Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10240 and AMB_RS10245 overlap by 4 nucleotides AMB_RS10245 and AMB_RS10250 are separated by 40 nucleotides AMB_RS10250 and AMB_RS10255 are separated by 225 nucleotides
AMB_RS10240: AMB_RS10240 - branched-chain amino acid ABC transporter permease, at 2,205,501 to 2,206,490
_RS10240
AMB_RS10245: AMB_RS10245 - branched-chain amino acid ABC transporter permease, at 2,206,487 to 2,207,356
_RS10245
AMB_RS10250: AMB_RS10250 - branched-chain amino acid ABC transporter, at 2,207,397 to 2,208,650
_RS10250
AMB_RS10255: AMB_RS10255 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 2,208,876 to 2,209,853
_RS10255
Position (kb)
2207
2208
2209 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2206.402 kb on + strand at 2206.402 kb on + strand at 2206.402 kb on + strand at 2206.403 kb on - strand at 2206.689 kb on + strand, within AMB_RS10245 at 2206.785 kb on + strand, within AMB_RS10245 at 2206.785 kb on + strand, within AMB_RS10245 at 2206.869 kb on + strand, within AMB_RS10245 at 2206.962 kb on + strand, within AMB_RS10245 at 2206.963 kb on - strand, within AMB_RS10245 at 2206.963 kb on - strand, within AMB_RS10245 at 2206.963 kb on - strand, within AMB_RS10245 at 2207.121 kb on + strand, within AMB_RS10245 at 2207.208 kb on + strand, within AMB_RS10245 at 2207.208 kb on + strand, within AMB_RS10245 at 2207.208 kb on + strand, within AMB_RS10245 at 2207.208 kb on + strand, within AMB_RS10245 at 2207.208 kb on + strand, within AMB_RS10245 at 2207.208 kb on + strand, within AMB_RS10245 at 2207.209 kb on - strand, within AMB_RS10245 at 2207.209 kb on - strand, within AMB_RS10245 at 2207.209 kb on - strand, within AMB_RS10245 at 2207.209 kb on - strand, within AMB_RS10245 at 2207.209 kb on - strand, within AMB_RS10245 at 2207.298 kb on + strand at 2207.779 kb on + strand, within AMB_RS10250 at 2207.779 kb on + strand, within AMB_RS10250 at 2207.780 kb on - strand, within AMB_RS10250 at 2207.780 kb on - strand, within AMB_RS10250 at 2207.789 kb on - strand, within AMB_RS10250 at 2207.821 kb on + strand, within AMB_RS10250 at 2207.821 kb on + strand, within AMB_RS10250 at 2207.821 kb on + strand, within AMB_RS10250 at 2207.821 kb on + strand, within AMB_RS10250 at 2207.822 kb on - strand, within AMB_RS10250 at 2207.822 kb on - strand, within AMB_RS10250 at 2207.993 kb on - strand, within AMB_RS10250 at 2207.993 kb on - strand, within AMB_RS10250 at 2208.086 kb on + strand, within AMB_RS10250 at 2208.086 kb on + strand, within AMB_RS10250 at 2208.086 kb on + strand, within AMB_RS10250 at 2208.087 kb on - strand, within AMB_RS10250 at 2208.087 kb on - strand, within AMB_RS10250 at 2208.087 kb on - strand, within AMB_RS10250 at 2208.142 kb on + strand, within AMB_RS10250 at 2208.142 kb on + strand, within AMB_RS10250 at 2208.142 kb on + strand, within AMB_RS10250 at 2208.142 kb on + strand, within AMB_RS10250 at 2208.143 kb on - strand, within AMB_RS10250 at 2208.143 kb on - strand, within AMB_RS10250 at 2208.199 kb on + strand, within AMB_RS10250 at 2208.199 kb on + strand, within AMB_RS10250 at 2208.199 kb on + strand, within AMB_RS10250 at 2208.199 kb on + strand, within AMB_RS10250 at 2208.200 kb on - strand, within AMB_RS10250 at 2208.200 kb on - strand, within AMB_RS10250 at 2208.200 kb on - strand, within AMB_RS10250 at 2208.200 kb on - strand, within AMB_RS10250 at 2208.200 kb on - strand, within AMB_RS10250 at 2208.200 kb on - strand, within AMB_RS10250 at 2208.412 kb on + strand, within AMB_RS10250 at 2208.413 kb on - strand, within AMB_RS10250 at 2208.801 kb on + strand at 2208.843 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 2,206,402 + +0.6 2,206,402 + -1.0 2,206,402 + +0.0 2,206,403 - -1.7 2,206,689 + AMB_RS10245 0.23 -0.0 2,206,785 + AMB_RS10245 0.34 +0.2 2,206,785 + AMB_RS10245 0.34 -1.7 2,206,869 + AMB_RS10245 0.44 -0.1 2,206,962 + AMB_RS10245 0.55 -1.8 2,206,963 - AMB_RS10245 0.55 +0.1 2,206,963 - AMB_RS10245 0.55 -0.5 2,206,963 - AMB_RS10245 0.55 +0.1 2,207,121 + AMB_RS10245 0.73 +0.2 2,207,208 + AMB_RS10245 0.83 +1.3 2,207,208 + AMB_RS10245 0.83 -1.1 2,207,208 + AMB_RS10245 0.83 -0.0 2,207,208 + AMB_RS10245 0.83 +0.1 2,207,208 + AMB_RS10245 0.83 +0.0 2,207,208 + AMB_RS10245 0.83 -0.0 2,207,209 - AMB_RS10245 0.83 -0.3 2,207,209 - AMB_RS10245 0.83 +1.3 2,207,209 - AMB_RS10245 0.83 -0.3 2,207,209 - AMB_RS10245 0.83 +0.1 2,207,209 - AMB_RS10245 0.83 -0.3 2,207,298 + +0.0 2,207,779 + AMB_RS10250 0.30 +0.5 2,207,779 + AMB_RS10250 0.30 +0.4 2,207,780 - AMB_RS10250 0.31 -0.3 2,207,780 - AMB_RS10250 0.31 -0.4 2,207,789 - AMB_RS10250 0.31 -0.0 2,207,821 + AMB_RS10250 0.34 -0.1 2,207,821 + AMB_RS10250 0.34 +0.2 2,207,821 + AMB_RS10250 0.34 +0.9 2,207,821 + AMB_RS10250 0.34 -0.0 2,207,822 - AMB_RS10250 0.34 -0.1 2,207,822 - AMB_RS10250 0.34 -0.4 2,207,993 - AMB_RS10250 0.48 +0.4 2,207,993 - AMB_RS10250 0.48 -0.7 2,208,086 + AMB_RS10250 0.55 -0.1 2,208,086 + AMB_RS10250 0.55 -0.1 2,208,086 + AMB_RS10250 0.55 +0.5 2,208,087 - AMB_RS10250 0.55 +0.5 2,208,087 - AMB_RS10250 0.55 -0.5 2,208,087 - AMB_RS10250 0.55 -0.0 2,208,142 + AMB_RS10250 0.59 -0.3 2,208,142 + AMB_RS10250 0.59 -1.0 2,208,142 + AMB_RS10250 0.59 -0.8 2,208,142 + AMB_RS10250 0.59 -0.6 2,208,143 - AMB_RS10250 0.59 -0.6 2,208,143 - AMB_RS10250 0.59 -2.0 2,208,199 + AMB_RS10250 0.64 +0.2 2,208,199 + AMB_RS10250 0.64 +0.4 2,208,199 + AMB_RS10250 0.64 +0.4 2,208,199 + AMB_RS10250 0.64 -0.0 2,208,200 - AMB_RS10250 0.64 -3.0 2,208,200 - AMB_RS10250 0.64 +0.4 2,208,200 - AMB_RS10250 0.64 +0.1 2,208,200 - AMB_RS10250 0.64 +0.7 2,208,200 - AMB_RS10250 0.64 -0.7 2,208,200 - AMB_RS10250 0.64 +0.1 2,208,412 + AMB_RS10250 0.81 -1.1 2,208,413 - AMB_RS10250 0.81 -0.5 2,208,801 + +0.3 2,208,843 + -0.8
Or see this region's nucleotide sequence