720 0.63 +0.2 3,119,808 + AMB_RS14720 0.63 -0.1 3,119,808 + AMB_RS14720 0.63 -0.3 3,119,808 + AMB_RS14720 0.63 +0.4 3,119,808 + AMB_RS14720 0.63 -2.4 3,119,809 - AMB_RS14720 0.63 -1.6 3,119,809 - AMB_RS14720 0.63 -0.1 3,119,809 - AMB_RS14720 0.63 +0.4 3,119,873 + AMB_RS14720 0.68 -0.4 3,120,088 - AMB_RS14720 0.85 +0.2 3,120,246 + -1.5 3,120,246 + -0.5 3,120,246 + -0.1 3,120,247 - -0.2 3,120,247 - -0.8 3,120,271 - +0.8 3,120,427 + AMB_RS14725 0.59 -0.1 3,120,427 + AMB_RS14725 0.59 +0.2 3,120,427 + AMB_RS14725 0.59 +0.4 3,120,427 + AMB_RS14725 0.59 -0.6 3,120,427 + AMB_RS14725 0.59 -0.1 3,120,428 - AMB_RS14725 0.60 -0.1 3,120,428 - AMB_RS14725 0.60 +0.6 3,120,428 - AMB_RS14725 0.60 -0.6 3,120,428 - AMB_RS14725 0.60 -0.0 3,120,605 + AMB_RS14730 0.22 +0.0 3,120,605 + AMB_RS14730 0.22 -0.1 3,120,605 + AMB_RS14730 0.22 -1.1 3,120,606 - AMB_RS14730 0.22 +0.0 3,120,606 - AMB_RS14730 0.22 +0.2 3,120,606 - AMB_RS14730 0.22 +0.0 3,120,606 - AMB_RS14730 0.22 -0.2 3,120,606 - AMB_RS14730 0.22 -0.1 3,120,606 - AMB_RS14730 0.22 +1.3 3,120,606 - AMB_RS14730 0.22 -0.3 3,120,606 - AMB_RS14730 0.22 -0.1 3,120,794 + +0.2 3,120,795 - -0.1 3,120,831 + -1.6 3,120,832 - -0.2 3,121,039 - AMB_RS14735 0.69 +0.2 3,121,039 - AMB_RS14735 0.69 -0.0 3,121,191 - -0.9 3,121,376 + AMB_RS24560 0.12 -0.3 3,121,377 - AMB_RS24560 0.12 -0.0 3,121,451 - AMB_RS24560 0.19 -0.2
Or see this region's nucleotide sequence