Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07865 and AMB_RS07870 are separated by 325 nucleotides AMB_RS07870 and AMB_RS07875 overlap by 4 nucleotides AMB_RS07875 and AMB_RS07880 are separated by 11 nucleotides AMB_RS07880 and AMB_RS07885 are separated by 81 nucleotides
AMB_RS07865: AMB_RS07865 - ATP-dependent chaperone ClpB, at 1,689,038 to 1,691,629
_RS07865
AMB_RS07870: AMB_RS07870 - nitrogenase stabilizing/protective protein nifW, at 1,691,955 to 1,692,263
_RS07870
AMB_RS07875: AMB_RS07875 - homocitrate synthase, at 1,692,260 to 1,693,405
_RS07875
AMB_RS07880: AMB_RS07880 - iron-sulfur cluster assembly accessory protein, at 1,693,417 to 1,693,755
_RS07880
AMB_RS07885: AMB_RS07885 - nitrogen fixation protein NifQ, at 1,693,837 to 1,694,454
_RS07885
Position (kb)
1692
1693
1694 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1691.456 kb on + strand at 1691.456 kb on + strand at 1691.456 kb on + strand at 1691.457 kb on - strand at 1691.457 kb on - strand at 1691.468 kb on + strand at 1691.469 kb on - strand at 1691.713 kb on - strand at 1691.713 kb on - strand at 1691.713 kb on - strand at 1691.933 kb on + strand at 1691.933 kb on + strand at 1691.934 kb on - strand at 1691.934 kb on - strand at 1692.061 kb on + strand, within AMB_RS07870 at 1692.061 kb on + strand, within AMB_RS07870 at 1692.062 kb on - strand, within AMB_RS07870 at 1692.062 kb on - strand, within AMB_RS07870 at 1692.492 kb on - strand, within AMB_RS07875 at 1692.492 kb on - strand, within AMB_RS07875 at 1692.501 kb on + strand, within AMB_RS07875 at 1692.501 kb on + strand, within AMB_RS07875 at 1692.501 kb on + strand, within AMB_RS07875 at 1692.501 kb on + strand, within AMB_RS07875 at 1692.502 kb on - strand, within AMB_RS07875 at 1692.502 kb on - strand, within AMB_RS07875 at 1692.502 kb on - strand, within AMB_RS07875 at 1692.827 kb on + strand, within AMB_RS07875 at 1692.828 kb on - strand, within AMB_RS07875 at 1692.828 kb on - strand, within AMB_RS07875 at 1693.372 kb on + strand at 1693.419 kb on - strand at 1693.532 kb on + strand, within AMB_RS07880 at 1693.532 kb on + strand, within AMB_RS07880 at 1693.532 kb on + strand, within AMB_RS07880 at 1693.533 kb on - strand, within AMB_RS07880 at 1693.533 kb on - strand, within AMB_RS07880 at 1693.533 kb on - strand, within AMB_RS07880 at 1693.533 kb on - strand, within AMB_RS07880 at 1693.533 kb on - strand, within AMB_RS07880 at 1693.649 kb on + strand, within AMB_RS07880 at 1693.650 kb on - strand, within AMB_RS07880 at 1693.650 kb on - strand, within AMB_RS07880 at 1693.650 kb on - strand, within AMB_RS07880 at 1693.650 kb on - strand, within AMB_RS07880 at 1693.697 kb on + strand, within AMB_RS07880 at 1693.697 kb on + strand, within AMB_RS07880 at 1693.698 kb on - strand, within AMB_RS07880 at 1693.698 kb on - strand, within AMB_RS07880 at 1693.698 kb on - strand, within AMB_RS07880 at 1693.992 kb on - strand, within AMB_RS07885
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 1,691,456 + -0.5 1,691,456 + -2.1 1,691,456 + -0.1 1,691,457 - -1.0 1,691,457 - -2.6 1,691,468 + -1.2 1,691,469 - -0.6 1,691,713 - -0.5 1,691,713 - -2.6 1,691,713 - -0.4 1,691,933 + -0.2 1,691,933 + -3.4 1,691,934 - -1.0 1,691,934 - +0.1 1,692,061 + AMB_RS07870 0.34 -0.0 1,692,061 + AMB_RS07870 0.34 -0.0 1,692,062 - AMB_RS07870 0.35 -0.7 1,692,062 - AMB_RS07870 0.35 -0.6 1,692,492 - AMB_RS07875 0.20 -1.0 1,692,492 - AMB_RS07875 0.20 -0.8 1,692,501 + AMB_RS07875 0.21 -0.3 1,692,501 + AMB_RS07875 0.21 -1.1 1,692,501 + AMB_RS07875 0.21 -0.2 1,692,501 + AMB_RS07875 0.21 -0.0 1,692,502 - AMB_RS07875 0.21 -1.4 1,692,502 - AMB_RS07875 0.21 +1.1 1,692,502 - AMB_RS07875 0.21 -1.5 1,692,827 + AMB_RS07875 0.49 -0.8 1,692,828 - AMB_RS07875 0.50 -0.0 1,692,828 - AMB_RS07875 0.50 -0.0 1,693,372 + -0.6 1,693,419 - +0.2 1,693,532 + AMB_RS07880 0.34 -0.0 1,693,532 + AMB_RS07880 0.34 -0.5 1,693,532 + AMB_RS07880 0.34 +0.3 1,693,533 - AMB_RS07880 0.34 -0.3 1,693,533 - AMB_RS07880 0.34 +1.0 1,693,533 - AMB_RS07880 0.34 -1.2 1,693,533 - AMB_RS07880 0.34 +0.3 1,693,533 - AMB_RS07880 0.34 +0.1 1,693,649 + AMB_RS07880 0.68 -1.3 1,693,650 - AMB_RS07880 0.69 -0.7 1,693,650 - AMB_RS07880 0.69 +0.4 1,693,650 - AMB_RS07880 0.69 +1.3 1,693,650 - AMB_RS07880 0.69 +0.5 1,693,697 + AMB_RS07880 0.83 -0.6 1,693,697 + AMB_RS07880 0.83 +0.2 1,693,698 - AMB_RS07880 0.83 -0.0 1,693,698 - AMB_RS07880 0.83 -0.5 1,693,698 - AMB_RS07880 0.83 -0.0 1,693,992 - AMB_RS07885 0.25 +0.3
Or see this region's nucleotide sequence