Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04735 and AMB_RS04740 are separated by 116 nucleotides AMB_RS04740 and AMB_RS23160 are separated by 76 nucleotides
AMB_RS04735: AMB_RS04735 - diguanylate cyclase, at 988,098 to 989,627
_RS04735
AMB_RS04740: AMB_RS04740 - hypothetical protein, at 989,744 to 990,139
_RS04740
AMB_RS23160: AMB_RS23160 - PAS domain S-box protein, at 990,216 to 992,045
_RS23160
Position (kb) 989
990
991 Strain fitness (log2 ratio) -2
-1
0
1 at 988.771 kb on - strand, within AMB_RS04735 at 988.771 kb on - strand, within AMB_RS04735 at 988.814 kb on + strand, within AMB_RS04735 at 988.814 kb on + strand, within AMB_RS04735 at 988.815 kb on - strand, within AMB_RS04735 at 988.815 kb on - strand, within AMB_RS04735 at 988.878 kb on - strand, within AMB_RS04735 at 988.946 kb on + strand, within AMB_RS04735 at 988.947 kb on - strand, within AMB_RS04735 at 988.947 kb on - strand, within AMB_RS04735 at 988.997 kb on + strand, within AMB_RS04735 at 988.998 kb on - strand, within AMB_RS04735 at 988.998 kb on - strand, within AMB_RS04735 at 989.003 kb on + strand, within AMB_RS04735 at 989.003 kb on + strand, within AMB_RS04735 at 989.004 kb on - strand, within AMB_RS04735 at 989.118 kb on + strand, within AMB_RS04735 at 989.118 kb on + strand, within AMB_RS04735 at 989.118 kb on + strand, within AMB_RS04735 at 989.119 kb on - strand, within AMB_RS04735 at 989.150 kb on + strand, within AMB_RS04735 at 989.150 kb on + strand, within AMB_RS04735 at 989.150 kb on + strand, within AMB_RS04735 at 989.150 kb on + strand, within AMB_RS04735 at 989.150 kb on + strand, within AMB_RS04735 at 989.151 kb on - strand, within AMB_RS04735 at 989.151 kb on - strand, within AMB_RS04735 at 989.151 kb on - strand, within AMB_RS04735 at 989.153 kb on + strand, within AMB_RS04735 at 989.153 kb on + strand, within AMB_RS04735 at 989.208 kb on + strand, within AMB_RS04735 at 989.209 kb on - strand, within AMB_RS04735 at 989.209 kb on - strand, within AMB_RS04735 at 989.209 kb on - strand, within AMB_RS04735 at 989.235 kb on + strand, within AMB_RS04735 at 989.235 kb on + strand, within AMB_RS04735 at 989.235 kb on + strand, within AMB_RS04735 at 989.236 kb on - strand, within AMB_RS04735 at 989.236 kb on - strand, within AMB_RS04735 at 989.236 kb on - strand, within AMB_RS04735 at 989.254 kb on + strand, within AMB_RS04735 at 989.254 kb on + strand, within AMB_RS04735 at 989.254 kb on + strand, within AMB_RS04735 at 989.406 kb on - strand, within AMB_RS04735 at 989.530 kb on - strand at 989.626 kb on - strand at 989.691 kb on + strand at 989.692 kb on - strand at 989.717 kb on + strand at 989.731 kb on + strand at 989.956 kb on + strand, within AMB_RS04740 at 989.956 kb on + strand, within AMB_RS04740 at 989.957 kb on - strand, within AMB_RS04740 at 989.971 kb on + strand, within AMB_RS04740 at 989.971 kb on + strand, within AMB_RS04740 at 989.972 kb on - strand, within AMB_RS04740 at 989.972 kb on - strand, within AMB_RS04740 at 989.972 kb on - strand, within AMB_RS04740 at 989.972 kb on - strand, within AMB_RS04740 at 990.132 kb on + strand at 990.132 kb