Experiment: 30_initial_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04670 and AMB_RS04675 are separated by 27 nucleotides AMB_RS04675 and AMB_RS04680 are separated by 128 nucleotides AMB_RS04680 and AMB_RS04685 are separated by 169 nucleotides AMB_RS04685 and AMB_RS04690 are separated by 150 nucleotides
AMB_RS04670: AMB_RS04670 - signal transduction histidine kinase, at 977,962 to 978,675
_RS04670
AMB_RS04675: AMB_RS04675 - hypothetical protein, at 978,703 to 978,891
_RS04675
AMB_RS04680: AMB_RS04680 - transposase, at 979,020 to 979,286
_RS04680
AMB_RS04685: AMB_RS04685 - DNA-binding response regulator, at 979,456 to 980,070
_RS04685
AMB_RS04690: AMB_RS04690 - hypothetical protein, at 980,221 to 980,460
_RS04690
Position (kb)
979
980 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 978.098 kb on + strand, within AMB_RS04670 at 978.098 kb on + strand, within AMB_RS04670 at 978.149 kb on + strand, within AMB_RS04670 at 978.150 kb on - strand, within AMB_RS04670 at 978.238 kb on + strand, within AMB_RS04670 at 978.238 kb on + strand, within AMB_RS04670 at 978.238 kb on + strand, within AMB_RS04670 at 978.238 kb on + strand, within AMB_RS04670 at 978.238 kb on + strand, within AMB_RS04670 at 978.239 kb on - strand, within AMB_RS04670 at 978.239 kb on - strand, within AMB_RS04670 at 978.239 kb on - strand, within AMB_RS04670 at 978.239 kb on - strand, within AMB_RS04670 at 978.387 kb on + strand, within AMB_RS04670 at 978.387 kb on + strand, within AMB_RS04670 at 978.387 kb on + strand, within AMB_RS04670 at 978.387 kb on + strand, within AMB_RS04670 at 978.387 kb on + strand, within AMB_RS04670 at 978.387 kb on + strand, within AMB_RS04670 at 978.388 kb on - strand, within AMB_RS04670 at 978.388 kb on - strand, within AMB_RS04670 at 978.388 kb on - strand, within AMB_RS04670 at 978.388 kb on - strand, within AMB_RS04670 at 978.391 kb on + strand, within AMB_RS04670 at 978.392 kb on - strand, within AMB_RS04670 at 978.432 kb on + strand, within AMB_RS04670 at 978.432 kb on + strand, within AMB_RS04670 at 978.432 kb on + strand, within AMB_RS04670 at 978.433 kb on - strand, within AMB_RS04670 at 978.433 kb on - strand, within AMB_RS04670 at 978.447 kb on + strand, within AMB_RS04670 at 978.447 kb on + strand, within AMB_RS04670 at 978.460 kb on + strand, within AMB_RS04670 at 978.522 kb on + strand, within AMB_RS04670 at 978.522 kb on + strand, within AMB_RS04670 at 978.522 kb on + strand, within AMB_RS04670 at 978.581 kb on + strand, within AMB_RS04670 at 978.581 kb on + strand, within AMB_RS04670 at 978.582 kb on - strand, within AMB_RS04670 at 978.582 kb on - strand, within AMB_RS04670 at 978.582 kb on - strand, within AMB_RS04670 at 978.582 kb on - strand, within AMB_RS04670 at 978.629 kb on + strand at 978.629 kb on + strand at 978.629 kb on + strand at 978.629 kb on + strand at 978.629 kb on + strand at 978.629 kb on + strand at 978.629 kb on + strand at 978.629 kb on + strand at 978.629 kb on + strand at 978.630 kb on - strand at 978.630 kb on - strand at 978.630 kb on - strand at 978.630 kb on - strand at 978.630 kb on - strand at 978.655 kb on + strand at 978.656 kb on - strand at 978.656 kb on - strand at 978.976 kb on + strand at 978.977 kb on - strand at 979.003 kb on - strand at 979.153 kb on + strand, within AMB_RS04680 at 979.153 kb on + strand, within AMB_RS04680 at 979.154 kb on - strand, within AMB_RS04680 at 979.171 kb on + strand, within AMB_RS04680 at 979.171 kb on + strand, within AMB_RS04680 at 979.286 kb on + strand at 979.286 kb on + strand at 979.286 kb on + strand at 979.286 kb on + strand at 979.286 kb on + strand at 979.286 kb on + strand at 979.286 kb on + strand at 979.286 kb on + strand at 979.287 kb on - strand at 979.287 kb on - strand at 979.287 kb on - strand at 979.287 kb on - strand at 979.287 kb on - strand at 979.315 kb on + strand at 979.316 kb on - strand at 979.330 kb on - strand at 979.376 kb on - strand at 979.445 kb on + strand at 979.445 kb on + strand at 979.457 kb