Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11400 and AMB_RS11405 are separated by 143 nucleotides AMB_RS11405 and AMB_RS11410 overlap by 4 nucleotides AMB_RS11410 and AMB_RS11415 are separated by 95 nucleotides AMB_RS11415 and AMB_RS11420 are separated by 93 nucleotides
AMB_RS11400: AMB_RS11400 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 2,457,018 to 2,459,183
_RS11400
AMB_RS11405: AMB_RS11405 - pyridoxine 5'-phosphate synthase, at 2,459,327 to 2,460,058
_RS11405
AMB_RS11410: AMB_RS11410 - nuclear transport factor 2 family protein, at 2,460,055 to 2,460,480
_RS11410
AMB_RS11415: AMB_RS11415 - holo-ACP synthase, at 2,460,576 to 2,460,971
_RS11415
AMB_RS11420: AMB_RS11420 - signal peptidase I, at 2,461,065 to 2,461,808
_RS11420
Position (kb)
2460
2461 Strain fitness (log2 ratio)
-2
-1
0
1 at 2459.181 kb on + strand at 2459.182 kb on - strand at 2459.189 kb on - strand at 2459.208 kb on + strand at 2459.208 kb on + strand at 2459.261 kb on + strand at 2460.056 kb on + strand at 2460.056 kb on + strand at 2460.057 kb on - strand at 2460.057 kb on - strand at 2460.057 kb on - strand at 2460.057 kb on - strand at 2460.239 kb on + strand, within AMB_RS11410 at 2460.239 kb on + strand, within AMB_RS11410 at 2460.240 kb on - strand, within AMB_RS11410 at 2460.293 kb on + strand, within AMB_RS11410 at 2460.294 kb on - strand, within AMB_RS11410 at 2460.294 kb on - strand, within AMB_RS11410 at 2460.294 kb on - strand, within AMB_RS11410 at 2460.294 kb on - strand, within AMB_RS11410 at 2460.294 kb on - strand, within AMB_RS11410 at 2460.294 kb on - strand, within AMB_RS11410 at 2460.294 kb on - strand, within AMB_RS11410 at 2460.294 kb on - strand, within AMB_RS11410 at 2460.336 kb on - strand, within AMB_RS11410 at 2460.452 kb on + strand at 2460.541 kb on - strand at 2460.541 kb on - strand at 2460.541 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 2,459,181 + -0.1 2,459,182 - -2.0 2,459,189 - -1.4 2,459,208 + -0.5 2,459,208 + +0.1 2,459,261 + -1.4 2,460,056 + +0.1 2,460,056 + +0.0 2,460,057 - +0.0 2,460,057 - -0.3 2,460,057 - +0.5 2,460,057 - -0.1 2,460,239 + AMB_RS11410 0.43 -0.0 2,460,239 + AMB_RS11410 0.43 -0.8 2,460,240 - AMB_RS11410 0.43 +0.0 2,460,293 + AMB_RS11410 0.56 +0.3 2,460,294 - AMB_RS11410 0.56 +0.0 2,460,294 - AMB_RS11410 0.56 -0.1 2,460,294 - AMB_RS11410 0.56 +0.3 2,460,294 - AMB_RS11410 0.56 -0.2 2,460,294 - AMB_RS11410 0.56 -0.4 2,460,294 - AMB_RS11410 0.56 -0.6 2,460,294 - AMB_RS11410 0.56 -0.2 2,460,294 - AMB_RS11410 0.56 +1.0 2,460,336 - AMB_RS11410 0.66 -0.6 2,460,452 + +0.8 2,460,541 - -1.4 2,460,541 - +0.0 2,460,541 - +0.1
Or see this region's nucleotide sequence