on + strand at 990.133 kb on - strand at 990.133 kb on - strand at 990.163 kb on + strand at 990.163 kb on + strand at 990.163 kb on + strand at 990.163 kb on + strand at 990.163 kb on + strand at 990.164 kb on - strand at 990.164 kb on - strand at 990.164 kb on - strand at 990.423 kb on + strand, within AMB_RS23160 at 990.423 kb on + strand, within AMB_RS23160 at 990.423 kb on + strand, within AMB_RS23160 at 990.861 kb on + strand, within AMB_RS23160 at 990.862 kb on - strand, within AMB_RS23160 at 990.862 kb on - strand, within AMB_RS23160 at 990.862 kb on - strand, within AMB_RS23160 at 990.862 kb on - strand, within AMB_RS23160 at 990.921 kb on + strand, within AMB_RS23160 at 990.921 kb on + strand, within AMB_RS23160
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 988,771 - AMB_RS04735 0.44 -0.2 988,771 - AMB_RS04735 0.44 -0.0 988,814 + AMB_RS04735 0.47 +0.7 988,814 + AMB_RS04735 0.47 -0.0 988,815 - AMB_RS04735 0.47 +1.4 988,815 - AMB_RS04735 0.47 -0.0 988,878 - AMB_RS04735 0.51 +0.6 988,946 + AMB_RS04735 0.55 -1.5 988,947 - AMB_RS04735 0.55 +0.2 988,947 - AMB_RS04735 0.55 +0.0 988,997 + AMB_RS04735 0.59 +0.9 988,998 - AMB_RS04735 0.59 -0.0 988,998 - AMB_RS04735 0.59 -0.2 989,003 + AMB_RS04735 0.59 -0.2 989,003 + AMB_RS04735 0.59 +0.2 989,004 - AMB_RS04735 0.59 +0.6 989,118 + AMB_RS04735 0.67 -1.9 989,118 + AMB_RS04735 0.67 -0.7 989,118 + AMB_RS04735 0.67 +0.0 989,119 - AMB_RS04735 0.67 -0.3 989,150 + AMB_RS04735 0.69 -0.8 989,150 + AMB_RS04735 0.69 +0.5 989,150 + AMB_RS04735 0.69 -0.0 989,150 + AMB_RS04735 0.69 +0.1 989,150 + AMB_RS04735 0.69 +0.1 989,151 - AMB_RS04735 0.69 -0.0 989,151 - AMB_RS04735 0.69 -0.3 989,151 - AMB_RS04735 0.69 -0.1 989,153 + AMB_RS04735 0.69 +0.2 989,153 + AMB_RS04735 0.69 -0.2 989,208 + AMB_RS04735 0.73 -1.6 989,209 - AMB_RS04735 0.73 +0.4 989,209 - AMB_RS04735 0.73 -0.8 989,209 - AMB_RS04735 0.73 +0.2 989,235 + AMB_RS04735 0.74 -0.6 989,235 + AMB_RS04735 0.74 -0.5 989,235 + AMB_RS04735 0.74 -0.4 989,236 - AMB_RS04735 0.74 -0.5 989,236 - AMB_RS04735 0.74 -0.0 989,236 - AMB_RS04735 0.74 +0.1 989,254 + AMB_RS04735 0.76 -0.6 989,254 + AMB_RS04735 0.76 -0.7 989,254 + AMB_RS04735 0.76 +0.3 989,406 - AMB_RS04735 0.85 +0.3 989,530 - +0.3 989,626 - +0.3 989,691 + -0.6 989,692 - -2.4 989,717 + -0.5 989,731 + -1.6 989,956 + AMB_RS04740 0.54 -0.3 989,956 + AMB_RS04740 0.54 -2.6 989,957 - AMB_RS04740 0.54 -0.5 989,971 + AMB_RS04740 0.57 -1.4 989,971 + AMB_RS04740 0.57 -0.2 989,972 - AMB_RS04740 0.58 +0.2 989,972 - AMB_RS04740 0.58 -1.0 989,972 - AMB_RS04740 0.58 -2.7 989,972 - AMB_RS04740 0.58 -0.3 990,132 + +0.2 990,132 + -1.1 990,133 - -0.2 990,133 - -0.3 990,163 + -0.3 990,163 + -0.1 990,163 + -0.5 990,163 + +0.5 990,163 + +0.5 990,164 - -0.3 990,164 - -0.4 990,164 - -0.8 990,423 + AMB_RS23160 0.11 -0.0 990,423 + AMB_RS23160 0.11 +0.4 990,423 + AMB_RS23160 0.11 -1.7 990,861 + AMB_RS23160 0.35 -0.1 990,862 - AMB_RS23160 0.35 +0.1 990,862 - AMB_RS23160 0.35 -0.4 990,862 - AMB_RS23160 0.35 -1.9 990,862 - AMB_RS23160 0.35 -0.1 990,921 + AMB_RS23160 0.39 +1.0 990,921 + AMB_RS23160 0.39 -0.9
Or see this region's nucleotide sequence