Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07555 and AMB_RS07560 are separated by 6 nucleotides AMB_RS07560 and AMB_RS07565 are separated by 5 nucleotides AMB_RS07565 and AMB_RS07570 overlap by 1 nucleotides AMB_RS07570 and AMB_RS07575 overlap by 4 nucleotides AMB_RS07575 and AMB_RS23300 overlap by 4 nucleotides
AMB_RS07555: AMB_RS07555 - DUF1566 domain-containing protein, at 1,635,284 to 1,635,856
_RS07555
AMB_RS07560: AMB_RS07560 - hypothetical protein, at 1,635,863 to 1,636,198
_RS07560
AMB_RS07565: AMB_RS07565 - single-stranded DNA-binding protein, at 1,636,204 to 1,636,785
_RS07565
AMB_RS07570: AMB_RS07570 - hypothetical protein, at 1,636,785 to 1,637,390
_RS07570
AMB_RS07575: AMB_RS07575 - hypothetical protein, at 1,637,387 to 1,637,692
_RS07575
AMB_RS23300: AMB_RS23300 - transcriptional regulator, at 1,637,689 to 1,639,923
_RS23300
Position (kb)
1636
1637
1638 Strain fitness (log2 ratio)
-2
-1
0
1 at 1635.839 kb on - strand at 1635.844 kb on + strand at 1635.844 kb on + strand at 1635.844 kb on + strand at 1635.844 kb on + strand at 1635.845 kb on - strand at 1635.845 kb on - strand at 1635.845 kb on - strand at 1635.864 kb on + strand at 1636.370 kb on + strand, within AMB_RS07565 at 1636.370 kb on + strand, within AMB_RS07565 at 1636.493 kb on + strand, within AMB_RS07565 at 1636.493 kb on + strand, within AMB_RS07565 at 1636.550 kb on + strand, within AMB_RS07565 at 1636.551 kb on - strand, within AMB_RS07565 at 1636.786 kb on + strand at 1636.787 kb on - strand at 1637.008 kb on + strand, within AMB_RS07570 at 1637.008 kb on + strand, within AMB_RS07570 at 1637.008 kb on + strand, within AMB_RS07570 at 1637.643 kb on + strand, within AMB_RS07575 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.069 kb on - strand, within AMB_RS23300 at 1638.069 kb on - strand, within AMB_RS23300 at 1638.069 kb on - strand, within AMB_RS23300 at 1638.114 kb on + strand, within AMB_RS23300 at 1638.115 kb on - strand, within AMB_RS23300 at 1638.299 kb on + strand, within AMB_RS23300 at 1638.300 kb on - strand, within AMB_RS23300 at 1638.300 kb on - strand, within AMB_RS23300 at 1638.300 kb on - strand, within AMB_RS23300
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 1,635,839 - +0.3 1,635,844 + -1.2 1,635,844 + -0.4 1,635,844 + -0.1 1,635,844 + -0.1 1,635,845 - -0.9 1,635,845 - +0.3 1,635,845 - -1.2 1,635,864 + +0.0 1,636,370 + AMB_RS07565 0.29 +0.1 1,636,370 + AMB_RS07565 0.29 -0.1 1,636,493 + AMB_RS07565 0.50 -0.2 1,636,493 + AMB_RS07565 0.50 +0.2 1,636,550 + AMB_RS07565 0.59 -0.1 1,636,551 - AMB_RS07565 0.60 -0.1 1,636,786 + +0.2 1,636,787 - -2.6 1,637,008 + AMB_RS07570 0.37 +0.9 1,637,008 + AMB_RS07570 0.37 -1.1 1,637,008 + AMB_RS07570 0.37 -1.0 1,637,643 + AMB_RS07575 0.84 +1.3 1,638,068 + AMB_RS23300 0.17 -0.2 1,638,068 + AMB_RS23300 0.17 +0.3 1,638,068 + AMB_RS23300 0.17 -0.1 1,638,068 + AMB_RS23300 0.17 +0.4 1,638,068 + AMB_RS23300 0.17 -0.1 1,638,068 + AMB_RS23300 0.17 -0.6 1,638,068 + AMB_RS23300 0.17 -1.6 1,638,068 + AMB_RS23300 0.17 -0.1 1,638,068 + AMB_RS23300 0.17 +0.1 1,638,068 + AMB_RS23300 0.17 +0.5 1,638,068 + AMB_RS23300 0.17 +0.5 1,638,068 + AMB_RS23300 0.17 +0.2 1,638,069 - AMB_RS23300 0.17 -0.7 1,638,069 - AMB_RS23300 0.17 -0.4 1,638,069 - AMB_RS23300 0.17 -0.6 1,638,114 + AMB_RS23300 0.19 -0.5 1,638,115 - AMB_RS23300 0.19 -0.2 1,638,299 + AMB_RS23300 0.27 +0.2 1,638,300 - AMB_RS23300 0.27 +0.8 1,638,300 - AMB_RS23300 0.27 +0.7 1,638,300 - AMB_RS23300 0.27 +0.6
Or see this region's nucleotide sequence