on + strand at 979.483 kb on - strand at 979.504 kb on + strand at 979.505 kb on - strand at 979.507 kb on + strand at 979.508 kb on - strand at 979.558 kb on - strand, within AMB_RS04685 at 979.561 kb on + strand, within AMB_RS04685 at 979.561 kb on + strand, within AMB_RS04685 at 979.562 kb on - strand, within AMB_RS04685 at 979.588 kb on + strand, within AMB_RS04685 at 979.629 kb on + strand, within AMB_RS04685 at 979.629 kb on + strand, within AMB_RS04685 at 979.630 kb on - strand, within AMB_RS04685 at 979.697 kb on + strand, within AMB_RS04685 at 979.697 kb on + strand, within AMB_RS04685 at 979.724 kb on - strand, within AMB_RS04685 at 979.759 kb on - strand, within AMB_RS04685 at 979.788 kb on + strand, within AMB_RS04685 at 979.831 kb on + strand, within AMB_RS04685 at 980.003 kb on + strand, within AMB_RS04685 at 980.003 kb on + strand, within AMB_RS04685 at 980.058 kb on + strand at 980.058 kb on + strand at 980.059 kb on - strand at 980.108 kb on - strand at 980.191 kb on + strand at 980.191 kb on + strand at 980.191 kb on + strand at 980.193 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_initial_2 remove 978,098 + AMB_RS04670 0.19 -0.4 978,098 + AMB_RS04670 0.19 -0.3 978,149 + AMB_RS04670 0.26 -0.1 978,150 - AMB_RS04670 0.26 +0.3 978,238 + AMB_RS04670 0.39 +0.5 978,238 + AMB_RS04670 0.39 -0.0 978,238 + AMB_RS04670 0.39 -0.8 978,238 + AMB_RS04670 0.39 -0.5 978,238 + AMB_RS04670 0.39 +0.1 978,239 - AMB_RS04670 0.39 -0.0 978,239 - AMB_RS04670 0.39 -1.5 978,239 - AMB_RS04670 0.39 -0.3 978,239 - AMB_RS04670 0.39 -0.0 978,387 + AMB_RS04670 0.60 -0.3 978,387 + AMB_RS04670 0.60 -0.0 978,387 + AMB_RS04670 0.60 +0.6 978,387 + AMB_RS04670 0.60 -0.6 978,387 + AMB_RS04670 0.60 -0.5 978,387 + AMB_RS04670 0.60 -0.9 978,388 - AMB_RS04670 0.60 -0.1 978,388 - AMB_RS04670 0.60 -0.1 978,388 - AMB_RS04670 0.60 -0.3 978,388 - AMB_RS04670 0.60 -0.8 978,391 + AMB_RS04670 0.60 -0.2 978,392 - AMB_RS04670 0.60 -1.8 978,432 + AMB_RS04670 0.66 -0.3 978,432 + AMB_RS04670 0.66 -1.2 978,432 + AMB_RS04670 0.66 -0.6 978,433 - AMB_RS04670 0.66 -0.1 978,433 - AMB_RS04670 0.66 +1.0 978,447 + AMB_RS04670 0.68 -0.4 978,447 + AMB_RS04670 0.68 +0.2 978,460 + AMB_RS04670 0.70 +0.4 978,522 + AMB_RS04670 0.78 +0.5 978,522 + AMB_RS04670 0.78 -0.5 978,522 + AMB_RS04670 0.78 +0.3 978,581 + AMB_RS04670 0.87 -1.5 978,581 + AMB_RS04670 0.87 -0.1 978,582 - AMB_RS04670 0.87 -0.4 978,582 - AMB_RS04670 0.87 -2.4 978,582 - AMB_RS04670 0.87 +0.0 978,582 - AMB_RS04670 0.87 -1.0 978,629 + -0.9 978,629 + +0.3 978,629 + -2.7 978,629 + -0.3 978,629 + +0.3 978,629 + -0.4 978,629 + -0.2 978,629 + -1.5 978,629 + -0.3 978,630 - +0.2 978,630 - -0.9 978,630 - +0.2 978,630 - -0.1 978,630 - -0.5 978,655 + +0.2 978,656 - -0.1 978,656 - -0.2 978,976 + -0.6 978,977 - -1.0 979,003 - +1.0 979,153 + AMB_RS04680 0.50 -0.8 979,153 + AMB_RS04680 0.50 -0.5 979,154 - AMB_RS04680 0.50 -0.7 979,171 + AMB_RS04680 0.57 -1.9 979,171 + AMB_RS04680 0.57 -0.0 979,286 + -0.0 979,286 + -0.2 979,286 + +0.5 979,286 + -1.2 979,286 + -0.8 979,286 + -0.3 979,286 + -0.8 979,286 + -0.2 979,287 - -0.0 979,287 - -0.8 979,287 - -2.5 979,287 - -0.3 979,287 - -0.2 979,315 + -1.0 979,316 - -0.4 979,330 - -0.1 979,376 - -0.2 979,445 + -0.5 979,445 + +0.9 979,457 + -0.7 979,483 - +0.1 979,504 + -0.3 979,505 - -0.0 979,507 + -1.4 979,508 - +0.1 979,558 - AMB_RS04685 0.17 -4.1 979,561 + AMB_RS04685 0.17 -0.0 979,561 + AMB_RS04685 0.17 -0.6 979,562 - AMB_RS04685 0.17 -0.9 979,588 + AMB_RS04685 0.21 -0.9 979,629 + AMB_RS04685 0.28 -0.5 979,629 + AMB_RS04685 0.28 -0.5 979,630 - AMB_RS04685 0.28 -2.0 979,697 + AMB_RS04685 0.39 +1.0 979,697 + AMB_RS04685 0.39 -0.4 979,724 - AMB_RS04685 0.44 -0.6 979,759 - AMB_RS04685 0.49 -0.0 979,788 + AMB_RS04685 0.54 -0.4 979,831 + AMB_RS04685 0.61 +0.0 980,003 + AMB_RS04685 0.89 +2.1 980,003 + AMB_RS04685 0.89 -0.4 980,058 + -1.2 980,058 + -0.4 980,059 - +0.2 980,108 - -2.9 980,191 + -0.1 980,191 + +0.4 980,191 + -1.0 980,193 + -0.6
Or see this region's nucleotide sequence