Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS08035 and AMB_RS08040 are separated by 123 nucleotides AMB_RS08040 and AMB_RS08045 are separated by 185 nucleotides AMB_RS08045 and AMB_RS08050 are separated by 155 nucleotides AMB_RS08050 and AMB_RS08055 are separated by 164 nucleotides
AMB_RS08035: AMB_RS08035 - CBS domain-containing protein, at 1,721,318 to 1,721,755
_RS08035
AMB_RS08040: AMB_RS08040 - cold-shock protein, at 1,721,879 to 1,722,088
_RS08040
AMB_RS08045: AMB_RS08045 - hypothetical protein, at 1,722,274 to 1,722,780
_RS08045
AMB_RS08050: AMB_RS08050 - rhodanese-like domain-containing protein, at 1,722,936 to 1,723,271
_RS08050
AMB_RS08055: AMB_RS08055 - hypothetical protein, at 1,723,436 to 1,723,891
_RS08055
Position (kb)
1722
1723 Strain fitness (log2 ratio)
-1
0
1 at 1721.292 kb on - strand at 1721.443 kb on + strand, within AMB_RS08035 at 1721.707 kb on + strand, within AMB_RS08035 at 1721.790 kb on + strand at 1721.791 kb on - strand at 1721.791 kb on - strand at 1721.791 kb on - strand at 1721.791 kb on - strand at 1721.791 kb on - strand at 1721.880 kb on + strand at 1721.881 kb on - strand at 1721.911 kb on + strand, within AMB_RS08040 at 1721.911 kb on + strand, within AMB_RS08040 at 1721.911 kb on + strand, within AMB_RS08040 at 1721.912 kb on - strand, within AMB_RS08040 at 1721.912 kb on - strand, within AMB_RS08040 at 1721.912 kb on - strand, within AMB_RS08040 at 1722.038 kb on - strand, within AMB_RS08040 at 1722.056 kb on - strand, within AMB_RS08040 at 1722.070 kb on + strand at 1722.070 kb on + strand at 1722.071 kb on - strand at 1722.071 kb on - strand at 1722.102 kb on + strand at 1722.106 kb on + strand at 1722.106 kb on + strand at 1722.106 kb on + strand at 1722.107 kb on - strand at 1722.109 kb on + strand at 1722.109 kb on + strand at 1722.110 kb on - strand at 1722.110 kb on - strand at 1722.112 kb on + strand at 1722.205 kb on + strand at 1722.246 kb on - strand at 1722.246 kb on - strand at 1722.275 kb on + strand at 1722.276 kb on - strand at 1722.296 kb on + strand at 1722.581 kb on + strand, within AMB_RS08045 at 1722.581 kb on + strand, within AMB_RS08045 at 1722.581 kb on + strand, within AMB_RS08045 at 1722.581 kb on + strand, within AMB_RS08045 at 1722.582 kb on - strand, within AMB_RS08045 at 1722.582 kb on - strand, within AMB_RS08045 at 1722.584 kb on + strand, within AMB_RS08045 at 1722.584 kb on + strand, within AMB_RS08045 at 1722.585 kb on - strand, within AMB_RS08045 at 1722.585 kb on - strand, within AMB_RS08045 at 1722.771 kb on + strand at 1722.828 kb on - strand at 1722.916 kb on - strand at 1723.056 kb on - strand, within AMB_RS08050 at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.290 kb on - strand at 1723.290 kb on - strand at 1723.358 kb on - strand at 1723.358 kb on - strand at 1723.358 kb on - strand at 1723.362 kb on - strand at 1723.362 kb on - strand at 1723.376 kb on + strand at 1723.376 kb on + strand at 1723.377 kb on - strand at 1723.377 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 1,721,292 - -0.5 1,721,443 + AMB_RS08035 0.29 -0.2 1,721,707 + AMB_RS08035 0.89 +0.7 1,721,790 + +0.4 1,721,791 - -0.4 1,721,791 - +0.2 1,721,791 - +0.1 1,721,791 - +0.1 1,721,791 - -0.6 1,721,880 + -0.4 1,721,881 - -0.1 1,721,911 + AMB_RS08040 0.15 +0.1 1,721,911 + AMB_RS08040 0.15 -0.2 1,721,911 + AMB_RS08040 0.15 -0.3 1,721,912 - AMB_RS08040 0.16 -0.0 1,721,912 - AMB_RS08040 0.16 -0.7 1,721,912 - AMB_RS08040 0.16 +0.2 1,722,038 - AMB_RS08040 0.76 +0.3 1,722,056 - AMB_RS08040 0.84 -0.8 1,722,070 + -1.5 1,722,070 + -0.3 1,722,071 - +0.1 1,722,071 - -0.0 1,722,102 + -0.6 1,722,106 + -0.6 1,722,106 + -0.6 1,722,106 + -0.7 1,722,107 - +0.3 1,722,109 + -0.8 1,722,109 + -0.2 1,722,110 - -0.6 1,722,110 - -0.4 1,722,112 + +0.4 1,722,205 + -0.0 1,722,246 - +0.3 1,722,246 - -0.0 1,722,275 + -0.9 1,722,276 - +0.9 1,722,296 + -0.6 1,722,581 + AMB_RS08045 0.61 -1.5 1,722,581 + AMB_RS08045 0.61 -0.9 1,722,581 + AMB_RS08045 0.61 +0.2 1,722,581 + AMB_RS08045 0.61 -0.4 1,722,582 - AMB_RS08045 0.61 -0.2 1,722,582 - AMB_RS08045 0.61 +0.7 1,722,584 + AMB_RS08045 0.61 -1.3 1,722,584 + AMB_RS08045 0.61 -0.6 1,722,585 - AMB_RS08045 0.61 -0.4 1,722,585 - AMB_RS08045 0.61 -0.0 1,722,771 + -0.6 1,722,828 - -0.4 1,722,916 - -0.4 1,723,056 - AMB_RS08050 0.36 -0.8 1,723,289 + -0.3 1,723,289 + +0.4 1,723,289 + -0.4 1,723,289 + +1.7 1,723,289 + +0.5 1,723,289 + +1.2 1,723,289 + -0.5 1,723,289 + -0.3 1,723,290 - -0.0 1,723,290 - -0.4 1,723,358 - -0.0 1,723,358 - -0.2 1,723,358 - -0.2 1,723,362 - +1.0 1,723,362 - -0.4 1,723,376 + -0.1 1,723,376 + -1.2 1,723,377 - -0.3 1,723,377 - +0.1
Or see this region's nucleotide sequence